PENGARUH JENIS EKSPLAN DAN KOMBINASI ZAT PENGATUR TUMBUH (ZPT) TERHADAP INDUKSI KALUS Begonia bimaensis Undaharta &amp; Ardaka SECARA IN VITRO

Hendriyani, Ema; Warseno, Tri; Undaharta, Ni Kadek Erosi

doi:10.14203/bkr.v23i1.8

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Callus induction in Begonia bimaensis is carried out using leaf stalk, base, and blade explants. This rate of induction was different for each part, and no callus formation occurred in the leaf blade explants [11].…”

Section: Introductionmentioning

confidence: 94%

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

Suhartanto

Astutik

Umami

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Climate changes due to global warming affect the productivity of forage crops such as maize. However, the productivity of maize could be maximized by improving the plant’s adaptation and tolerance through breeding, using tissue culture. This study aims to determine the effect of explant types and light conditions on callus induction of srikandi putih maize. It was conducted at the Laboratory of Forage and Pasture Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta. Furthermore, it was carried out using a completely randomized design in a factorial pattern. There were two treatment factors namely explant types, which consisted of roots, stems, and leaves, and light conditions consisting of darkness and light. The results showed that callus induction occurred in the stem and root of explants at 60%, but not in the leaves. It also showed that the stem explants produced a higher fresh weight of callus at 239.6 mg compared to the roots which produced 77.6 mg. The dark condition also produced a 49.3% higher amount of callus with fresh weight of 115.1 mg. Meanwhile, the light condition produced 30.7% higher amounts of callus with fresh weight of 96.3 mg. It was concluded that all treatments showed a compact texture of callus, but the use of stem explants in dark conditions was the best treatment.

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Section: Introductionmentioning

confidence: 94%

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

Suhartanto

Astutik

Umami

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…The compact callus texture of the Dayak onion has a tight cell arrangement and forms a dense bulge. A compact callus structure was also reported in begonia callus that grown in 2,4-D + kinetin medium (Hendriyani et al, 2020). In this study, a callus growth analysis was also carried out to obtain the growth curve of Dayak onion callus on the best growth medium.…”

Section: Resultsmentioning

confidence: 73%

Callogenesis of Dayak Onion (Eleutherine palmifolia) Bulb in response of Picloram, 2,4-D, and Kinetin

Habibah,

Lutfiah,

Liana

et al. 2023

J Bio Bio Edu

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Dayak onion contains bioactive compounds that are traditionally used for medicine. The production of bioactive compounds can be increased through callus culture. This study aims to analyze the effect of the combination of plant growth regulator on the growth of Dayak bulb callus. The design of this study used a completely randomized factorial design with 2 factors. The first factor is the concentration of auxin. While the second factor is the concentration of kinetin. The parameters observed include the percentage of callus, the time of callus formation, fresh weight, dry weight, and callus morphology. In this study, a callus growth curve was also made to determine the best harvest time. The results showed that highest callus fresh weight was obtained in the P3K1 treatment. The percentage of callus formation in the picloram + kinetin treatment was higher than in the 2,4-D + kinetin treatment. The time of callus formation occurred in the combination of picloram + kinetin faster than the 2.4-D + kinetin treatment. The callus color is diverse, while the texture of the entire callus is compact. The best callus induction medium for Dayak onion bulbs is to use picloram 2-4 ppm + kinetin0.025-0.5 ppm. The results of this study provide optimal growth regulatory information for the induction of dayak onion callus that has never been reported before. This information can be the basis for the development of methods of production of bioactive compounds from dayak onions through callus culture.

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“…Kalus embriogenik dapat berkembang secara bertahap merupakan komponen yang penting pada tahap embriogenesis somatik tidak langsung [6]. Oleh karena itu, upaya dalam inisiasi kalus embriogenik yang efisien merupakan tahap penting dalam rangka mendapatkan bibit manggis Macakal dalam jumlah banyak [7].…”

Section: Pendahuluanunclassified

Inisiasi Kalus Embriogenik Manggis (Garcinia mangostana L.) var. Macakal Terhadap Pemberian 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyl Amino Purine Secara In Vitro

Negoro,

Susiyanti,

Isminingsih

et al. 2024

spibio

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Manggis Macakal merupakan varietas unggul yang berasal dari Banten. Produksi manggis nasional pada 2020-2022 mengalami kondisi yang fluktuatif, dikarenakan masih banyak masyarakat yang belum banyak membudidayakannya. Selain itu penting juga dilakukannya upaya untuk melestarikan plasma nutfah tanaman lokal Banten. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian 2,4-D dan BAP terhadap inisiasi kalus embriogenik manggis. Penelitian ini dilaksanakan di

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PENGARUH JENIS EKSPLAN DAN KOMBINASI ZAT PENGATUR TUMBUH (ZPT) TERHADAP INDUKSI KALUS Begonia bimaensis Undaharta & Ardaka SECARA IN VITRO

Cited by 4 publications

References 11 publications

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

Callogenesis of Dayak Onion (Eleutherine palmifolia) Bulb in response of Picloram, 2,4-D, and Kinetin

Inisiasi Kalus Embriogenik Manggis (Garcinia mangostana L.) var. Macakal Terhadap Pemberian 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyl Amino Purine Secara In Vitro

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