Peculiarities of studying an isolated neuron by the method of laser interference microscopy

The area, thickness, and volume of erythrocytes of different types (discocytes, stomatocytes, and echinocytes) from normal subjects and coronary patients were studied by laser interference microscopy. Increase of pH value leading to the stomatocyte-discocyte-echinocyte transformations resulted in a slight decrease of cell volume. In coronary patients, erythrocyte had larger area and volume and exhibited increased aggregation capacity compared to erythrocytes from controls. The results recommend laser interference microscopy as an adequate method for erythrocyte evaluation in laboratory diagnostic measurements.

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“…Cell height was calculated by the formula [7]. The coefficient of erythrocyte solution refraction was taken for 1.39 [2].…”

Section: Methodsmentioning

confidence: 99%

“…Laser interference microscopy (LIM) is used for studies of reflecting, transparent, and semitransparent biological objects, for example, living cells and their organelles [1,4,7,9,13]. Alteration of erythrocyte shape and status is a diagnostic marker of various diseases [5,6].…”

mentioning

confidence: 99%

Evaluation of erythrocyte shape and status by laser interference microscopy

et al. 2008

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“…Superposition of the two beams results in the fringe pattern on the detector due to interference between the object and reference beams. Thus LIM measures the optical path difference of the two beams (Yusipovich et al, 2006).…”

Section: Lim Theorymentioning

confidence: 99%

“…There are a lot of various type of cell were estimated using different methods of interference microscopy: erythrocytes (see e.g. Popescu et al, 2005;Popescu et al, 2006;Rappaz et al, 2007;Rappaz et al, 2008;Rappaz et al, 2009;Yusipovich, I et al, 2009;Yusipovich et al, 2011), fibroblasts (Dunn & Zicha, 1995), lymphocytes (Tychinsky et al, 2012), neurons (Rappaz et al, 2005;Yusipovich et al, 2006), nerve fibers (see e.g. Bibineyshvili et al, 2013;Brazhe et al, 2008;LaPorta & Kleinfeld, 2012;Maksimov et al, 2001), mast cells (Bondarenko et al, 2013), any microbiological objects (Tychinskii et al, 2007;Yusipovich et al, 2012;Zhurina et al, 2013) and others.…”

Section: Introductionmentioning

confidence: 99%

Application of Phase Images for Estimation of Peritoneal Macrophages State

Yusipovich¹,

Baizhumanov²,

Казакова³

et al. 2015

IJB

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Procedure based on phase images obtained by the method of Laser Interference Microscopy (LIM) for detection of number non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro was provided. The phase images provide quantitative estimates of the optical path difference (OPD) in each point of object. The OPD varies with cell thickness and concentration of cellular substances. Therefore, it provides useful information on cell structure, localization of subcellular structures, local concentrations of substances and dry mass of cell. In these work we propose a new assay for estimation a number of non-activated, activated and dead isolated peritoneal macrophages in a sample in vitro based on cell phase images. The results were compared with phagocytic index, which was evaluated using latex beads, and the percentage of cells with damaged membranes (dead cells). It was shown that with increasing time after the isolation, the percentage of dead macrophages estimated by LIM was similar to that of the cells with damaged membranes. However, the percentage of activated cells calculated using latex beads exceeded the percentage calculated using the phase images significantly.

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“…At present, phase images have been obtained for a variety of cell types, organelles and even whole organisms: neurons Rappaz et al 2005;Sosnovtseva et al 2005;Brazhe et al 2006;Yusipovich et al 2006), erythrocytes (Popescu et al 2004(Popescu et al , 2005Brazhe et al 2006), mast cells , HeLa cells (Popescu et al 2004;Choi et al 2007), epithelial kidney cells (Park et al 2006), chloroplasts (Tychinsky et al 2004) and the nematode Caenorhabditis elegans (Choi et al 2007). Studies of intracellular dynamics by means of interference microscopy are less numerous.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive study of nerve fibres using laser interference microscopy

Brazhe

Rodionova

et al. 2008

Phil. Trans. R. Soc. A.

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This paper presents the results of a laser interference microscopy study of the morphology and dynamical properties of myelinated nerve fibres. We describe the principles of operation of the phase-modulated laser interference microscope and show how this novel technique allows us to obtain information non-invasively about the internal structure of different regions of a nerve fibre. We also analyse the temporal variations in the internal optical properties in order to detect the rhythmic activity in the nerve fibre at different time scales and to shed light on the underlying biological processes. We observe pronounced frequencies in the dynamics of the optical properties and suggest that the oscillatory modes have similar origin in different regions, but different strengths and mutual modulation properties.

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Peculiarities of studying an isolated neuron by the method of laser interference microscopy

Cited by 20 publications

References 5 publications

Evaluation of erythrocyte shape and status by laser interference microscopy

Evaluation of erythrocyte shape and status by laser interference microscopy

Application of Phase Images for Estimation of Peritoneal Macrophages State

Non-invasive study of nerve fibres using laser interference microscopy

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