PEG-Functionalized Magnetite Nanoparticles for Modulation of Microbial Biofilms on Voice Prosthesis

Abstract

This study reports the fabrication of nanostructured coatings based on magnetite, polyethyleneglycol, and biologically active molecule (polymyxin B-PM) for producing biofilm-resistant surfaces (voice prosthesis). Magnetite nanoparticles (MNPs) have been synthesized and functionalized using a co-precipitation method and were further deposited into thin coatings using the matrix-assisted pulsed laser evaporation (MAPLE) technique. The obtained nanoparticles and coatings were characterized by X-ray diffraction (XRD), thermogravimetric analysis with differential scanning calorimetry (TGA-DSC), scanning electron microscopy (SEM), transmission electron microscopy with selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FT-IR), and infrared microscopy (IRM). Their antibiofilm activity was tested against relevant Staphylococcus aureus and Pseudomonas aeruginosa bacterial strains. The Fe3O4@PEG/PM surface of modified voice prosthesis sections reduced the number of CFU/mL up to four orders of magnitude in the case of S. aureus biofilm. A more significant inhibitory effect is noticed in the case of P. aeruginosa up to five folds. These results highlight the importance of new Fe3O4@PEG/PM in the biomedical field.

Performance of Graphene/Polydimethylsiloxane Surfaces against S. aureus and P. aeruginosa Single- and Dual-Species Biofilms

“…Although the studied pristine GNP-based materials combined good performance with the simplicity of preparation compared to functionalized composites, graphene functionalization with bioactive molecules and/or polymers may improve its antimicrobial performance. This will be explored in the future, as the percentages of biofilm reduction achieved here were lower than those reported in the literature for these particular bacteria in other coating systems for implantable medical devices [ 95 ]. For example, using nanocoatings based on magnetite, polyethyleneglycol, and a biologically active molecule (polymyxin B-PM), Caciandone and colleagues [ 95 ] were able to reduce S. aureus and P. aeruginosa culturable cells of up to four and five orders of magnitude, respectively.…”

“…This will be explored in the future, as the percentages of biofilm reduction achieved here were lower than those reported in the literature for these particular bacteria in other coating systems for implantable medical devices [ 95 ]. For example, using nanocoatings based on magnetite, polyethyleneglycol, and a biologically active molecule (polymyxin B-PM), Caciandone and colleagues [ 95 ] were able to reduce S. aureus and P. aeruginosa culturable cells of up to four and five orders of magnitude, respectively.…”

Dextran-Coated Iron Oxide Nanoparticles Loaded with Curcumin for Antimicrobial Therapies

“…The protocols were performed according to previous antimicrobial studies [ 30 , 31 ]. The antimicrobial effect was assessed for the pristine iron oxide controls, dextran-coated, and curcumin-loaded dextran-coated iron oxide nanoparticles.…”

Design, Characterization, and Antibacterial Performance of MAPLE-Deposited Coatings of Magnesium Phosphate-Containing Silver Nanoparticles in Biocompatible Concentrations

“…aeruginosa. A cellular suspension was obtained according to specific protocols to quantify the number of units that can form bacterial colonies (CFU/mL) [ 74 ].…”