PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L.) TERHADAP JUMLAH SEL SPERMATOGENIK TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIINDUKSI MONOSODIUM GLUTAMAT

Mahidin, Mahidin; Maulana, Andi Muh.; Susiyadi, Susiyadi

doi:10.30595/hmj.v1i1.2480

Cited by 3 publications

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“…Pada penelitian Sumarni et al 2 menyatakan bahwa ekstrak kemangi dapat melarutkan batu ginjal kalsium, dimana uji kelarutan menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kemangi maka semakin besar kelarutannya. Pada penelitian yang dilakukan oleh Mahidin 3 di dapatkan bahwa pemberian ekstrak daun kemangi dapat memperbaiki sel spermatogenik yang rusak akibat pemberian MSG. Kemangi digunakan untuk terapi dalam mengobati pilek, demam, migrain, diabetes, kram menstruasi, sinusitis, penyakit kardiovaskular, nyeri saraf, gigitan serangga, dan sakit kepala, anti-hiperlipidemia 4,5,6 .…”

Section: Pendahuluanunclassified

PENGARUH EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR β2 MIKROGLOBULIN, ASAM URAT DAN GAMBARAN HISTOLOGI GINJAL PADA TIKUS PUTIH GALUR WISTAR (Rattus novergicus strain Wistar) YANG DIINDUKSI MONOSODIUM GLUTAMAT

Septianawati

Hernayanti²,

W³

2020

HMJ

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Basil leaves (Ocimum basilicum) is one of the traditional medicinal plants that is used as medicine.This study aims to determine the effect of giving basil leaves on levels of Beta 2 microglobulin, uric acid and kidney histology in white wistar-induced rats in MSG and determine the effective dose of basil leaves in an effort to improve kidney function.This study used 25 male Wistar strain rats with body weight 200-230 g. The test animal group was divided into five groups based on the stage of treatment. The first treatment group was a control group consisting of five mice that were not given basil leaf extract or MSG, the second treatment group was the group that was only given MSG without being given basil leaf extract, the third treatment group was the group given MSG followed by basil leaf extract dose 87, 5 mg / kgBB, the fourth treatment group was the group that was given MSG followed by the administration of basil leaf extract with a dose of 175 mg / kgBW, and the fifth treatment group was the group that was given MSG followed by the administration of basil leaf extract at a dose of 350 mg / kgBB. At the end of the treatment, rats were drawn for blood analysis of B2M levels and uric acid levels and kidney tissue uptake, as well as analyzing kidney tissue histology.The results showed that there were differences between Beta 2 microglobulin, uric acid and kidney histopathology of rats receiving basil leaf extract compared with MSG group without basil leaf extract. This study concludes that consuming basil leaf extract can reduce levels of B2M and uric acid and improve kidney histology induced by MSG.

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Section: Pendahuluanunclassified

PENGARUH EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR β2 MIKROGLOBULIN, ASAM URAT DAN GAMBARAN HISTOLOGI GINJAL PADA TIKUS PUTIH GALUR WISTAR (Rattus novergicus strain Wistar) YANG DIINDUKSI MONOSODIUM GLUTAMAT

Septianawati

Hernayanti²,

W³

2020

HMJ

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“…The excessive usage of MSG may have adverse effects all over the body due to the formation of free radicals and increased oxidative stress (Hamza & Al-Baqami, 2019). Likewise, the formation of free radicals causes many hazards to the testis tissue and several cells in the body such as cells of the hypothalamic-pituitary-gonadal axis as well as Leydig and Sertoli cells ( Mahidin et al, 2018;Martin-Hialgo et al, 2019). Recently, medicinal plants attracted attention for their antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

Possible Ameliorative Role of Ethanolic Ginger Extract (Zingiber officinale) against Testicular Toxicity Induced By Monosodium Glutamate In Albino Rats

