This study aims to produce an interactive e-module (e-MI) on carbohydrate material biochemistry course with good validity, practicality, and effectiveness. The research method used in this research is Research and Development (R&D) with ADDIE approach design (Analyze, Design, Development, Implementation, Evaluation). The results of this study are e-MI products based on the validation results of content experts by 84.25% and media experts by 77.98%, showing valid criteria in the aspects of content feasibility and practicality. While the results of user trials of 81.34% indicate practical criteria in the practical aspect of using interactive e-modules (e-MI), and a percentage of 82.65% shows very effective criteria in terms of the effectiveness of using e-MI. So the results of this study recommend using this interactive e-module (e-MI) for learning activities, especially in the Biochemistry course of carbohydrate material.