Pemisahan Senyawa Aktif Fraksi Petroleum Eter dan Etil Asetat Hasil Hidrolisis Ekstrak Etanol Hydrilla verticillata dari Ranu Grati Pasuruan

Amalia, Suci; Fasya, Ahmad Ghanaim; Hasanah, Faiqotul; Yuliani, Dewi

doi:10.18860/al.v6i2.7014

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…Penelitian yang dilakukan oleh Amalia dkk. [12] dan Fasya dkk. [8] menyatakan bahwa fraksi petroleum eter hasil hidrolisis ekstrak etanol H. verticillata mengandung steroid.…”

Section: Pendahuluanunclassified

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Fasya

Purwantoro

Ulya

et al. 2020

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Hydrilla verticillata is one of Allah SWT creations that potential as an antioxidant. This study’s objectives were to determine antioxidant activity and identify steroid isolates of n-hexane fraction of H. verticillata. The steroid compounds were extracted using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using n-hexane. The n-hexane fraction was tested phytochemically and separated using thin-layer chromatography (TLC). The steroids compounds were identified using UV-Vis spectrophotometer, FTIR, and LC-MS/MS. The result showed that maceration extraction produced 5.14% yield, whereas n-hexane fraction produce 47.95% yield. Steroid separation through analytical TLC revealed that n-hexane: ethyl acetate (4:1) as the best eluant with 12 spots, while separation using preparative TLC yielded 19 spots. Steroid isolate from TLC preparative has antioxidant activity with EC50 of 5.109 ppm. Identification of steroid compounds using UV-Vis produced maximum wavelengths at 203.9 and 276 nm, while using FTIR indicated the presence of O-H group, geminal dimethyl, C=O, C=C, secondary C-OH, and =C-H (alkene) which might contain steroid compounds. The result of LC-MS/MS showed the presence of β-sitosterol. Hydrilla verticillata merupakan salah satu ciptaan Allah SWT yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan mengidentifikasi senyawa steroid pada isolat hasil pemisahan fraksi n-heksana H. verticillata. Senyawa steroid diekstraksi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi dengan n-heksana. Fraksi n-heksana hasil partisi diuji fitokimia dan dipisahkan senyawanya menggunakan KLT (Kromatografi Lapis Tipis). Identifikasi dilakukan menggunakan UV-Vis, FTIR, dan LC-MS/MS. Hasil penelitian menunjukkan ekstraksi maserasi menghasilkan rendemen 5,14%, sedangkan rendemen partisi n-heksana sebesar 47,95%. Pemisahan steroid menggunakan KLT analitik menunjukkan bahwa variasi eluen terbaik adalah n-heksana: etil asetat (4:1) dengan 12 noda, sedangkan pemisahan KLT preparatif menghasilkan 19 noda. Isolat steroid hasil KLT preparatif memiliki aktivitas antioksidan dengan EC50 5,109 ppm. Identifikasi senyawa steroid menggunakan UV-Vis menunjukkan bahwa panjang gelombang maksimum sebesar 203,9 dan 276 nm, sedangkan untuk identifikasi isolat steroid menggunakan FTIR menunjukkan gugus fungsi O-H, geminal dimetil, C=O, C=C, C-OH sekunder dan =C-H (alkena) yang diduga merupakan senyawa steroid. Hasil LC-MS/MS menunjukkan adanya senyawa steroid β-sitosterol.

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“…Penelitian yang dilakukan oleh Amalia dkk. [12] dan Fasya dkk. [8] menyatakan bahwa fraksi petroleum eter hasil hidrolisis ekstrak etanol H. verticillata mengandung steroid.…”

Section: Pendahuluanunclassified

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Fasya

Purwantoro

Ulya

et al. 2020

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“…Bioactivity is strongly influenced by the active compounds. The steroid compounds of the n-hexane fraction of H. verticillata are β-sitosterol, stigmasterol, campesterol, fucosterol, and cholesterol [20]. The n-hexane fraction of H. verticillata has antioxidant activity with an EC 50 value of 30.862 ppm [21].…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking of Selected Phytosterol from Hydrilla verticillata on Estrogen Receptors as Candidates of Anti-breast Cancer

