The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid-protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and the insolubility of these microdomains in nonionic detergents at low temperatures is proven. The elucidation of tonoplast microdomains has been based on results obtained with the aid of high-speed centrifuging in the sucrose gradient. The experiments have shown that tonoplast microdomains are rich in sphingolipids, free sterols and saturated fatty acids (such a lipid content is also typical of lipid-protein microdomains of other membranes), while only few phospholipids are present in tonoplast microdomains. The presence of microdomains has been confirmed by fluorescence and confocal microscopy using filipin and Laurdan as fluorescent probes. The experiments with Laurdan have shown that tonoplast microdomains are characterized by a high order compared to characteristics of the rest of the tonoplast. Thus, the presence of detergent-resistant lipid-protein microdomains in the tonoplast has been demonstrated.

No abstract

No abstract

No abstract

The formation of luminescent colour centres in the LiF crystal under the action of femtosecond pulses of the first harmonic of a Ti : sapphire laser is experimentally studied. The experiments were carried out at low-and high-aperture focusing of radiation. The effect of both single pulses and multi-pulse trains on the crystals was studied. Channelling of laser radiation in the waveguides, induced by the filaments of the first pulses, is found. The multiphoton mechanism of interband absorption is confirmed. The optimal conditions of laser impact for designing luminescent emitters in the LiF crystal are determined. .ru; A.V. Kirpichnikov, E.V. Pestryakov, S.N. Bagayev Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, prosp. Akad. Lavrent'eva 13/3,

No abstract

No abstract

No abstract