Pectin lyase: A review

Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755

“…These results are in agreement with our previous observation that EDTA inhibits the activity of pectin lyase (Table 2) produced by F.oxysporum. Retting of Crotolaria juncea fiber in the absence of EDTA has earlier been reported for fungal pectin lyases, namely Aspergillus flavus MTCC 7589, Aspergillus terricola MTCC 7588, Penicillium citrinum MTCC 8897, Aspergillus flavus MTCC 10938, and Fusarium decemcellulare MTCC 2079 (Yadav et al 2008(Yadav et al , 2009a(Yadav et al , b, 2013(Yadav et al , 2014. In contrast, efficient retting in the presence of EDTA has also been reported in the literature (Yadav et al 2012;DE Henrikkson et al 1997).…”

Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

“…Pectin and hemicelluloses are responsible for the integrity and coherence of plant tissues as well as for the texture of vegetables and fruits (Voragen et al 2009). These are mainly degraded by a group of enzymes referred to as pectinases, which have been classified according to their mode of action and substrate preference into pectin esterases (EC 3.1.1.11), polygalacturonases (PGs; EC 3.2.1.15), pectate lyases (EC 4.2.2.2) and pectin lyases (EC 4.2.2.10) (Yadav et al 2009). Pectinases have been extensively reviewed and have potential applications in clarification of fruit juices, retting of fibre, treatment of pectic waste water, coffee and tea leaf fermentation, oil extraction and virus purifications (Jayani et al 2005;Satyanarayana & Kumar 2005;Payasi et al 2008;Pedrolli et al 2009).…”

Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets

“…Pectin lyase and polygalacturonase are two enzymes that act on the pectin backbone. Polygalacturonase hydrolyses the α-1,4-bonds galacturonic acid residues in the pectin backbone whereas pectin lyase can cleave the pectin backbone by β-elimination (Kashyap et al 2001;Yadav et al 2009). By the action of pectin lyase and/or polygalacturonase, high-molecular weight (>100 kDa) fibers consisting of primarily galacto-rhamnogalacturonan are released both in vitro (Thomassen et al 2011, Strube et al 2015a) and in vivo in piglets (Strube et al 2015b).…”

“…The reactions were initiated by the addition of enzyme and were then followed spectrophotometrically at 235 nm (Yadav et al 2009). In a 100 µL reaction, 5 μL enzyme supernatant was used.…”