The research was carried out to determine the best concentration of kinetin and activated charcoal to stimulate the shoot growth of Ambon Nangka banana cultivar. This research used a Completely Randomised Design (CRD) arranged factorials. The first factor was the concentration of kinetin with 4 levels i.e. 15 μM, 20 μM, 25 μM and 30 μM. The second factor was the concentration of activated charcoal with 4 levels i.e. 0 gL -1 ; 1 gL -1 ; 2 gL -1 ; and 3 gL -1 . Each treatment combination was repeated 3 times, which resulted in 48 experimental units. The parameters measured were the shoot emergence time, number of shoots, shoot length, number of roots and root length. The data were analysed using an analysis of variance at 95% and 99% level of confidences. The research results showed that the interaction between kinetin and activated charcoal had no effect on shoot length of Ambon Nangka banana cultivar. It was also found that the addition of 20 µM kinetin without any added activated charcoal resulted in the highest shoot number of Ambon Nangka banana cultivar.
Krisan merupakan sejenis tumbuhan berbunga yang sering ditanam sebagai tanaman hiaspekarangan atau bunga petik. Seiring dengan bertambahnya jumlah penduduk, meningkatnyakesejahteraan masyarakat dan bertambahnya kesadaran masyarakat akan keindahan lingkunganmaka kebutuhan akan bunga krisan meningkat. Oleh karena itu untuk memenuhi kebutuhan akanbunga krisan dilakukan melalui kultur jaringan. Salah satu faktor yang dapat mempengaruhiterhadap pembiakan secara teknik kultur jaringan yaitu penambahan zat pengatur tumbuh. Dalampenelitian ini menggunakan zat pengatur tumbuh BAP (Benzile Amino Purin) dan IAA (Indole AseticAcid). Konsentrasi BAP terdiri 4 taraf yaitu 0 µM, 2,5 µM, 5 µM, dan 7,5 µM sedangkan konsentrasiIAA terdiri 4 taraf juga yaitu 0 µM, 1 µM, 2 µM, dan 3 µM. Tujuan dalam penelitian ini yaitu untukmengetahui pengaruh zat pengatur tumbuh BAP (Benzile Amino Purin) dan IAA (Indole Asetic Acid)terhadap jumlah daun, jumlah akar, jumlah tunas dan tinggi tanman. Untuk mengetahui tujuantersebut dilakukan pengujian dengan desain eksperimen faktorial. Hasil uji anava menunjukkanpada jumlah daun hanya konsentrasi zat pengatur tumbuh BAP yang signifikan artinya terdapatperbedaan pengaruh konsentrasi zat pengatur tumbuh BAP terhadap jumlah daun dan konsentrasiBAP yang optimal yaitu 7,5 µM. Dari hasil anava pada data jumlah akar menunjukkan zat pengaturtumbuh BAP, IAA, dan interaksi antara zat pengatur tumbuh BAP dan IAA signifikan. Pada ujirentang ganda Duncan menggunakan data jumlah akar menyimpulkan bahwa konsentrasi zatpengatur tumbuh yang baik atau optimal yaitu konsentrasi BAP 0 µM dan IAA 3 µM. Pada uji anavajumlah tunas dang tinggi bunga krisan sama-sama menunjukkan hanya zat pengatur tumbuh BAPyang signifikan dan uji rentang ganda Duncan zat pengatur tumbuh yang optimal terhadap jumlahtunas yaitu konsentrasi BAP 10 µM dan terhadap tinggi bunga krisan yaitu konsentrasi BAP 0 µM.
<p>Banana is a horticultural plant with very high potentials, which contains carbohydrates and vitamins that are useful in fulfilling people's food and nutritional needs. Hence, this study aims to produce superior banana seedlings and develop a protocol for their mass production using a plant <em>in vitro</em> culture technique. This was a two stage-experiment i.e. microshoot production and plantlet formation. The result showed that Gebyar cultivar produced more shoots than the Kepok Kuning<em> </em>cultivar, with an average of 4.25 microshoots explant<sup>-1</sup>. However, Kepok Kuning produced more leaves than Gebyar,<em> </em>with an average of 4.64 leaves plantlet<sup>-1</sup>. Banana shoots cultured on the media containing Indole-3-acetic acid (IAA) at a concentration of 2.5 µM produced the highest leaves number. Meanwhile, those cultured on the media containing 1-Naphthalenesacetic acid (NAA) at a concentration of 7.5 µM produced the highest roots number. A Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) up to 30 µM and the one supplemented with 7.5 μM of NAA are suitable for Kepok Kuning and Gebyar<em> </em>cultivars micropropagation with regard to microshoot induction and plantlet formation, respectively.</p>
<p><em><span lang="EN-US">Aquilaria malaccensis</span></em><span lang="EN-US"> Lamk</span><span lang="EN-US">.</span><span lang="EN-US"> is one of the most </span><span lang="EN-US">widespread</span><span lang="EN-US"> agarwood-producing plants </span><span lang="EN-US">that</span><span lang="EN-US"> face extinction due to overexploitation. Agarwood propagation using <em>in vitro </em>culture techniques is capable of producing large quantities of plants in a shorter time and free from pests and diseases. </span><span lang="EN-US">Therefore, this </span><span lang="EN-US">study</span><span lang="EN-US"> was conducted to analyze</span><span lang="EN-US"> the effect of auxins type and concentration on agarwood plantlet formation using a split-plot design. The main plot was the type of auxin which included IAA, IBA and NAA</span><span lang="EN-US">, while the</span><span lang="EN-US"> subplot was the concentration used which consisted of 0; 5; 10; 15 and 20 µM. The variable observed was agarwood plantlet formation with parameters measured including the number of shoots</span><span lang="EN-US"> and</span><span lang="EN-US"> leaves, plant height, and number of roots. The results showed that the formation of agarwood plantlets was controlled by the type, concentration, and interaction between the type and concentration of auxin. Furthermore, explants cultured on Murashige Skoog (MS) medium supplemented with 10 µM IBA produced the highest number of shoots (3.39 shoots explant<sup>-1</sup>) and leaves (7.25 leaves explants<sup>-1</sup>), while the addition of 10 uM NAA resulted in the highest number of roots (2.52 roots explant<sup>-1</sup>). This is the first time a study is conducted to evaluate the effect of type and concentration of auxins on agarwood plantlet formation</span><span lang="EN-US">.</span><span lang="EN-US"> The production of high-quality shoots and plantlets increased agarwood germplasm availability to prevent extinction and support sustainable production.</span></p>
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