Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells

Chemical group-dependent plasma polymerisation preferentially directs adipose stem cell differentiation towards osteogenic or chondrogenic lineages

“…Functionalisation of carbon nanotubes with COOH was also shown to enhance bovine chondrocytes’ ability to express the chondrogenic marker, collagen II [23] . In contrast, Chen et al reported that CH 3, rather than NH 2 and COOH functional groups, promoted chondrogenesis of human amniotic membrane-derived MSCs [21] .…”

“…Liu et al also studied allylamine plasma coatings and suggested that this modification promotes attachment, spreading, proliferation and osteogenic differentiation of human ADSCs [20] . A study by Chen’s et al also indicated that NH 2 functional groups support osteogenesis of amniotic membrane-derived MSCs on PCL (poly(ε-caprolactone)) surfaces [21] . On the other hand, Wang et al found that acrylic acid with COOH functional groups supported osteogenic differentiation of rat BM-MSCs as indicated by calcium deposition and colony formation [22] .…”

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

“…The rationale behind the need for bottom‐up control of cell responses is derived from controlling cell function via directing interactions with a surface rather than taking this as benign or inevitable constant. Material induced control of biological responses encompasses research into the effect of surface chemistry, topography and stiffness on cellular responses. All of these approaches show potential, but fundamental systematic knowledge regarding the optimal combination of material properties required to initiate effective control over cell function is still lacking.…”

Poly(ε‐caprolactone)‐based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis

“…The series of PCL polymers bearing different pendant functional groups were prepared through the ring‐opening co‐polymerization between ε‐caprolactone and distinct co‐monomers (feeding molar ratio: 9/1) using benzyl alcohol as initiator and Sn(Oct) 2 as catalyst and characterized as described in our previous work . PCL polymers bearing ‐NH 2 , ‐CH 3 , ‐COOH, –OH and ‐C=O were designated as PCL‐NH 2 , PCL‐CH 3 , PCL‐COOH, PCL‐OH and PCL‐C=O in the following context, respectively.…”

“…Previously, we had developed a novel series of poly(ε‐caprolactone) polymers bearing different pendent chemical groups (i.e. hydroxyl, methyl, carboxyl, amino and carbonyl) and successfully applied in studying the behaviours of MSCs . The objective of this study was to perform a comparative investigation on different chondrogenic cells by employing such a biomaterials platform.…”