Pegylated Interferon Versus Standard Interferon and Silymarin in Treatment of Liver Fibrosis Induced by Chronic Carbon Tetrachloride in Rats

Mostafa, Rasha E.; Zaki, Hala F.; Sleem, Amany A.; Abdel-Sala, Omar M.; Morsy, Fatma A.; Kenawy, Sanaa A.

doi:10.5567/pharmacologia.2013.208.217

Cited by 5 publications

(7 citation statements)

References 10 publications

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“…Therefore, silymarin was chosen as a comparator. In line with our findings, a study by Mostafa et al reported that the administration of silymarin 100 mg/kg/day decreased ALP activity significantly compared with the fibrosis group [31]. Studies by Tsai et al and Amin et al had also shown a decrease in ALP enzyme activity after administration of silymarin 200 mg/kg [24], [26].…”

Section: Discussionsupporting

confidence: 91%

Antifibrotic Activity of Phaleria macrocarpa Extract in Rat Liver-fibrosis Model: Focus on Oxidative Stress Markers, TGF-β1 and MMP-13

Wardhani

Sundari

Tjandrawinata³

et al. 2020

Open Access Maced J Med Sci

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AIM: This study was aimed to determine the antifibrotic activity of Phaleria macrocarpa (PM) extract in liver fibrosis (LF) and its possible mechanism in the rat model. METHODS: Sprague Dawley male rats were injected with 2 mL/kg BW of carbon tetrachloride intraperitoneally twice a week for 2 weeks, followed by 1 mL/kg BW for 6 weeks. Afterward, the treatments began from the 3rd week: Silymarin 100 mg/kg BW/day, standardized PM extract (Proliverenol) 75 or 150 mg/kg BW/day orally. Rats were sacrificed in the 8th week. Blood and liver were collected to analyze liver function, liver damage and fibrosis marker, oxidative stress markers, pro-fibrogenic cytokine, and antifibrotic marker. RESULTS: Our study showed that the treatment of silymarin and PM resulted in the normalized activity of liver function, followed by the amelioration of oxidative stress, demonstrated by the decreased malondialdehyde levels and an increased ratio of glutathione and glutathione disulfide. All markers examined showed that PM extract has antioxidant activity due to decreased hepatic stellate cell activation. We also found a decrease in tumor growth factors-β1 and protein expressions of matrix metalloproteinases-13 in all treatment groups compared to the carbon tetrachloride group. There were tendencies of the decreased fibrotic area following improvements of biochemical parameters. CONCLUSION: PM extracts ameliorate carbon tetrachloride-induced LF. The proposed mechanism is by overcoming oxidative stress and regulating pro-fibrogenic cytokine and antifibrotic markers.

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Section: Discussionsupporting

confidence: 91%

Antifibrotic Activity of Phaleria macrocarpa Extract in Rat Liver-fibrosis Model: Focus on Oxidative Stress Markers, TGF-β1 and MMP-13

Wardhani

Sundari

Tjandrawinata³

et al. 2020

Open Access Maced J Med Sci

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“…Silymarin administration significantly declined liver Hyp content induced by TAA -treatment and there were significant improvement in the histopathological and histochemical hepatic architecture. It was found that silymarin inhibit stellate hepatocytes transformation to myofibroblasts, which is responsible for collagen deposition in CCl4induced hepatic fibrosis [56,57].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Grape Seed Extract and/or Silymarin Against Thioacetamide-induced Hepatic Fibrosis in Rats

Nada¹,

Gowifel²,

Eldenshary³

et al. 2015

J Liver

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The aim of the present study was designed to evaluate the hepatoprotective and antioxidant potentials of GSE (100 and 200 mg/kg) and/or silymarin against TAA-induced liver fibrosis in rats. This study was designed to investigate the protective effect of grape seed extract (GSE) and/or silymarin against thioacetamide (TAA)-induced hepatic fibrosis in Sprague-Dawley rats. Mature male Sprague-Dawley rats were divided into 7 equal groups (8 rats each) and treated as follows: Group 1, kept as control group and orally given saline; groups 2-7 were injected intraperitoneally (i.p.) with TAA (100 mg/Kg) twice weekly for 6 weeks to induce hepatic fibrosis. Group 2, kept as control positive; groups 3-5 were administered daily oral doses of silymarin (50 mg/kg), GSE (100 mg/kg) and GSE (200 mg/kg), respectively. While groups 6-7 were administered combined treatments of silymarin and GSE (100 mg/kg) or GSE (200 mg/kg), respectively. Our results indicated that TAA caused significant elevation of hydroxyproline (Hyp), malondialdehyde (MDA) and nitric oxide (NO) contents in liver homogenate and increased serum levels of: aminotransferases (AST and ALT), alkaline phosphatase (ALP) and total bilirubin. While, TAA-treatment alone significantly decreased serum total protein and reduced glutathione (GSH) content in liver homogenate. Administration of GSE (100 and 200 mg/kg) and/or silymarin attenuated TAA-induced hepatic fibrosis, improved enzymes and reduced the oxidative stress in dose dependant manner Histopathological study showed disruption of the hepatic architecture and collagen fibers deposition in the portal tract of TAA-injected group. Concomitant treatment with GSE (100 and 200 mg/kg) and/or silymarin significantly improved histopathological structure of liver tissue in variable degrees. In conclusion, the combined effect of GSE (200 mg/kg) with silymarin (50 mg/kg) had powerful hepatoprotective effect than any other studied doses.

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“…Hydroxyproline quantification is considered the most common method for evaluating tissue fibrosis/collagen deposition [50]. Histopathologically, the decrease in hydroxyproline content approximately paralleled decreases in the fibrosis stage [32].…”

Section: Introductionmentioning

confidence: 99%

Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats

et al. 2015

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Pegylated Interferon Versus Standard Interferon and Silymarin in Treatment of Liver Fibrosis Induced by Chronic Carbon Tetrachloride in Rats

Cited by 5 publications

References 10 publications

Antifibrotic Activity of Phaleria macrocarpa Extract in Rat Liver-fibrosis Model: Focus on Oxidative Stress Markers, TGF-β1 and MMP-13

Antifibrotic Activity of Phaleria macrocarpa Extract in Rat Liver-fibrosis Model: Focus on Oxidative Stress Markers, TGF-β1 and MMP-13

Protective Effect of Grape Seed Extract and/or Silymarin Against Thioacetamide-induced Hepatic Fibrosis in Rats

Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats

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