Peculiar properties of some components in a plant cell vacuole morphological structure revealed by confocal microscopy

Nurminsky, V. N.; Ракевич, А. Л.; Мартынович, Е. Ф.; Озолина, Н. В.; Nesterkina, I. S.; Колесникова, Е. В.; Pilipchenko, A. A.; Salyaev, R. K.; Chernyshov, Michael Yu.

doi:10.1134/s1990519x15050090

Cited by 2 publications

(3 citation statements)

References 29 publications

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“…For this purpose, the laurdan fluorescence GP index was used, which can vary from -1 to +1. Its negative values correspond to lower microviscosity (higherfluidity) of the cell membrane (Nurminsky et al, 2015) (see Materials and Methods).…”

Section: генетика микроорганизмов / Microbial Geneticsmentioning

confidence: 99%

Effect of colchicine on physiological and biochemical properties of <i>Rhodococcus qingshengii</i>

et al. 2022

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The genus Rhodococcus includes polymorphic non-spore-forming gram-positive bacteria belonging to the class Actinobacteria. Together with Mycobacterium and Corynebacterium, Rhodococcus belongs to the Mycolata group. Due to their relatively high growth rate and ability to form biof ilms, Rhodococcus are a convenient model for studying the effect of biologically active compounds on pathogenic Mycolata. Colchicine was previously found to reduce biof ilm formation by P. carotovorum VKM B-1247 and R. qingshengii VKM Ac-2784D. To understand the mechanism of action of this alkaloid on the bacterial cell, we have studied the change in the fatty acid composition and microviscosity of the R. qingshengii VKM Ac-2784D membrane. Nystatin, which is known to reduce membrane microviscosity, is used as a positive control. It has been found that colchicine at concentrations of 0.01 and 0.03 g/l and nystatin (0.03 g/l) have no signif icant effect on the survival of R. qingshengii VKM Ac-2784D cultivated in a buffered saline solution with 0.5 % glucose (GBSS). However, colchicine (0.03 g/l) signif icantly inhibits biof ilm formation. Rhodococcus cells cultivated for 24 hours in GBSS with colchicine acquire a rounded shape. Colchicine at 0.01 g/l concentration increases C16:1(n-7), C17:0, C20:1(n-9) and C21:0 fatty acids. The microviscosity of the membrane of individual cells was distributed from the lowest to the highest values of the generalized laurdan f luorescence polarization index (GP), which indicates a variety of adaptive responses to this alkaloid. At a higher concentration of colchicine (0.03 g/l) in the membranes of R. qingshengii VKM Ac-2784D cells, the content of saturated fatty acids increases and the content of branched fatty acids decreases. This contributes to an increase in membrane microviscosity, which is conf irmed by the data on the GP fluorescence of laurdan. All of the above indicates that colchicine induces a rearrangement of the Rhodococcus cell membrane, probably in the direction of increasing its microviscosity. This may be one of the reasons for the negative effect of colchicine on the formation of R. qingshengii VKM Ac-2784D biof ilms.

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Section: генетика микроорганизмов / Microbial Geneticsmentioning

confidence: 99%

Effect of colchicine on physiological and biochemical properties of <i>Rhodococcus qingshengii</i>

et al. 2022

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“…Furthermore, V-H + -ATPase activity of plant vacuoles is probably similar to that in animal organelles, and it has been investigated earlier, for example, in the aspect of stress [9] [18]. It has been discovered that there are numerous cytoplasmic strands starting in the vacuolar membrane and going beyond its limits [17] [19].…”

Section: Introduction and Problem Statementmentioning

confidence: 99%

“…It is known, for example, that vacuolar membranes are mediators of transmembrane transport. Some specialists register vacuolar membrane invaginations accompanied with separation of vesicles [15][16][17]…”

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Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy

Chernyshov¹,

Nurminsky²,

Озолина³

2017

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Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed. Keywords

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Peculiar properties of some components in a plant cell vacuole morphological structure revealed by confocal microscopy

Cited by 2 publications

References 29 publications

Effect of colchicine on physiological and biochemical properties of <i>Rhodococcus qingshengii</i>

Effect of colchicine on physiological and biochemical properties of <i>Rhodococcus qingshengii</i>

Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy

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