PEMANFAATAN FRAKSI KAYA ASAM LAURAT HASIL HIDROLISIS DARI ENDOSPERM KELAPA MENGGUNAKAN LIPASE ENDOGENEUS SEBAGAI PENGAWET SUSU KEDELAI KEMASAN (Utilization of High Lauric Fraction that Produced from Coconut Endosperm Using Lipase Endogenous as Preservation of Soybean Milk Packaging)

Abstract

ABSTRAKHasil penelitian sebelumnya menunjukkan bahwa fraksi kaya asam laurat hasil isolasi dari endosperm kelapa mampu menghambat bakteri patogen dan non patogen. Penelitian ini bertujuan mempelajari penambahan fraksi kaya asam laurat hasil hidrolisis dari endosperm kelapa terhadap daya simpan susu kedelai kemasan. Fraksi yang kaya asam laurat diisolasi dari santan kelapa kemudian fraksi tersebut diuji komposisi asam lemaknya menggunakan chromatografi gas (GC) dan selanjutnya digunakan sebagai bahan pengawet susu kedelai. Fraksi kaya asam laurat ditambahkan ke dalam susu kedelai dengan konsentrasi 0, 10, 15 dan 20%, kemudian disimpan selama 3 hari. Setiap hari dilakukan pengamatan hingga susu mengalami kerusakan. Hasil penelitian menunjukkan bahwa fraksi hasil isolasi dari santan kelapa mengandung asam laurat 50,45%, asam miristat 17,52%, asam palmitat 7,02%, asam kaprat 6,46%, asam kaprilat 5,52%, asam linoleat 5,12%, asam oleat 1,89% dan asam kaproat 0,11%. Penambahan fraksi kaya asam laurat sebesar 20% mampu mengawetkan susu kedelai selama 2 hari dengan total mikroba 1,00 x 10 4 cfu/ml, asam lemak bebas 0,12 m mol/ml, pH 5,05 dan skor aroma 4 (menyukai). Kata kunci: Kelapa, asam laurat, susu kedelai, penyimpanan ABSTRACTResults of previous studies show that the high lauric fraction isolated from coconut endosperm is able to inhibit pathogenic and non-pathogenic bacteria. This research aims to study the addition of high lauric fraction that hydrolysed of coconut endosperm of the storability of soy milk packaging. High lauric fraction isolated from coconut milk, then the fraction analized of the fatty acid composition with gas chromatography (GC) and then used as a preservative soy milk. The fraction is added to the soy milk with concentrations of 0, 10, 15 and 20%, then stored for 3 days. Every day is observed until soy milk damaged. The results showed that the fraction isolated from coconut milk contains 50.45% lauric acid, 17.52% myristic acid, 7.02% palmitic acid, 6.46% capric acid, 5.52% caprylic acid, 5.12% linoleic acid, 1.89% oleic acid, and 0.11% caproic acid. The addition of lauric acid-rich fraction of 20% were able to preserve soy milk for 2 days with a total microbe 1.00 x 104 cfu/ml, free fatty acids 0.12 m mol/ml, pH 5.05 and a balanced aroma 4 (nice).

The Activity of Lipase From Jatropha Seed (Jatropha Curcas L.) And Its Application On Hydrolysis of Castor Oil In Organic Solvent

“…Different from the case of metal ions, Na⁺ and Mg²⁺ can act as inhibitors at a concentration of 10 mM, reducing lipase activity to 63.048% and 66.743%. This condition is close to research by Sui et al (2012) on coconut-endosperm , in which the lipase enzyme activity increased to 64% after adding 5 mM of Na⁺ [8]. Another study by Yesiloglu and Baskurt (2013) explained that the Mg²⁺ metal ion could very strongly inhibit the lipase activity of almond seeds [12].…”

“…Previous research has been reported by Khairunnisa et al (2021) on camped seed sprouts, in which K⁺ and Mg²⁺ were act as activators that can increase lipase activity to 101%, 680%, and 131.09% [7]. Sui et al (2012) research on coconut endosperm using addition of Na⁺ as an inhibitor work to 64% [8]. Another study by Yesiloglu and Baskurt (2013) explained that the Mg²⁺ could strongly inhibit the lipase activity of almond seeds [9].…”

Potential Biodegradable Sticker Based on Carrageenaan and Chitosan with Addition of Lauric Acid: A Mini Review

“…Coconut oil contains approximately 51-53% lauric acid, which can be separated from the oil through hydrolysis with the assistance of water under high temperature and pressure. After hydrolysis, coconut oil is transformed into free fatty acids (including lauric acid) and glycerol [29].…”

Pelatihan dan Pendampingan Usaha Kreatif VCO Desa Palingi Timur Konawe Kepulauan

“…Lucida et al (2008) menyebutkan bahwa VCO mengandung 92% asam lemak jenuh, di mana 48%-53% mengandung asam laurat. Pujiati (2012) diacu dalam Sui et al (2017) menjelaskan bahwa asam laurat merupakan asam lemak jenuh yang biasa disebut Medium Chain Fatty Acid (MCFA) dapat melarutkan membran virus dan mengganggu kekebalan virus sehingga virus tersebut berada dalam kondisi inaktivasi. Selanjutnya dijelaskan bahwa asam laurat dalam tubuh manusia akan diubah menjadi monolaurin, yaitu senyawa monogliserida yang bersifat antivirus, antibakteri, dan antiprotozoa.…”