Fasya¹,

Warsito²,

Iftitah³

et al. 2023

Advances in Engineering Research

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Hydrilla verticillata is an aquatic plant that contains various secondary metabolites, such as triterpenoids and steroids. Several steroid compounds contained in Hydrilla verticillata are β-sitosterol, stigmasterol, fucosterol, campesterol, and cholesterol. Steroid compounds have the potential as candidates for breast cancer compounds. The important factor in inhibition and invasion of breast cancer cells are Estrogen receptors. The estrogen receptor complexed with genistein is often the target model for drug action. The docking of phytosterol compounds in Hydrilla verticillata to estrogen receptors has been carried out to test the docking energy associated with the affinity and type of binding between the ligand and the receptor. Five steroid compounds Hydrilla verticillata which have the potential to inhibit the growth of breast cancer cells as well as genistein as comparisons were taken from the PubChem database. Molecular docking of these compounds to estrogen-α and estrogen-β receptors was carried out using software such as RSCB Protein Data Bank, PyrX Virtual Screening Tool, and BIOVIA Discovery Studio Visualizer. The results showed that β-sitosterol, stigmasterol, fucosterol, campesterol, and cholesterol had binding affinities of −7.3, −8.1, − 7.7, −8.1 and −7.6 kcal/mol towards 1X7R estrogen-α receptor and −6.8, −6.8, −7.5, −6.8 and −6.3 kcal/mol towards 1X7J estrogen-β receptor. While genistein as a control has a binding affinity of 7.7 kcal/mol towards 1X7R and 6.4 kcal/mol towards 1X7J. Some phytosterol compounds from Hydriylla verticillata have a better binding affinity than ginestein as a positive control, so it can be concluded that these compounds have the potential to anti-cancer.

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“…Hydrilla verticillata contain some secondary metabolite compounds such as flavonoids, steroids, and alkaloids [10]; phenols, glicosides, alkaloids and steroids [11]. Petroleum fractions from the ethanol extract of Hydrilla verticillata are positive contain steroids [12,6].…”

Section: Introductionmentioning

confidence: 99%

Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata

Fasya¹,

Amalia²,

Megawati³

et al. 2021

Advances in Social Science, Education and Humanities Research

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Hydrilla verticillata is one of the aquatic plants that contain some secondary metabolites, such as triterphenoids and steroids. The aim of this study was to determine the toxicity levels of steroids isolated from ethyl acetate fraction of Hydrilla verticillata separated by column chromatoghraphy. The biomass of Hydrilla verticillata was dried and powdered. Hydrilla verticillata powder is then extracted by maceration methods using ethanol solvent. The crude ethanol extracts were hydrolyzed using 2 N of HCl and partitioned using ethyl acetate solvent. The steroids compounds of ethyl acetate fraction were separated by Column Chromatography. The steroid isolates from Column Chromatography were identified by FTIR and UV-Vis spectrophotometer. The toxicity level of Column Chromatography steroid isolates was determined by BSLT method. The result of this research showed that extraction through maceration using ethanol produced 2.52 % yield, whereas the percent yield of the partition using ethyl acetate was 45.04 %. Separation by column chromatography resulted 3 steroids isolates B1, B2, and G1. The toxicity test showed that column chromatography steroids isolate from ethyl acetate fraction of Hydrilla verticillata has toxicity properties. The LC50 value of B1, B2 and G1 isolate was 14.58, 7.55 and 4.71 ppm, respectively.

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Pemisahan Senyawa Aktif Fraksi Petroleum Eter dan Etil Asetat Hasil Hidrolisis Ekstrak Etanol Hydrilla verticillata dari Ranu Grati Pasuruan

Cited by 3 publications

References 2 publications

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Molecular Docking of Selected Phytosterol from Hydrilla verticillata on Estrogen Receptors as Candidates of Anti-breast Cancer

Toxicity Test of Column Chromatography Steroids Isolates from Ethyl Acetate Fraction of Hydrilla verticillata

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