Poly(ε‐caprolactone)‐based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis

Chen, Min; Xu, Lei; Zhou, Yan; Zhang, Yan; Lang, Meidong; Ye, Zhaoyang; Tan, Wen‐Song

doi:10.1111/cpr.12272

Cited by 8 publications

(4 citation statements)

References 41 publications

(68 reference statements)

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“…While PCL scaffolds can support stem cell differentiation and proliferation, its hydrophobic profile inhibits cellular attachment and thus hurts its suitability in tissue engineering ( Sousa et al, 2014 ). Researchers have attempted to use chemical and plasma treatments together with blending of hydrophilic materials and the ECM to increase the PCL’s hydrophilicity ( Sousa et al, 2014 ; Chen et al, 2016 ; Silva et al, 2017 ). PCL-based fibrous scaffolds exhibit high structural integrity following incubation in a physiological solution, and support desirable cell responses of the seeded MSCs ( Liu et al, 2006 ).…”

Section: Synthetic Polymers As Scaffolds For Cartilage Tissue Engineementioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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Cartilage, especially articular cartilage, is a unique connective tissue consisting of chondrocytes and cartilage matrix that covers the surface of joints. It plays a critical role in maintaining joint durability and mobility by providing nearly frictionless articulation for mechanical load transmission between joints. Damage to the articular cartilage frequently results from sport-related injuries, systemic diseases, degeneration, trauma, or tumors. Failure to treat impaired cartilage may lead to osteoarthritis, affecting more than 25% of the adult population globally. Articular cartilage has a very low intrinsic self-repair capacity due to the limited proliferative ability of adult chondrocytes, lack of vascularization and innervation, slow matrix turnover, and low supply of progenitor cells. Furthermore, articular chondrocytes are encapsulated in low-nutrient, low-oxygen environment. While cartilage restoration techniques such as osteochondral transplantation, autologous chondrocyte implantation (ACI), and microfracture have been used to repair certain cartilage defects, the clinical outcomes are often mixed and undesirable. Cartilage tissue engineering (CTE) may hold promise to facilitate cartilage repair. Ideally, the prerequisites for successful CTE should include the use of effective chondrogenic factors, an ample supply of chondrogenic progenitors, and the employment of cell-friendly, biocompatible scaffold materials. Significant progress has been made on the above three fronts in past decade, which has been further facilitated by the advent of 3D bio-printing. In this review, we briefly discuss potential sources of chondrogenic progenitors. We then primarily focus on currently available chondrocyte-friendly scaffold materials, along with 3D bioprinting techniques, for their potential roles in effective CTE. It is hoped that this review will serve as a primer to bring cartilage biologists, synthetic chemists, biomechanical engineers, and 3D-bioprinting technologists together to expedite CTE process for eventual clinical applications.

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Section: Synthetic Polymers As Scaffolds For Cartilage Tissue Engineementioning

confidence: 99%

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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“…Besides pure PCL scaffolds, Fu et al fabricated PCL-PEG-PCL scaffold [7] to improve hydrophilicity. Chen et al developed positively charged PCL-NH2 [8] that promotes chondrogenesis. However, these modifications may change the mechanical properties and structure of scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration

Yao

et al. 2019

Med Sci Monit

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Background We synthetized a 3D printed poly-ɛ-caprolactone (PCL) scaffold with polydopamine (PDA) coating and lithium chloride (LiCl) deposition for cartilage tissue engineering and analyzed its effect on promoting rabbit bone marrow mesenchymal stem cells (rBMSC) chondrogenesis in vitro . Material/Methods PCL scaffolds were prepared by 3D printing with a well-designed CAD digital model, then modified by PDA coating to produce PCL-PDA scaffolds. Finally, LiCl was deposited on the PDA coating to produce PCL-PDA-Li scaffolds. The physicochemical properties, bioactivity, and biocompatibility of PCL-PDA-Li scaffolds were accessed by comparing them with PCL scaffolds and PCL-PDA scaffolds. Results 3D PCL scaffolds exhibited excellent mechanical integrity as designed. PDA coating and LiCl deposition improved surface hydrophilicity without sacrificing mechanical strength. Li + release was durable and ion concentration did not reach the cytotoxicity level. This in vitro study showed that, compared to PCL scaffolds, PCL-PDA and PCL-PDA-Li scaffolds significantly increased glycosaminoglycan (GAG) formation and chondrogenic marker gene expression, while PCL-PDA-Li scaffolds showed far higher rBMSC viability and chondrogenesis. Conclusions 3D printed PCL-PDA-Li scaffolds promoted chondrogenesis in vitro and may provide a good method for lithium administration and be a potential candidate for cartilage tissue engineering.

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“…However, these synthetic polymers generally lack bioactivity to stimulate cell and protein adhesion, which are necessary for an efficient cell differentiation and tissue formation. This is due to such polymers being relatively hydrophobic, with static contact angles in the range of 75°–90°, and lacking sufficient reactive side-chain groups for influencing cell behavior [56,57]. For this reason, research has been focused over the last two decades on augmenting the bioactivity of ME-AM scaffolds for BTE made of synthetic polymers.…”

Section: Bone Tissue Engineering (Bte) – Towards Additive Manufacture...mentioning

confidence: 99%

Boosting bone regeneration using augmented melt-extruded additive-manufactured scaffolds

Cámara-Torres

Fucile

Sinha

et al. 2023

International Materials Reviews

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Bone tissue engineering (BTE) is in active search of the ideal scaffold to give a clinical solution for bone regeneration in non-union fractures. During the last decades, the use of additive manufacturing (AM), and, in particular, melt extrusion AM (ME-AM), has been investigated towards this aim. ME-AM enables the fabrication of personalized 3D scaffolds, with a controlled and highly interconnected porosity, through the solvent-free processing of biodegradable and mechanically robust polymers. In addition to these properties matching the requirements for BTE scaffolds, the polymers used to fabricate these constructs are also more amenable for further functionalization than metals or ceramics, to influence cell behaviour, making thermoplastic materials a preferred choice for BTE. This review provides a comprehensive analysis of various ME-AM scaffolds developed for BTE, along with approaches used to augment their bioactivity, which includes architectural, surface physical and chemical modifications, the incorporation of secondary fibrous or hydrogel networks within the scaffold pores, and the use of composites for ME-AM scaffold fabrication.

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Poly(ε‐caprolactone)‐based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis

Abstract: This study demonstrates that different cells displayed distinct responses to substrate surface chemistry, implying that cell-biomaterial interactions can be developmental stage dependent. This provides a novel perspective for developing biomaterials for cartilage regeneration.

Cited by 8 publications

References 41 publications

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering

Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration

Boosting bone regeneration using augmented melt-extruded additive-manufactured scaffolds

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