Pectin lyase production by Penicillium griseoroseum: Effect of tea extract, caffeine, yeast extract, and pectin

Abstract

Mycelial growth and production of extracellular pectin lyase by Penicillium griseoroseum at different concentrations of inducers were investigated. The fungus was cultured in mineral medium using sucrose as a carbon source and caffeine, yeast extract, tea extract or pectin as inducers. Caffeine, yeast extract and tea extract in the presence of sucrose, and tea extract alone were capable of inducing pectin lyase in P. griseoroseum, even at low concentrations.

Improved pectinase production in Penicillium griseoroseum recombinant strains

“…1993; Baracat‐Pereira et al. 1994; Minussi et al. 1996; Fawole and Odunfa 2003; Patil and Dayanand 2006).…”

“…Alternative carbon sources, such as sugar cane juice, have been assayed with the object of reducing the production costs of microbial PGs and PLs. The approach of pectinase production, using carbon sources of low cost and other substances to replace the natural inducer, pectin, has been used by several researchers (Brumano et al 1993;Baracat-Pereira et al 1994;Minussi et al 1996;Fawole and Odunfa 2003;Patil and Dayanand 2006). However, these authors had little success, because the genes encoding pectinases in filamentous fungi undergo catabolite repression by simple sugars such as glucose and sucrose (Kusters-Van Someren et al 1991;Bazzolli et al 2006;Trigui-Lahiani and Gargouri 2007).…”

Differential expression ofplggenes fromPenicillium griseoroseum:plg1 a pectinolytic gene is expressed in sucrose and yeast extract

“…Penicillium griseoroseum has long been studied aiming to find alternative inducers for the industrial production of pectic enzymes (Baracat‐Pereira et al 1994; Minussi et al 1996, 1998; Piccoli‐Valle et al . 2001a,b).…”