Mohamed

Kamel

2021

Egyptian Academic Journal of Biological Sciences, B. Zoology

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The paper aims to investigate the protective effect of ethanolic ginger extract against Monosodium glutamate (MSG) induced reproductive disorders in male rats. Forty-eight male albino rats were separated into four experimental groups: normal control, ginger extract, MSG only, and MSG plus ginger extract groups. All experimental regimens were administrated orally for four weeks. Dissection was done after two and four weeks from the starting of the experiment. Body and testis weight, sperm abnormalities, biochemical investigations were recorded.Additionally, molecular, histopathological, and immunohistochemical techniques in testis tissue were performed. The present data shows a significant increase in body weight, sperm abnormalities, and serum total cholesterol, with a significant decline in testis weight, serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), and testosterone levels after MSG administration. There was an upregulation in the expression of Bax protein whereas downregulation in the expression of B-cell lymphoma-2 (BCL-2) protein in the MSG group when compared with the normal control. Furthermore, testicular alterations were distinguished in the group treated with MSG only, along with enhanced caspase-3 and declined BCl-2 positive cells in testis tissue. While administration with ethanolic ginger extract showed an ameliorative effect contrasted with testicular alterations produced by Monosodium glutamate. Study Protocol:Rats were left for one week as an acclimatization period before treatment. The animals were separated into 4 experimental groups (12 animal/group). The first group served as the normal control group which was given distilled water. The second group was given Ginger Extract (GE ) only at 50 mg/kg b.wt according to (Bordbar et al., 2013), while the third group received MSG only at 2 mg/g b.wt according to (Igwebuike et al., 2011). Finally, the 4 th group was received GE at 50 mg/kg b.wt, and MSG at 2 mg/g b.wt. All experimental regimens were given orally to rats for 4 consecutive weeks daily, animals were dissected after two and four weeks from the beginning of the experiment. Samples Collection:After two and four weeks, the weight of each animal was documented, then all rats were autopsied through diethyl ether (Sigma, USA) inhalation anesthesia, after which animals were carefully dissected. Epididymis was removed for sperm abnormalities and testes weights were recorded. Blood was collected by cardiac acupuncture in centrifuge tubes left to clot and centrifuged at 3000 rpm for twenty minutes. then serum was collected and kept at −20°C till usage. All animals tests were collected for molecular and histochemical studies. Sperm Head Abnormality Test:Sperm-shape abnormalities were made according to the method described by Wyrobek ( 1978). In detail, caudal epididymis was sited in a Petri-dish containing one ml of NaCl (0.9 %). The epididymis was cut off into small pieces to allow the sperms to get out. Then, three drops of this solution were used to prepare the smears. The sl...

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“…In addition, ROS may directly damage Leydig cells and Sertoli cells. (Mahidin, Maulana and Susiyadi, 2018). Therefore, it is necessary to administer an antioxidant to reduce or even prevent excessive ROS production resulting from exposure to MSG.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

Rahmawati

Nasihun

Isradji

2019

Sains Med. J. Kedokt. dan Kesehat.

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Introduction: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration. Objective: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats. Methods: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method. Results: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05. The Leydig cells and Sertoli cells counts and sperm quality of CM10-G are significantly higher than those of CM8-G, p<0.05. Conclusion: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.

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PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L.) TERHADAP JUMLAH SEL SPERMATOGENIK TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIINDUKSI MONOSODIUM GLUTAMAT

Cited by 3 publications

References 0 publications

PENGARUH EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR β2 MIKROGLOBULIN, ASAM URAT DAN GAMBARAN HISTOLOGI GINJAL PADA TIKUS PUTIH GALUR WISTAR (Rattus novergicus strain Wistar) YANG DIINDUKSI MONOSODIUM GLUTAMAT

PENGARUH EKSTRAK ETANOL DAUN KEMANGI (Ocimum bacilicum L.) TERHADAP KADAR β2 MIKROGLOBULIN, ASAM URAT DAN GAMBARAN HISTOLOGI GINJAL PADA TIKUS PUTIH GALUR WISTAR (Rattus novergicus strain Wistar) YANG DIINDUKSI MONOSODIUM GLUTAMAT

Possible Ameliorative Role of Ethanolic Ginger Extract (Zingiber officinale) against Testicular Toxicity Induced By Monosodium Glutamate In Albino Rats

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

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