PEGylated PEI-based biodegradable polymers as non-viral gene vectors

Huang, Farong; Wang, Huiyuan; Cao, Li; Wang, Huafen; Sun, Yunxia; Feng, Jie; Zhang, Xian‐Zheng; Zhuo, Ren‐Xi

doi:10.1016/j.actbio.2010.06.016

Cited by 73 publications

(48 citation statements)

References 26 publications

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“…Targeted gene delivery can be done by attaching different types of functionalized groups such as carboxyl groups, amines, biotin, streptavidin, antibodies, and polyethyleneimine (particularly in vitro uses) to shell or matrix. The recent research showed that transfection time is significantly reduced for magnetic nanoparticles in comparison to that for non-viral agents and that magnetic nanoparticles have been used to successfully deliver small interfering RNA and antisense oligonucleotides under in vitro and in vivo conditions [29,52]. Recently, magnetic calcium phosphate nano-formulations have been used for transfection of DNA [53].…”

Section: Reviewmentioning

confidence: 99%

A sight on the current nanoparticle-based gene delivery vectors

2014

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Nowadays, gene delivery for therapeutic objects is considered one of the most promising strategies to cure both the genetic and acquired diseases of human. The design of efficient gene delivery vectors possessing the high transfection efficiencies and low cytotoxicity is considered the major challenge for delivering a target gene to specific tissues or cells. On this base, the investigations on non-viral gene vectors with the ability to overcome physiological barriers are increasing. Among the non-viral vectors, nanoparticles showed remarkable properties regarding gene delivery such as the ability to target the specific tissue or cells, protect target gene against nuclease degradation, improve DNA stability, and increase the transformation efficiency or safety. This review attempts to represent a current nanoparticle based on its lipid, polymer, hybrid, and inorganic properties. Among them, hybrids, as efficient vectors, are utilized in gene delivery in terms of materials (synthetic or natural), design, and in vitro/in vivo transformation efficiency.

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Section: Reviewmentioning

confidence: 99%

A sight on the current nanoparticle-based gene delivery vectors

2014

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“…Life Sci. 2015, 15,[489][490][491][492][493][494][495][496][497][498] www.biotecvisions.com Lower N/P ratio as much as possible while possessing residual positive charge Polyplex is less than 150 nm in size to enable cellular internalization; no aggregation [31,34] Polycation solubility Add water soluble moieties, adjust degree of acetylation and/or molecular weight Soluble in physiological fluids at appropriate temperature and pH [22,23,35,43,44] Polycation structural flexibility Application of long oligomethylene spacers Improvement in transfection efficiency [47] Polyplex colloidal stability PEGylation or other similar colloidal-stabilizing agents Improved structural and storage stability, no aggregation, increased circulation time, lower cytotoxicity [49][50][51][52] transfection efficiencies and cytotoxicity levels that are clinically acceptable.…”

Section: Types Of Polycations and Improvement Of Their Propertiesmentioning

confidence: 99%

Polycation‐mediated gene delivery: Challenges and considerations for the process of plasmid DNA transfection

Modra

Dai

Zhang

et al. 2015

Engineering in Life Sciences

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The process of gene delivery has increasingly become a focus in biomedical research due to the growing demand by an ageing global population for targeted therapies of genetic related diseases, such as cancer, immunodeficiencies, and cystic fibrosis. In light of the safety issues that viral vectors still face in progressing through clinical trials, polycations have alternatively attracted keen attention, due to their lower safety risks and ability to interact with cells more effectively and with more stability, compared with other chemically designed nonviral vectors. In this review, a reflection of the literature pertaining to various types of polycations designed and optimized is presented, including the obstacles they face in facilitating plasmid DNA transfection. In order to design new polycations or optimize current ones that will be successful—both economically and for use in future clinical therapies—further in vivo research is needed to fully elucidate the mechanisms of each stage in the delivery process.

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“…In addition, drug-loaded PIC could release the drug into the cytosol from PIC. 15,[26][27][28] characterization of PIc PEG-PLA-PEI itself did not form aggregates due to the hydrophilicity of PEI and PEG compared with the hydrophobicity of PLA, and was soluble in aqueous solution. However, the complexes formed with cationic PEI and anionic P(Asp) by electrostatic interaction possessed a hydrophobic compartment due to neutralization, which could provide a driving force to form micelle-like aggregation.…”

Section: Titration Of the Synthesized Peg-pla-peimentioning

confidence: 99%

“…14,15 The PEG-PEI block copolymers exhibited improved solubility even under chargeneutralized conditions. However, the PIC system with this type of double hydrophilic block copolymer and oppositely charged molecules can be dissociated in in vivo conditions by other counterions, and showed a lower cell transfection of the therapeutic agents due to the lack of self-assembling aggregation force.…”

mentioning

confidence: 99%

Development of a robust pH-sensitive polyelectrolyte ionomer complex for anticancer nanocarriers

Lim¹,

Youn²,

Lee³

et al. 2016

IJN

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A polyelectrolyte ionomer complex (PIC) composed of cationic and anionic polymers was developed for nanomedical applications. Here, a poly(ethylene glycol)–poly(lactic acid)–poly(ethylene imine) triblock copolymer (PEG–PLA–PEI) and a poly(aspartic acid) (P[Asp]) homopolymer were synthesized. These polyelectrolytes formed stable aggregates through electrostatic interactions between the cationic PEI and the anionic P(Asp) blocks. In particular, the addition of a hydrophobic PLA and a hydrophilic PEG to triblock copolyelectrolytes provided colloidal aggregation stability by forming a tight hydrophobic core and steric hindrance on the surface of PIC, respectively. The PIC showed different particle sizes and zeta potentials depending on the ratio of cationic PEI and anionic P(Asp) blocks (C/A ratio). The doxorubicin (dox)-loaded PIC, prepared with a C/A ratio of 8, demonstrated pH-dependent behavior by the deprotonation/protonation of polyelectrolyte blocks. The drug release and the cytotoxicity of the dox-loaded PIC (C/A ratio: 8) increased under acidic conditions compared with physiological pH, due to the destabilization of the formation of the electrostatic core. In vivo animal imaging revealed that the prepared PIC accumulated at the targeted tumor site for 24 hours. Therefore, the prepared pH-sensitive PIC could have considerable potential as a nanomedicinal platform for anticancer therapy.

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PEGylated PEI-based biodegradable polymers as non-viral gene vectors

Cited by 73 publications

References 26 publications

A sight on the current nanoparticle-based gene delivery vectors

A sight on the current nanoparticle-based gene delivery vectors

Polycation‐mediated gene delivery: Challenges and considerations for the process of plasmid DNA transfection

Development of a robust pH-sensitive polyelectrolyte ionomer complex for anticancer nanocarriers

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