Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In Vitro

Abstract

-Pacet, Cianjur 43253 Naskah diterima tanggal 10 Januari 2012 dan disetujui untuk diterbitkan tanggal 4 Juni 2012 ABSTRAK. Anggrek Dendrobium merupakan tanaman hias komersial yang sangat penting di Indonesia. Optimasi media dalam kultur in vitro sangat diperlukan untuk meningkatkan dan mempercepat pertumbuhan planlet. Salah satu cara untuk mengoptimalisasi media in vitro yaitu dengan pemberian myoinositol dan arang aktif. Tujuan penelitian ialah mengetahui pengaruh myoinositol dan arang aktif terhadap pertumbuhan planlet Dendrobium. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Hias Pasarminggu, Jakarta mulai Bulan Juni sampai dengan Desember 2010. Metode penelitian menggunakan rancangan acak kelompok dengan delapan perlakuan dan lima ulangan. Perlakuan terdiri atas konsentrasi myoinositol 0, 50, 100, dan 150 mg/l dengan dan tanpa penambahan arang aktif. Hasil penelitian menunjukkan bahwa pemberian myoinositol 50 mg/l tanpa arang aktif dapat meningkatkan tinggi planlet, panjang dan lebar daun, sedangkan myoinositol 100 mg/l dengan penambahan arang aktif 2 g/l meningkatkan pertumbuhan jumlah dan panjang akar terbaik. This study was aims to determine the effect of myoinositol and activated charcoal on the growth of Dendrobium plantlets. Dendrobium is one of the most important commercial orchids in Indonesia. Media optimization is critical factor to improve and to promote plantlet growth. One of the methods to enrich the medium was by the use of myoinositol and activated charcoal. The study was conducted at the In Vitro Culture Laboratory of Indonesian Ornamental Crops Plants Research Institute Pasarminggu, Jakarta from June through December 2010. A completely randomized design with eight treatments and five replications was used in this experiment. The treatments given were myoinositol 0, 50, 100, and 150 mg/l with and without addition of activated charcoal. The results showed that application of myoinositol 50 mg/l without activated charcoal enhance the plantlet height, length, and leave width, while myoinositol 100 mg/l with addition of activated charcoal 2 g/l increased the highest number of the root and the longest root growth.

New Dogashima Medium as Subculture Medium Improve the Growth of Vanda tricolor Shoots from Embryogenesis

“…One of the organic ingredients contained is myo-inositol. According to Widiastoety et al [26], myo-inositol plays a role in producing cell walls containing polysaccharides, lignin and protein. Myo-inositol also plays an important role in the structure of membranes in the nucleus, chloroplast, mitochondria, cytoplasm and endoplasm because myo-inositol is a water-soluble part of phospholipids.…”

The combination of murashige and skoog (MS) media and activated charcoal on the growth of the Vanda helvola orchid plant in vitro

“…The following research by Purwanto et al (2008) shows that the media treatment of ½ MS causes the highest average percentage of sprouted explants and is different from other treatments. Widiastoeti et al (2012) stated that activating charcoal in growing media can indicate the best root length growth. It is because activated charcoal in growing media can reduce the light that enters the media.…”

“…Tissue culture technique through seeds/embryos is done because the size of the seeds is very small or orchid seeds have no endosperm (food reserves), so it is necessary for human help so that the embryo can grow properly. In addition, tissue culture techniques aim to improve seedlings' uniformity in large quantities and relatively short (Widiastoeti et al, 2012) The success of tissue culture is determined mainly by the media used. The growing media commonly used for orchid germination are Vaccin and Went (VW) media, Knudson C (KC) media, and Murashinge and Skoog (MS) media.…”

INDUKSI TUNAS MIKRO PISANG KULTIVAR AMBON NANGKA (Musa sp.) SECARA IN VITRO

“…Penghambatan pertumbuhan karena penambahan arang aktif umumnya terjadi karena arang aktif dapat menyerap dan mengikat nutrisi, zat pengatur tumbuh NAA, kinetin, BAP, dan IAA pada media. Menurut Widiastoeti et al (2013), arang aktif dapat menyerap senyawa fenol yang keluar dari jaringan tanaman, selain menyerap bahan organik lain dalam media.…”