Peer Reviewed: Life and Death of a Single Enzyme Molecule

Craig, Douglas B.; Arriaga, Edgar A.; Wong, Jerome C. Y.; Lu, Hui; Dovichi, and Norman J.

doi:10.1021/ac9817164

Cited by 37 publications

(27 citation statements)

References 11 publications

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“…A variability (sensitivity, selectivity) of LIF detection is given by employing different lasers fthe common argon laser (458 and 488 nm), He-Cd laser (440 nm), blue diode laser (420 nm), frequency-doubled Ar-ion laser (257 nm), frequency-doubled Kr-ion laser (284 nm)g [7,[288][289][290]. To overcome problems associated with on-column detection such as broad-band luminescence background and poor S/N, Rayleigh and Raman light scattering from the capillary, several techniques based on the spatial or spectral separation of the fluorescence signal from the high background signal have been proposed (the best results have been reached with a sheath flow cuvette incorporated at the end of the capillary, performing an end-column LIF) [291][292][293][294].…”

Section: Fluorescence Detectionmentioning

confidence: 99%

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

Mikuš

Maráková

2009

Electrophoresis

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An analysis of recent trends indicates that CE can show real advantages over chromatographic methods in ultratrace enantioselective determination of biologically active compounds in complex biological matrices. It is due to high separation efficiency and many applicable in-capillary electromigration effects in CE (countercurrent migration, stacking effects) enhancing significantly (enantio)separability and enabling effective sample preparation (preconcentration, purification, analyte derivatization). Other possible on-line combinations of CE, such as column coupled CE-CE techniques and implementation of nonelectrophoretic techniques (extraction, membrane filtration, flow injection) into CE, offer additional approaches for highly effective sample preparation and separation. CE matured to a highly flexible and compatible technique enabling its hyphenation with powerful detection systems allowing extremely sensitive detection (e.g. LIF) and/or structural characterization of analytes (e.g. MS). Within the last decade, more as well as less conventional analytical on-line approaches have been effectively utilized in this field and their practical potentialities are demonstrated on many new application examples in this article. Here, three basic areas of (enantioselective) drug bioanalysis are highlighted and supported by a brief theoretical description of each individual approach in a compact review structure (to create integrated view on the topic), including (i) progressive enantioseparation approaches and new enantioselective agents, (ii) in-capillary sample preparation (preconcentration, purification, derivatization), and (iii) detection possibilities related to enhanced sensitivity and structural characterization.

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Section: Fluorescence Detectionmentioning

confidence: 99%

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

Mikuš

Maráková

2009

Electrophoresis

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“…Swaile and Sepaniak [45] were the first of demonstrate native fluorescence with on-column detection using UV excitation (frequency-doubled Ar-ion laser, 257 nm). They were capable of detection conalbumin at a [47,48].…”

Section: Direct On-column Detectionmentioning

confidence: 99%

Detection in capillary electrophoresis

2000

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“…Instead, Yeung's group at Iowa State University and our group at the University of Alberta developed a capillary-based method for monitoring the activity of single enzyme molecules [17][18][19][20]. Yeung studied lactate dehydrogenase from rabbit muscle while we studied alkaline phosphatase isolated from calf intestine.…”

Section: Single-molecule Enzymologymentioning

confidence: 99%