Pectin lyase from Aspergillus sp. CH-Y-1043

Delgado, Luis Alejandro Mosquera; Trejo, Blanca A.; Huitrón, Carlos; Aguilar, Gonzalo Galicia

doi:10.1007/bf00205043

Cited by 43 publications

(26 citation statements)

References 13 publications

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“…However, this production was not significant in respect to mean production (Figure 2d). High pectin lyases production obtained at initial pH of 8.0 has also been reported for another Aspergillus lyases (Delgado et al, 1993). On the other hand, even when pectin lyase production obtained on lemon peel was considerably higher than that obtained on pectin on pH 8.0, the productivity value was almost the same (Table 2).…”

Section: Wwwrmiqorgsupporting

confidence: 75%

XYLANASES AND PECTINASES OF Aspergillus flavus CECT-2687 ON DIFFERENT CARBON SOURCES AND INITIAL pH VALUES

Pradal¹

2018

Rev. Mex. Ing. Quim.

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Aspergillus flavus is a saprophytic opportunistic plant pathogen that infects crops and stored grains, in a process assisted by the action of enzymes, such as xylanases and pectinases. The enzymatic potential of A. flavus has not been addressed in detail, even when this knowledge could be useful for the understanding of the colonization dynamics during the grains spoilage by fungi, in order to find of strategies to control it. The effect of carbon source and pH on enzyme production was evaluated by growing A. flavus CECT-2687 in liquid cultures on different monosaccharides, polysaccharides or agricultural residues, at several initial pH values. The results showed a significant effect of pH, carbon source and the interaction of both factors. Xylanases were produced on monosaccharides, although the highest xylanolytic activities were obtained on agroindustrial xylan-rich residues. Exopectinases titers were similar on polysaccharides and agricultural residues, and were not observed on monosaccharides. Pectin lyases were produced on pectin and agricultural residues; and a pectin lyase of 27 kDa was identified in crude extracts of lemon peel cultures at initial pH of 8. The highest enzymatic productions were obtained on cultures with initial pH of 8. Keywords: xylanases, exopectinases, pectin lyases, agroindustrial residues, Aspergillus flavus CECT-2687. ResumenAspergillus flavus es saprofito y patógeno oportunista de cultivos y granos almacenados, en un proceso asistido por la acción de enzimas como xilanasas y pectinasas. El potencial enzimático de A. flavus no se ha abordado en detalle, incluso cuando este conocimiento podría ser útil para comprender la dinámica de colonización de granos por hongos y encontrar estrategias para su control. El efecto de la fuente de carbono y el pH sobre la producción de enzimas se evaluó cultivando A. flavus CECT-2687 en cultivos líquidos en diferentes monosacáridos, polisacáridos o residuos agrícolas, a diferentes valores de pH. Se encontró un efecto significativo del pH, la fuente de carbono y su interacción. Las xilanasas se produjeron en monosacáridos, las actividades más elevadas se obtuvieron en residuos agroindustriales ricos en xilano. Los títulos de exopectinasas fueron similares en polisacáridos y residuos agrícolas y no se observaron en monosacáridos. Las pectin liasas se produjeron en pectina y residuos agrícolas; y se identificó una pectina liasa de 27 kDa en extractos crudos de cultivos de cáscara de limón a pH inicial de 8. La mayor producción de todas las enzimas se observó en los cultivos con pH inicial de 8.

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Section: Wwwrmiqorgsupporting

confidence: 75%

XYLANASES AND PECTINASES OF Aspergillus flavus CECT-2687 ON DIFFERENT CARBON SOURCES AND INITIAL pH VALUES

Pradal¹

2018

Rev. Mex. Ing. Quim.

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“…10) hydrolyze α-1, 4-glycosidic bond by process called transelimination releasing galacturonide with a double bond between C-4 and C-5 at the nonreducing end of the sugar backbone. Generally, polygalacturonases also attack α-1, 4-glycosidic linkages in homogalacturonans (Delgado et al, 1993). Now-a-days, they are the high flying industrial enzymes of textile industries, plant fiber processing, degumming, tea, coffee, vegetables oil extraction industries and treatment of industrial wastewater having pectinacious wastes.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Sethi¹,

Panda²,

Sahoo³

2014

Asian J. of Biotechnology

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In this study, Aspergillus terreus NCFT4269.10 was selected for the biosynthesis of pectinase after screening from fifteen native fungal strains as the performance of A. terreus was best in pectin hydrolyzing activity (2.75 cm). Aiming at the cost effective fermentation, several agro-industrial residues such as, Pearl Millet (PM), Finger Millet (FM), Orange Peels (OP), Mustard oil Cake (MoC) and Chikling Vetch Peels (CVP) were evaluated for highest pectinase production using Liquid Static Surface Fermentation (LSSF) and Liquid Shaking Fermentation (LShF). Among these substrates, orange peel was found to be the most suitable substrate for optimal pectinase biosynthesis (LSSF: 633.33±57.73 U mLG ) when the culture was grown under static and shaking condition at 30°C for 96 h. Maximum biomass was formed when OP was implemented for fermentation at static condition followed by CVP. But maximum biomass did not always support the enhanced pectinase biosynthesis in most of the cases. Partial purification of pectinase revealed a 3.86 fold purification and 27.63% yield of protein. Fermentation kinetics study revealed that purified enzyme activity yield per gram of substrate was maximum at both LSSF (1866.6 U gdsG 1 ) and LShF (2026.7 U gdsG 1 ) when biosynthesis of pectinase was carried out using OP. Ratio of enzyme yield and biomass were best with OP as compared to the other substrates both in static and shaking fermentation at crude and purified conditions.

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“…Filamentous fungi like Aspergillus niger and Hypocrea jecorina (Trichoderma reesei) have been shown to produce a wide spectrum of polysaccharide-hydrolytic enzymes. Of these fungi, A. niger is industrially used as a producer of many pectinases (Bussink et al 1992;Delgado et al 1992;Parenicová et al 2000) and hemicellulosedegrading enzymes, like xylanases and arabinases (Gielkens et al 1997;van Peij et al 1997), while H. jecorina is the most important organism used in cellulase production (Fägerstam and Pettersson 1980;Irwin et al 1993;Nidetzky et al 1994;Wood and McCrae 1979).…”

Section: Introductionmentioning

confidence: 99%

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

Stricker

Mach

Graaff

2008

Appl Microbiol Biotechnol

247

182

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Pectin lyase from Aspergillus sp. CH-Y-1043

Cited by 43 publications

References 13 publications

XYLANASES AND PECTINASES OF Aspergillus flavus CECT-2687 ON DIFFERENT CARBON SOURCES AND INITIAL pH VALUES

XYLANASES AND PECTINASES OF Aspergillus flavus CECT-2687 ON DIFFERENT CARBON SOURCES AND INITIAL pH VALUES

Biosynthesis of Industrially Relevant Extracellular Pectinase from Aspergillus terreus NCFT 4269.10 Using Orange Peels as Substrate

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

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