Penetration of 14C-inulin and 14C-sucrose into brain, cerebrospinal fluid, and skeletal muscle of developing rats

Ferguson, Roger K.; Woodbury, Dixon M.

doi:10.1007/bf00239028

Cited by 173 publications

(86 citation statements)

References 21 publications

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“…Previous radioisotopic studies have shown that the barrier to most compounds is more developed in the mature animal. This is true for inulin (VERNADAKIS & WOODBURY, 1965;FERGUSON & WOODBURY. 1969).…”

mentioning

confidence: 82%

Blood-brain barrier: Selective changes during maturation

Purdy

Bondy

1976

Neuroscience

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mentioning

confidence: 82%

Blood-brain barrier: Selective changes during maturation

Purdy

Bondy

1976

Neuroscience

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“…In sheep, which are relatively mature at birth, the rate of penetration of inulin and su crose from plasma to brain has declined to the adult value by a gestational age of 130 days (Evans et aI., 1974). In the rat, the decline continues post natally and adult rates are reached at between 10 and 15 days of age (Ferguson and Woodbury, 1969). Junctional complexes between endothelial cells can be seen even in primitive capillaries of the devel oping brain (Donahue and Pappas, 1961;Caley and Maxwell, 1970).…”

Section: Adapative Changes During Developmentmentioning

confidence: 99%

Substrate Utilization and Brain Development

Cremer

1982

J Cereb Blood Flow Metab

167

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“…Ferguson & Woodbury, 1969;Dziegielewska et al 1979). In the present study, we have investigated in fetal sheep the exchange of lipid-insoluble molecules ranging in molecular size from sucrose (0-51 nm radius) to albumin (3 5 nm radius) between blood and CSF at three different sites: lateral ventricle, cisterna magna and dorsal cortical subarachnoid space.…”

Section: Introductionmentioning

confidence: 99%

Species‐specific transfer of plasma albumin from blood into different cerebrospinal fluid compartments in the fetal sheep.

Dzięgielewska

Habgood

Møllgård

et al. 1991

The Journal of Physiology

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SUMMARY1. The blood-cerebrospinal fluid (CSF) transfer of endogenous sheep albumin and several exogenous species of albumin has been investigated in different CSF compartments of the immature fetal sheep brain, at an early stage of development (60 days gestation, term is 150 days) when the CSF concentration of total protein is high.2. There were marked differences in the steady-state CSF/plasma ratios for all species of albumin (including endogenous sheep albumin) between different CSF compartments. Ratios measured in the cisterna magna were significantly higher than those in the dorsal subarachnoid space, which in turn were higher than those in the lateral ventricles. The ratios for endogenous sheep albumin were (%; mean + S.E.M.): lateral ventricle (LV), 4 0 + 0 03; dorsal subarachnoid (DSA), 61 + 10; cisterna magna (CM), 13-7+08. 3. Three hours after i.v. injection, the CSF/plasma ratios for bovine albumin (LV, 2 0 + 0-2; DSA, 2 4 + 0-1; CM, 7 2 + 0-7 %) were significantly lower than the ratio for endogenous sheep albumin in all three compartments. The ratios for human albumin (LV, 0 7 + 0 2; DSA, 1P0 + 0-2; CM, 3 9 + 0-4 %) were significantly lower than those for bovine albumin.4. In all three CSF compartments, the endogenous sheep albumin ratios were higher than would be expected on the basis of transfer by passive mechanisms.Conversely, steady-state CSF/plasma ratios for [3H]sucrose and [14C]inulin were consistent with passive transfer, and there were no differences between the ratios for these markers measured in each of the three CSF regions. 7. Immunocytochemical evidence suggests that the route of transfer from blood to CSF is transcellular, through the choroid plexus epithelial cells.8. Regional variations in albumin ratios are probably due to differences in specific transfer into each CSF compartment. This is reflected in a differential immunocytochemical staining for albumin in choroid plexus epithelial cells from different regions of the brain.9. The results are discussed in terms of differences in albumin amino acid sequences, structural homologies, and transfer by a specific transcellular mechanism.

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Penetration of 14C-inulin and 14C-sucrose into brain, cerebrospinal fluid, and skeletal muscle of developing rats

Cited by 173 publications

References 21 publications

Blood-brain barrier: Selective changes during maturation

Blood-brain barrier: Selective changes during maturation

Substrate Utilization and Brain Development

Species‐specific transfer of plasma albumin from blood into different cerebrospinal fluid compartments in the fetal sheep.

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