Penetration and Uptake of Colloidal Gold‐Labeled Concanavalin A in The Junctional Epithelium of The Rat

Takata, Takashi; Nikai, Hiromasa; Ijuhin, Naokuni; Ito, Hiroshi

doi:10.1902/jop.1988.59.12.823

Cited by 19 publications

(7 citation statements)

References 10 publications

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“…6A), but only slightly. To explain this, it is reported that rat gingival junctional epithelium can uptake topically applied horseradish peroxidase or colloidal gold-labeled concanavalin A [24], [25]. Thus, the uptake of latex beads by BSA appears to have resulted from the intrinsic characteristics of epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement

et al. 2011

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The onset and progressive pathogenesis of periodontal disease is thought to be initiated by the entry of Aggregatibacter actinomycetemcomitans (Aa) into periodontal tissue, especially gingival epithelium. Nonetheless, the mechanism underlying such bacterial entry remains to be clarified. Therefore, this study aimed to investigate the possible role of Aa outer membrane protein 29 kD (Omp29), a homologue of E. coli OmpA, in promoting bacterial entry into gingival epithelial cells. To accomplish this, Omp29 expression vector was incorporated in an OmpA-deficient mutant of E. coli. Omp29+/OmpA− E. coli demonstrated 22-fold higher entry into human gingival epithelial line cells (OBA9) than Omp29−/OmpA− E. coli. While the entry of Aa and Omp29+/OmpA− E. coli into OBA9 cells were inhibited by anti-Omp29 antibody, their adherence to OBA9 cells was not inhibited. Stimulation of OBA9 cells with purified Omp29 increased the phosphorylation of focal adhesion kinase (FAK), a pivotal cell-signaling molecule that can up-regulate actin rearrangement. Furthermore, Omp29 increased the formation of F-actin in OBA9 cells. The internalization of Omp29-coated beads and the entry of Aa into OBA9 were partially inhibited by treatment with PI3-kinase inhibitor (Wortmannin) and Rho GTPases inhibitor (EDIN), both known to convey FAK-signaling to actin-rearrangement. These results suggest that Omp29 is associated with the entry of Aa into gingival epithelial cells by up-regulating F-actin rearrangement via the FAK signaling pathway.

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Section: Resultsmentioning

confidence: 99%

Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement

et al. 2011

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“…The junctional epithelium has high permeability because of the presence of wide intercellular spaces. These spaces allow various substances to pass in and out of the epithelial layer, as demonstrated by experimental studies using tracers (15–17). From the standpoint of a defense system, however, this high permeability is a disadvantageous factor in periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat molars

Tamura

Nakakura-Ohshima

Maeda

et al. 2003

J of Periodontal Research

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The distribution of immunocompetent cells in the dentogingival junction of rat molars during root formation was investigated by immunocytochemistry using antibodies to class II major histocompatibility complex (MHC) molecules (OX6-antibody) and monocyte/macrophage lineage cells (ED1-antibody) as well as by histochemical reaction for periodic acid-Schiff (PAS). Two portions (the junctional epithelium in the mesial gingiva of the first molar, and the interdental gingiva between the first and second molars) were selected for observations. At the eruption stage of the first molar (16-18 days after birth), OX6-positive cells, dendritic or oval in shape, were abundantly distributed in the connective tissue between the oral epithelium and tooth germ. Positive cells with slender cell processes were also found beneath the ameloblast layer. At the commencement stage of the first molar occlusion (24-28 days after birth), numerous OX6-positive cells displaying a dendritic fashion existed preferentially in the mesial gingiva, but were fewer in the interdental gingiva. In contrast, the interdental gingiva showed a denser distribution of ED1-positive cells and PAS-reactive polymorphonuclear leukocytes (PMLs) than the mesial gingiva. At the completion stage of root formation (100-120 days after birth), the OX6-immunopositive cells invaded the deeper position of the mesial gingiva with the downgrowth of the epithelium; they had a considerably higher cell density compared with those in the interdental gingiva where PAS-reactive PMLs persisted. These findings indicated that the immunocompetent cells showed a region-specific distribution and cell density by their roles in immune response.

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“…Gometz et al (20) also reported that inflammatory macrophages resided preferentially immediately adjacent to the sulcular epithelia. It is well known that the permeability of JE allows entry of various substances (8,21). Some irritants in the oral environment can infiltrate into the gingival connective tissue through the wide intercellular space of JE in the normal condition.…”

Section: Discussionmentioning

confidence: 99%

Distribution of macrophage lineage cells in rat gingival tissue after topical application of lipopolysaccharide: an immunohistochemical study using monoclonal antibodies: 0X6, ED1 and ED2

Miyauchi¹,

Takata²,

Ito³

et al. 1998

J of Periodontal Research

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To discuss the role of macrophage lineage cells on the periodontal tissue destruction, we immunohistochemically examined the phenotype and the dynamics of macrophage lineage cells 1 or 3 h or 1, 2, 3 or 7 d after topical application of LPS (5 mg/ml in physiological saline) from the rat gingival sulcus using 3 monoclonal antibodies: OX6 (antigen‐presenting cells), EDI (monocytes, macrophages and dendritic cells) and ED2 (resident macrophages). We could detect at least 3 different types of macrophage lineage cells, namely 0X6+ /ED1+ /ED2− dendritic cells and exudate macrophages and ED2+ resident macrophages. After LPS application the majority of macrophage lineage cells accumulated in the subjunctional epithelial area were newly extravasated 0X6+/EDI+/ED2− dendritic cells or macrophages. The number of these cells increased progressively with time and reached a maximum level at d 2. On the other hand, number and tissue distribution of ED2+ resident macrophages did not change. These results indicate that several types of macrophage lineage cells exist in rat gingival tissue and suggest that dendritic cells and exudate macrophages transiently accumulated after LPS application are responsible for various host immune response and tissue destruction caused by LPS.

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Penetration and Uptake of Colloidal Gold‐Labeled Concanavalin A in The Junctional Epithelium of The Rat

Cited by 19 publications

References 10 publications

Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement

Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement

Different distribution of immunocompetent cells in the dentogingival junction during root formation in rat molars

Distribution of macrophage lineage cells in rat gingival tissue after topical application of lipopolysaccharide: an immunohistochemical study using monoclonal antibodies: 0X6, ED1 and ED2

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