Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism

A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

“…For more information on the expected outcomes of other metabolic parameters not described in this protocol, please refer to Zhang et al. (2022a) .…”

“…It has been studied in numerous types of arginine auxotrophic cancer, including hepatocellular carcinoma ( Abou-Alfa et al., 2018 ; Patil et al., 2016 ). In addition, we recently demonstrated that ADI-PEG 20 also improves insulin and glucose tolerance in genetically obese mice, in part, by driving systemic autophagic flux ( Zhang et al., 2022a , 2022b ). Therefore, we describe in this protocol the use of ADI-PEG 20 to treat metabolic disease in diet-induced and genetically obese mouse models, and subsequent quantification of key outcome measures ( Zhang et al., 2022a ).…”

“…Statistical significance was determined using unpaired two-tailed Student’s t -test in (A), (B), and (C). Data presented in (B) is reprinted with permission from Zhang et al., 2022a , Zhang et al., 2022b . …”

“…We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a , 2022b) .…”

“…For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a , 2022b) .…”