The aim of this study is for knowing the effect of giving sarang semut extract in various dosage and time toward the anti-body of avian influenza H5N1 for the poultry, especially broiler chicken. This research is experimental method using RAL Factorial Design. Factor I is the addition of sarang semut extract amounted 5mgs/kg BB, 10 mgs/kg BB, 15 mgs/kg BB and Factor II is the length of giving sarang semut extract, namely: 3 days, 5 days, and 7 days. The comparing experiment factor as controlled variable is using AI vaccine and without AI vaccine. The data are analyzed using Analysis of Variance (ANOVA) continued by BNT 5% test. The result of serology test proving that adding sarang semut extract showing the significant difference in each experiment, but not showing the significant difference in the different time given. The result of serology test in this research shows that after giving AI vaccine (either experimental or control group) showing protective anti-body. The more increasing of the anti-body titer average and more decreasing CV can give the more optimal protection of H5N1 virus attack. The result of best average titer and CV recommended in this research is giving sarang semut extract by 10 mgs/kg BB dosage.