Penetration of Living Cell Membranes with Fortified Carbon Nanotube Tips

Vakarelski, Ivan U.; Brown, Scott C.; Higashitani, Ko; Moudgil, Brij M.

doi:10.1021/la701878n

Cited by 108 publications

(114 citation statements)

References 21 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…100 to 500 pN in the range of the size of 0.8 to 2.5 nm. Song et al (2011) also reported that the minimum force calculated from their unbiased MD simulations showed an agreement with an experimental result obtained from an atomic force microscopy (Vakarelski et al, 2007), although further investigations seem to be needed. They also calculated the minimum driving pressure, which was defined by the minimum force per cross-sectional area of the NP.…”

Section: Biased MD Simulation Studiessupporting

confidence: 57%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

View full text Add to dashboard Cite

Nanoparticles have attracted much attention as a key material for new biomedical and pharmaceutical applications. For success in these applications, the nanoparticles are required to translocate across the cell membrane and to reach to inside of the cell. Among several translocation pathways of nanoparticles, the direct permeation pathway has a great advantage due to its high delivery efficacy. However, despite many research efforts, key properties and factors for driving the direct permeation of nanoparticle and its underlying mechanisms are far from being understood. In this article, experimental and computational studies regarding the direct permeation of nanoparticles across a cell membrane will be reviewed. Firstly, experimental studies on the nanoparticle-cell interactions, where spontaneous direct permeation of nanoparticles was observed, are reviewed. From the experimental studies, potential key physico-chemical properties of nanoparticles for their direct permeation are discussed. Secondly, physical methods such as electroporation and sonoporation for delivering nanoparticles into cells are reviewed. Current status of technologies for facilitating the direct permeation of nanoparticle is presented. Finally, we review molecular dynamics simulation studies and present the latest findings on the underlying molecular mechanisms of the direct permeation of nanoparticle.

show abstract

Section: Biased MD Simulation Studiessupporting

confidence: 57%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

View full text Add to dashboard Cite

show abstract

“…Given that the diameter of dispersed MWCNTs was important in influencing mesothelial injury, we hypothesized that thin MWCNTs were cytotoxic by piercing the cell membrane, as the force to penetrate cell membranes has been found to be proportionally dependent on fiber diameter (30). For these studies, we defined internalization, phagocytosis, and piercing as follows: internalization as an active behavior of nonphagocytic cells (e.g., mesothelial cells) to engulf foreign materials, phagocytosis as an active process by phagocytes to engulf foreign materials, and piercing as an energy-independent penetration of cell membranes by fibrous materials that may or may not lead to translocation inside cells (depending on the type of material).…”

Section: Inverse Correlation Between Mwcnt Diameter and Mesothelial Cellmentioning

confidence: 99%

Diameter and rigidity of multiwalled carbon nanotubes are critical factors in mesothelial injury and carcinogenesis

Nagai

Okazaki

Chew

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

452

View full text Add to dashboard Cite

Multiwalled carbon nanotubes (MWCNTs) have the potential for widespread applications in engineering and materials science. However, because of their needle-like shape and high durability, concerns have been raised that MWCNTs may induce asbestos-like pathogenicity. Although recent studies have demonstrated that MWCNTs induce various types of reactivities, the physicochemical features of MWCNTs that determine their cytotoxicity and carcinogenicity in mesothelial cells remain unclear. Here, we showed that the deleterious effects of nonfunctionalized MWCNTs on human mesothelial cells were associated with their diameterdependent piercing of the cell membrane. Thin MWCNTs (diameter ∼ 50 nm) with high crystallinity showed mesothelial cell membrane piercing and cytotoxicity in vitro and subsequent inflammogenicity and mesotheliomagenicity in vivo. In contrast, thick (diameter ∼ 150 nm) or tangled (diameter ∼ 2-20 nm) MWCNTs were less toxic, inflammogenic, and carcinogenic. Thin and thick MWCNTs similarly affected macrophages. Mesotheliomas induced by MWCNTs shared homozygous deletion of Cdkn2a/2b tumor suppressor genes, similar to mesotheliomas induced by asbestos. Thus, we propose that different degrees of direct mesothelial injury by thin and thick MWCNTs are responsible for the extent of inflammogenicity and carcinogenicity. This work suggests that control of the diameter of MWCNTs could reduce the potential hazard to human health. environmental health | inflammation | nanotoxicology

show abstract

“…Several one-dimensional, nanotube-based probes developed recently [5][6][7][8] have been used for cell interrogation, but difficulties in probe fabrication and concerns about nanotube toxicity have hindered the use of carbon-based nanoprobes in cell physiology studies. To address these shortcomings, we have developed carbon nanopipettes (CNPs) capable of probing cells and injecting fluids without compromising cell viability [9].…”

Section: Introductionmentioning

confidence: 99%

Carbon nanopipettes characterize calcium release pathways in breast cancer cells

Schrlau¹,

Brǎiloiu²,

Patel³

et al. 2008

Nanotechnology

View full text Add to dashboard Cite

Carbon-based nanoprobes are attractive for minimally-invasive cell interrogation but their application in cell physiology has thus far been limited. We have developed carbon nanopipettes (CNPs) with nanoscopic tips and used them to inject calcium-mobilizing messengers into cells without compromising cell viability. We identify pathways sensitive to cyclic adenosine diphosphate ribose (cADPr) and nicotinic acid adenine dinucleotide phosphate (NAADP) in breast carcinoma cells. Our findings demonstrate the superior utility of CNPs for intracellular delivery of impermeant molecules and, more generally, for cell physiology studies. The CNPs do not appear to cause any lasting damage to cells. Their advantages over the commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as concurrent injection and electrical measurements.

show abstract

Penetration of Living Cell Membranes with Fortified Carbon Nanotube Tips

Cited by 108 publications

References 21 publications

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Diameter and rigidity of multiwalled carbon nanotubes are critical factors in mesothelial injury and carcinogenesis

Carbon nanopipettes characterize calcium release pathways in breast cancer cells

Contact Info

Product

Resources

About