“…This value is similar to that of the paired control Jobe and also to the penetrance found in operated chick embryos of the same age [32]. Therefore, nonafferentation does not alter the development of the blood-brain barrier toward praline.…”
Section: Nutrient Supply Of the Optic Lobessupporting
confidence: 70%
“…The inulin space was estimated after intracardiac injection of a mixture of 14 C-antipyrine and 3H-inulin (875 mCi/ mM) as previously described [32]. The blood-brain barrier toward proline was estimated by a similar injection of a mixture of IJC-antipyrine and L-(5-3 H) proline (33 Cifmmol) (32], a method first described by Oldendor/ [30].…”
Section: Measure111e111 Of Cerebral Blood Flow /Nu/inmentioning
confidence: 99%
“…The blood-brain barrier toward proline was estimated by a similar injection of a mixture of IJC-antipyrine and L-(5-3 H) proline (33 Cifmmol) (32], a method first described by Oldendor/ [30]. Briefly, a mixture of 8 µCi of the 3f-I.compound and 0.17 µCi of MC-antipyrine in 0.14 M saline were injected into the exposed embryonic heart and optic lobes were dissected out JO sec later.…”
Section: Measure111e111 Of Cerebral Blood Flow /Nu/inmentioning
“…This value is similar to that of the paired control Jobe and also to the penetrance found in operated chick embryos of the same age [32]. Therefore, nonafferentation does not alter the development of the blood-brain barrier toward praline.…”
Section: Nutrient Supply Of the Optic Lobessupporting
confidence: 70%
“…The inulin space was estimated after intracardiac injection of a mixture of 14 C-antipyrine and 3H-inulin (875 mCi/ mM) as previously described [32]. The blood-brain barrier toward proline was estimated by a similar injection of a mixture of IJC-antipyrine and L-(5-3 H) proline (33 Cifmmol) (32], a method first described by Oldendor/ [30].…”
Section: Measure111e111 Of Cerebral Blood Flow /Nu/inmentioning
confidence: 99%
“…The blood-brain barrier toward proline was estimated by a similar injection of a mixture of IJC-antipyrine and L-(5-3 H) proline (33 Cifmmol) (32], a method first described by Oldendor/ [30]. Briefly, a mixture of 8 µCi of the 3f-I.compound and 0.17 µCi of MC-antipyrine in 0.14 M saline were injected into the exposed embryonic heart and optic lobes were dissected out JO sec later.…”
Section: Measure111e111 Of Cerebral Blood Flow /Nu/inmentioning
“…The rat BBB develops slowly, starting around embryonic day 11 (E11) and becoming mature between postnatal (P) weeks 3 and 4 (Johanson, 1980). The chick BBB, however, develops more rapidly, beginning at E9 (Delorme et al, 1970) and maturing around the time of hatching (Purdy and Bondy, 1976). If agrin is involved in barrier function, it likely accumulates with a time course similar to that described for the development of BBB properties in both rat and chick.…”
Section: Brain Microvascular Agrin Accumulates As the Blood-brain Barmentioning
“…During development of the BBB, selective mechanisms mature for the uptake or exclusion of various types of molecules in the central nervous system (10,11). Recently we have observed postnatal maturation of the BBB for unbound bilirubin in newborn piglets (12).…”
ABSTRACT. Permeability of the blood brain barrier (BBB) for bilirubin and '2SI-albumin was studied in 2-dand 2-wk-old piglets. '251-albumin was given by bolus at the beginning of each study. Hyperbilirubinemia was produced by an initial bolus infusion of bilirubin and sustained at a plasma bi1irubin:albumin molar ratio of approximately 1.0 by continuous infusion of bilirubin for 3 h. During the study period, arterial pH and blood gas tensions, serum osmolarity, and mean arterial blood pressure were within the physiologic range for age in both groups. Serum albumin and serum total and unbound bilirubin concentrations were higher in the 2-wk-old piglets. Brain bilirubin concentrations and permeability ( P . S product) of the BBB for bilirubin were higher in the 2-d-old than in the 2-wk-old piglets, but the values of P . S for albumin were not different between the two groups. In 2-d-old piglets, regional brain bilirubin concentrations and permeability of the BBB were higher in subcortical regions (cerebellum and brainstem) than in the cerebral cortex. Regional brain albumin concentrations and BBB permeability to albumin in 2-d-old piglets were higher only in the cerebellum. In all regions, the bi1irubin:albumin molar ratio was higher in the brain tissues than in the blood. In 2-wk-old piglets, the brain concentrations and P . S products for bilirubin were lower and the regional differences were less marked than for 2-d-old animals. We conclude that in 2-d-old piglets the blood brain barrier is more permeable to bilirubin than to albumin, that brainstem and cerebellum are more permeable to bilirubin than cortical regions, and that by 2 wk the permeability of the BBB to bilirubin decreases while permeability to albumin remains unchanged. (Pediatr Res 25:452-456, 1989) Abbreviations BBB, blood brain barrier P . S, permeability. surface area Hyperbilirubinemia is one of the most common problems in the newborn infant. Although the mechanism of bilirubin toxicity is not fully understood, kernicterus may result from the entry of the unbound fraction of unconjugated bilirubin into the brain (1, 2). Because in sick infants kernicterus can occur at very low levels of total serum bilirubin (1-3), it is also possible that immaturity of the blood-brain barrier or injury to it may allow entry of bilirubin into the brain (3-6). In most species of animals, hyperbilirubinemia alone fails to cause kernicterus (4-6), even at very high serum bilirubin concentrations (4-6). Kernicterus occurs more readily in asphyxiated animals (4) or in Gunn rats treated with sulfisoxazole (7). The free bilirubin theory as the sole explanation of kernicterus in low birth wt infants has also been challenged by recent observations that hyperosmolar opening of the BBB for albumin-bound bilirubin might play a role in the pathogenesis of kernicterus with serum bilirubin levels below the range of a saturated bilirubin capacity (6,8,9).Ehrlich introduced the concept of a blood brain banier when he reported in 1885 that aniline dyes injected into the blo...
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