Pectin lyase is a key enzyme in the maceration of potato tuber

Abstract

:The maceration of potato tuber (Solanum tuberosum cv Bintje) by technical enzyme preparations was examined with the aid of ýuorescence microscopy using ýuorescein diacetate as a dye. Treatment with Pectinex Ultra-SP-L from Aspergillus aculeatus resulted in a higher release of single viable cells and smaller clumps of cells than treatment with Rohament P from Aspergillus niger. Fractions with high pectin lyase and low polygalacturonase activity obtained by anion exchange chromatography of Pectinex Ultra SP-L showed the highest degree of maceration. When two diþerent cloned polygalacturonases (PG1 and PG2) and a cloned pectin lyase (PL1) from A aculeatus were examined, the highest degree of maceration was observed with PL1. In conclusion, pectin lyase is probably the main enzyme in Pectinex Ultra-SP-L responsible for the maceration of potato tuber.

Fungal enzymes transferred by leaf-cutting ants in their fungus gardens

“…CMCases and pectinases could initiate hyphal colonization by attacking plant cell walls when a faecal droplet is placed on the finely fragmented leaf-material. The application of pectinases could be especially important to accelerate the early stages of degradation, due to its macerating effect on plant tissue (van Den Brock et al 1997). The ant's own metabohsm is less hkely to benefit from pectinases and CMCase, since they do not ingest entire plant-cells, but only the sap (Febvay & Kermarrec 1981).…”

Rheological Behavior and Physicochemical Characteristics of Goldenberry (Physalis Peruviana) Juice as Affected by Enzymatic Treatment

“…Application of enzyme preparations in juice production is one of the advanced technologies today. Pectinolytic enzymes are used for fruit processing to increase yields and improve liquefaction, clarification and filterability of juices (Dörreich 1996;Van den Broek et al 1997;Demir et al 2001;Ramadan and Mörsel 2007). Addition of pectinolytic enzyme to the pulps of plum, peach and apricot increased juice yield and caused a slight change in total soluble solids, pH value and acidity as well as a drastic decrease in apparent viscosity (Joshi et al 1991).…”

Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets

“…One unit of enzyme activity was defined as the amount of enzyme catalyzing the release of 1 μmol of unsaturated uronide per minute. The extinction coefficient used was 5.5 mM −1 cm −1 (van den Broek et al 1997). Reactions were done in duplicates.…”