Pen studies on the control of cattle tick (Rhipicephalus (Boophilus) microplus) with Metarhizium anisopliae (Sorokin)

Leemon, Diana M.; Turner, L; Jonsson, N.N.

doi:10.1016/j.vetpar.2008.06.007

Cited by 39 publications

(22 citation statements)

References 15 publications

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“…(B.) microplus Bittencourt, 2008;Lubeck et al, 2008;Gindin et al, 2009), being effective against ticks such as Ixodes scapularis, Oligonynchus yothersi, Rhipicephalus appendiculatus, R. sanguineus, Hyalomma excavatum, and Amblyomma variegatum under laboratory and field conditions (Oliveira et al, 2002;Leemon et al, 2008). The entomopathogenic B. bassiana is usually found infecting insects.…”

Section: Introductionmentioning

confidence: 99%

Endophytic and entomopathogenic strains of Beauveria sp to control the bovine tick Rhipicephalus (Boophilus) microplus

Campos¹,

Boldo²,

Pimentel³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Pathogenicity of strains of the entomopathogenic fungus Beauveria bassiana and endophytic strains of Beauveria sp against the bovine tick Rhipicephalus (Boophilus) microplus was tested in laboratory bioassays and under field conditions. Suspensions containing 10 5 , 10 7 and 10 9 conidia/mL were prepared of each fungal strain for laboratory bioassays. The ticks were maintained at 28°C, 90 ± 5% relative humidity, and the following variables were evaluated: initial female weight, egg weight, hatching percentage, reproductive efficiency, and percentage control. For tests under field conditions, a Beauveria suspension containing 10 6 conidia/mL was sprayed on tick- infested cows. After 72 h, the ticks were collected to estimate mortality under field conditions. Laboratory bioassays showed a mortality of 20 to 50% of the ticks seven days after inoculation with 10 7 Beauveria conidia/mL. Under field conditions 10 6 Beauveria conidia/mL induced 18-32% mortality. All Beauveria strains were effective in biological control of R. (Boophilus) microplus under laboratory and field test conditions. This is the first demonstration that endophytic fungi can be used for biological control of the cattle tick; this could help reduce environmental contamination by diminishing the need for chemical acaricides. Two endophytic strains were isolated from maize leaves and characterized by molecular sequencing of 5.8S rDNA ITS1 and ITS2 and morphological analyses of conidia. We found that these two endophytic Beauveria isolates, designated B95 and B157, are close to Beauveria amorpha.

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Section: Introductionmentioning

confidence: 99%

Endophytic and entomopathogenic strains of Beauveria sp to control the bovine tick Rhipicephalus (Boophilus) microplus

Campos¹,

Boldo²,

Pimentel³

et al. 2010

Genet. Mol. Res.

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“…Leemon et al (2008) reportan que las garrapatas tratadas con la cepa MaF1309® de Metarhizium anisopliae a una concentración 1 X 10 8 conidias/ml alcanzó 100% de mortalidad a los 14 días después del tratamiento, y que 50% de las garrapatas presentó fungosis a los 10, 18 y 22 días del tratamiento, lo cual concuerda con los resultados de este estudio con la cepa nativa MM01 a la misma concentración 1 X 10 8 conidias/ml, que presentó patogenicidad en las garrapatas a los 10 día de la aplicación, sin embargo, no coincide con el porcentaje de mortalidad posterior a la aplicación.…”

Section: Discussionunclassified

Control biológico de Rhicephalus (Boophilus) microplus con hongos entomopatógenos / Biological Control of Rhicephalus (Boophilus) microplus with Entomopathogenic Fungi

Gálvez

Segura

Gómez-Vázquez

2017

CIBA

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ResumenEn México el problema principal de la ganadería bovina, en lo particular en el trópico, es controlar las garrapatas Rhicephalus (Boophilus) microplus, ya que el uso inadecuado de los tratamientos químicos ha creado resistencia en esos parásitos. El control biológico en garrapatas es un concepto nuevo y ha sido una medida efectiva, que ha permitido mantener estas dentro de parámetros económicos y zoosanitarios aceptables. Una de las posibilidades reales en este campo para controlar garrapatas Rhicephalus (Boophilus) microplus de importancia pecuaria es el uso de hongos entomopatógenos como el Metharrizium anisopliae y Beauveria bassiana. La finalidad del presente estudio fue observar la capacidad patógena de dos cepas de Metarhizium anisopliae a dosis de 1X10 8 conidias/ml y 1.3X10 12 conidias/ml, y de una cepa de Beauveria bassiana a una dosis de 1.3X10 12 conidias/ml en unidades de producción en bovinos de doble propósito, en los municipios de Emiliano Zapata

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“…Capital letters denote significant differences between the weights of egg masses laid by infected ticks and those laid by the control group in each temperature treatment (P \ 0.05). Bar: mean ± SD on-mammal applications of conidia, higher environmental temperatures, along with the high temperatures found on the surface of the mammal may strongly influence the anti-ectoparasite activity of the fungi (Leemon et al 2008;Polar et al 2005). The ventral surface of a feeding tick is often in contact with the animal skin, which, in most cases, is hotter than the environment and less dependent on the ambient temperature.…”

Section: Discussionmentioning

confidence: 99%

“…This fungus very effectively controls several tick stages and also causes a variety of sub-lethal effects, such as reductions in molting and engorging rates, egg production and larval hatching (Gindin et al 2001;Kaaya and Hassan 2000;Kirkland et al 2004). However, the application of M. anisopliae against parasitic tick stages under field conditions, including on mammalian hosts, has yielded inconsistent results, which may be due to the effects of biotic and abiotic factors on the survival of the fungus and its ability to cause mycosis (Hall and Papierok 1982;Inglis et al 2001;Leemon et al 2008;Polar et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Thermal limitations of Metarhizium anisopliae efficacy: selection for application on warm-blooded vertebrates

et al. 2010

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Temperature is one of the main obstacles for on-host applications of entomopathogenic fungi for ectoparasite control. The effects of temperatures typical of the body surfaces of warm-blooded animals on the germination, growth and virulence of four strains of Metarhizium anisopliae toward engorged Rhipicephalus (Boophilus) annulatus females were evaluated. The M. anisopliae strains studied can be divided according to their thermal characteristics: (1) strains which germinate (90-100%), grow and infect ticks similarly at 25, 30 and 35°C; and (2) strains which recover their ability to germinate relatively quickly following a thermal shock (37 or 40°C for 6-48 h) before incubation at a favorable temperature. These latter strains could recover their infectivity after a short thermal shock (6 h at 37-40°C), but not after more prolonged exposure to these temperatures (48-72 h). These two thermal characteristics do not interact, but reflect the efficacy of strains used to control ectoparasites on warm-blooded vertebrates.

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Pen studies on the control of cattle tick (Rhipicephalus (Boophilus) microplus) with Metarhizium anisopliae (Sorokin)

Cited by 39 publications

References 15 publications

Endophytic and entomopathogenic strains of Beauveria sp to control the bovine tick Rhipicephalus (Boophilus) microplus

Endophytic and entomopathogenic strains of Beauveria sp to control the bovine tick Rhipicephalus (Boophilus) microplus

Control biológico de Rhicephalus (Boophilus) microplus con hongos entomopatógenos / Biological Control of Rhicephalus (Boophilus) microplus with Entomopathogenic Fungi

Thermal limitations of Metarhizium anisopliae efficacy: selection for application on warm-blooded vertebrates

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