Pembuatan Pepton Dari Khamir Dengan Enzim Papain Untuk Media Pertumbuhan Bakteri

Fachraniah, Fachraniah; Fardiaz, Dedi; Idiyanti, Tami

doi:10.30811/jstr.v1i1.18

Cited by 3 publications

(3 citation statements)

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“…Media Peptones provide essential growth nutrients for organisms (Dede et al 2014). This peptone is also the main nitrogen source in commercial media for microbial growth which consists of a mixture of polypeptides, dipeptides, and amino acids which can be obtained from protein-containing materials through acid or enzymatic hydrolysis reactions (Fachraniah et al 2002) while istonic solutions contain Physiological NaCl is useful for the recovery of bacterial cells from vulnerable conditions to cells that are ready to grow.…”

Section: Resultsmentioning

confidence: 99%

Total Plate Number Test at 0.5 McFarland Standard in Escherichia coli Culture

Bhakti,

Porwanti,

Pribadi

et al. 2023

Bioma

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Estimation of the number of bacteria can be calculated quickly by comparing the turbidity of the test bacterial suspension against the McFarland standard. In the microbiology laboratory, McFarland standard of 0,5 is often used as reference. There are still few scientific references about the estimated enumeration of bacteria according to McFarland standards. The purpose of this study was to provide basic information on the estimated number of E.coli bacteria according to the McFarland standard of 0,5. The analytical method uses ISO SNI 16649-2: 2001 for the enumeration E.coli using TBX Agar Media with 10 replications to get a good average result. The result of E.coli enumeration with the 0,5 McFarland standard was 4,0 x 10 7 colonies/mL. Thus the approximate information on the bacteria enumeration could be done quickly.

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Section: Resultsmentioning

confidence: 99%

Total Plate Number Test at 0.5 McFarland Standard in Escherichia coli Culture

Bhakti,

Porwanti,

Pribadi

et al. 2023

Bioma

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“…Protein hydrolysate is a product of a mixture of polypeptides, dipeptides, and amino acids, can be produced from materials containing protein through acid hydrolysis or enzymatic reaction. Protein hydrolysate as the main nitrogen source in a commercial medium for bacterial growth is still imported at high prices (Fachraniah et al, 2002). Therefore, an alternative is needed to produce FPH from by-product as waste such viscera or fish meat at post rigor phase.…”

Section: Introductionmentioning

confidence: 99%

Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis

Saputra

Nurhayati

2016

ComTech

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Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be identified by analyzing the content of dissolved total nitrogen (NTT) compared with nitrogen total ingredient (NTB) in order to get the value of total soluble nitrogen/total nitrogen material (NTT/NTB). The hydrolysis processes were carried out in 0,26% (w/v) papain, 60 οC for 3 hours. The result showed that the specific activity of papain enzyme was about 3.28 U/mg. Solubility of FPH by comparing NTT/NTB was about 0.29% (fish meat) and 0.40% (fish viscera). Proximate test of protein content of fish meat was 18.34 ± 0.04 (g/100 g); while viscera was about 0.95±0.04 (g/100 g). The result indicated that product waste of fish carp had potential as a major of source of FPH.

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“…Sumber lain disampaikan oleh Strasdine dan Melville (1972); Beuchat (1974); Jassim et al (1988); Strom dan Raa (1993) yang melaporkan bahwa limbah ikan juga telah diteliti sebagai bahan dasar penghasil pepton. Pepton ini juga merupakan sumber nitrogen utama dalam media komersial untuk pertumbuhan mikroba yang terdiri dari campuran polipeptida, dipeptida, dan asam amino yang dapat diperoleh dari bahan yang mengandung protein melalui reaksi hidrolisis asam atau enzimatis (Fachraniah et al 2002).…”

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Produksi Dan Aplikasi Pepton Ikan Selar Untuk Media Pertumbuhan Bakteri

Saputra

Nurhayati

2014

JPHPI

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Pepton adalah hidrolisat protein yang larut dalam air dan tidak menggumpal jika dipanaskan. Tujuan daristudi ini adalah untuk: 1) mengevaluasi potensi ikan selar sebagai bahan baku pepton ikan, 2) mengoptimasipenggunaan enzim terpilih, 3) mengarakterisasi pepton ikan selar, 4) membandingkan mutu pepton ikanselar dengan pepton komersial sebagai media pertumbuhan bakteri. Proses hidrolisis ikan selar fase postrigor dengan penambahan enzim papain 0,26% (b/v) selama 6 jam. Analisis proksimat pepton ikan selar,menunjukkan kadar protein yang tinggi (74,17% basis basah) dan mengandung beberapa jenis asam aminoessensial, yaitu histidina, isoleusina, leusina, lisina, metionina, fenilalanina, treonina, valina, tirosina, danarginina. Uji karakteristik pepton ikan menunjukkan bahwa kelarutan pepton ikan post rigor yaitu 96,74%,dengan total nitrogen 11,86%, α-amino nitrogen 1,07 g/100 g, α-amino nitrogen per total nitrogen 9,02; dankadar garam 0,41%. Nilai optical density bakteri ditumbuhkan pada media yang menggunakan pepton ikanlebih tinggi dibandingkan pertumbuhan bakteri pada media yang menggunakan pepton komersial.Kata kunci: asam amino, enzim, hidrolisis, pepton, post rigor

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Pembuatan Pepton Dari Khamir Dengan Enzim Papain Untuk Media Pertumbuhan Bakteri

Cited by 3 publications

References 0 publications

Total Plate Number Test at 0.5 McFarland Standard in Escherichia coli Culture

Total Plate Number Test at 0.5 McFarland Standard in Escherichia coli Culture

Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis

Produksi Dan Aplikasi Pepton Ikan Selar Untuk Media Pertumbuhan Bakteri

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