Abstrak: Antiseptik adalah suatu senyawa kimia yang biasanya digunakan untuk menghambat maupun membunuh pertumbuhan mikroorganisme. Tanaman sirih merupakan salah satu tanaman yang mempunyai kandungan zat aktif yang dapat memberi efek antibakterial. Analisis yang dilakukan dalam penelitian ini meliputi analisis fisik, kimia dan sifat mikrobiologis. Formulasi gel antiseptik terbaik terdapat pada ekstrak daun sirih 25% dan TEA 0,75% (K1P2). Hasil pengujian terhadap sifat pH gel antiseptik menunjukkan bahwa pH gel antiseptik yang dihasilkan sesuai dengan SNI No. 4075-2:2017. Nilai viskositas gel antiseptik tertinggi diperoleh dari interaksi konsentrasi ekstrak daun sirih 35% dan konsentrasi TEA 1% yaitu 2551,07 cP. Seluruh formulasi gel antiseptik menunjukkan susunan yang homogen dan tidak terdapat butiran-butiran kasar. Hasil penilaian uji deskripsi pada perlakuan terbaik K1P2 menunjukkan warna kuning skala sangat kuat, aroma daun sirih skala sangat lemah, tekstur gel skala netral dan tekstur tidak lengket skala netral. Gel antiseptik ekstrak daun sirih memiliki aktivitas antibakteri terhadap bakteri S. aureus dan bakteri E. coli.Formulation Of Hand Antiseptic Gel from Betel Leaf (Piper Betle L.) Extract with the Addition of Triethanolamine (TEA) StabilizerAbstract: Antiseptics are chemical compounds that are usually used to inhibit or kill the growth of microorganisms. Betel plant is one of the plants that contain active substances that can have an antibacterial effect. The analysis carried out in this study includes the analysis of physical, chemical and microbiological properties. The best antiseptic gel formulation was found in 25% betel leaf extract and 0.75% TEA (K1P2). The test results on the pH properties of the antiseptic gel showed that the pH of the antiseptic gel produced was in accordance with SNI No. 4075-2:2017. The highest antiseptic gel viscosity value was obtained from the interaction of 35% betel leaf extract concentration and 1% TEA concentration, which was 2551.07 cP. All antiseptic gel formulations showed a homogeneous composition and there were no coarse grains. The results of the description test assessment on the best treatment K1P2 showed a very strong yellow color scale, a very weak scale of betel leaf aroma, a neutral scale gel texture and a neutral scale non-sticky texture. Betel leaf extract antiseptic gel has antibacterial activity against S. aureus and E. coli bacteria.