Pectinase production from lemon peel pomace as support and carbon source in solid-state fermentation column-tray bioreactor

Ruiz, Héctor A.; Rodríguez-Jasso, Rosa M.; Rodríguez, Raúl; Contreras-Esquivel, Juan Carlos; Aguilar, Cristóbal N.

doi:10.1016/j.bej.2012.03.007

Cited by 126 publications

(59 citation statements)

References 26 publications

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“…As regards specific rate of growth () ( Table 2), their values remain within the expected range for fungal growth by SSF (between 10 and 90·10 -3 h -1 ) (Favela-Torres et al 1998;Ruiz et al 2012;Smits et al 1996).…”

Section: Discussionsupporting

confidence: 58%

“…1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Biomass during fermentations was monitored by an indirect procedure, which turned out to be simple and quick (Ruiz et al 2012). Basically, the method consists on measuring the degree of colonization of the solid substrate with mycelium in every moment.…”

Section: Enzymes Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

Díaz

Blandino

Webb

et al. 2016

Appl Microbiol Biotechnol

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A simple kinetic model, with only three fitting parameters, for several enzymes production in Petri dishes by solid state fermentation is proposed in this paper, which may be a valuable tool for simulation of this type of processes. Basically, the model is able to predict temporal fungal enzymes production by solid state fermentation on complex substrates, maximum enzyme activity expected and time at which these maximums are reached. In this work several fermentations in solid state were performed in Petri dishes, using four filamentous fungi grown on different agro-industrial residues, measuring xylanase, exopoligalacturonase, cellulose and laccase activities over time. Regression coefficients after fitting experimental data to the proposed model turned out to be quite high in all cases. In fact, these results are very interesting considering on the one hand, the simplicity of the model, and on the other hand, that enzyme activities correspond to different enzymes, produced by different fungi on different substrates.

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Section: Discussionsupporting

confidence: 58%

Section: Enzymes Assaysmentioning

confidence: 99%

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

Díaz

Blandino

Webb

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…In SSF, smaller particle size provides a larger contact area. However, reduction in particle size increases the packing density, which causes a reduction in the void space between the particles, which results in reduction in microbial growth and enzyme production (Ruiz et al, 2012). Therefore, there must be an optimum value for particle size.…”

Section: Resultsmentioning

confidence: 99%

Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste

2014

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“…The non-significant terms were kept when their quadratic or interaction effect showed a significant (p < 0.05) effect on the response variables. The Equations (1)(2)(3)(4) show the main quadratic and interaction effects of the factors affecting the response variables. The estimated regression coefficient of independent variables, as well as R 2 and lack of fit are shown in Table 1.…”

Section: Fitting the Final Reduced Modelsmentioning

confidence: 99%

“…Due to the extensive applications of the enzyme in various industries there is a need to find novel sources of the enzymes due to their current limited production. Furthermore, utilization of agro-industrial residues for enzyme production minimizes pollution and allows the production of high value-added products using an economical process [4]. Pitaya or dragon fruit (Hylocereus polyrhizus) is among the important commercial tropical fruits in the World [5,6].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Extraction of Novel Pectinase Enzyme Discovered in Red Pitaya (Hylocereus polyrhizus) Peel

Zohdi

Amid

2013

Molecules

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Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X 1 ), extraction temperature (−15 to +25 °C, X 2 ) and buffer pH (4.0 to 12.0, X 3 ) on specific activity, temperature stability, storage stability and surfactant agent stability of pectinase from pitaya peel was investigated. The study demonstrated that the optimum conditions for the extraction of pectinase from pitaya sources could improve the enzymatic characteristics of the enzyme and protect its activity and stability during the extraction procedure. The optimum extraction conditions cause the pectinase to achieve high specific activity (15.31 U/mg), temperature stability (78%), storage stability (88%) and surfactant agent stability (83%). The most desirable conditions to achieve the highest activity and stability of pectinase enzyme from pitaya peel were the use of 5:1 buffer to sample ratio at 5 °C and pH 8.0.

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Pectinase production from lemon peel pomace as support and carbon source in solid-state fermentation column-tray bioreactor

Cited by 126 publications

References 26 publications

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste

Optimization of Extraction of Novel Pectinase Enzyme Discovered in Red Pitaya (Hylocereus polyrhizus) Peel

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