Peach (Prunus persica L.)

Sabbadini, Silvia; Pandolfini, Tiziana; Girolomini, Luca; Molesini, Barbara; Navacchi, O.

doi:10.1007/978-1-4939-1658-0_17

Cited by 17 publications

(12 citation statements)

References 12 publications

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“…Cultivar-independent protocols are still necessary and, so far, there have been no transgenic peaches with modified traits. Rootstock genetic engineering was successful and effective to control scion traits [201]. Peach gene function is currently addressed by transient RNA-interference technologies [202,203] and new approaches exploit development genes to enhance in vitro regeneration [204].…”

Section: Phase Change In Fruit Trees: Advances and Perspectives Inmentioning

confidence: 99%

Plant Cellular and Molecular Biotechnology: Following Mariotti’s Steps

Paolis

Frugis

Giannino

et al. 2019

Plants

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This review is dedicated to the memory of Prof. Domenico Mariotti, who significantly contributed to establishing the Italian research community in Agricultural Genetics and carried out the first experiments of Agrobacterium-mediated plant genetic transformation and regeneration in Italy during the 1980s. Following his scientific interests as guiding principles, this review summarizes the recent advances obtained in plant biotechnology and fundamental research aiming to: (i) Exploit in vitro plant cell and tissue cultures to induce genetic variability and to produce useful metabolites; (ii) gain new insights into the biochemical function of Agrobacterium rhizogenes rol genes and their application to metabolite production, fruit tree transformation, and reverse genetics; (iii) improve genetic transformation in legume species, most of them recalcitrant to regeneration; (iv) untangle the potential of KNOTTED1-like homeobox (KNOX) transcription factors in plant morphogenesis as key regulators of hormonal homeostasis; and (v) elucidate the molecular mechanisms of the transition from juvenility to the adult phase in Prunus tree species.

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Section: Phase Change In Fruit Trees: Advances and Perspectives Inmentioning

confidence: 99%

Plant Cellular and Molecular Biotechnology: Following Mariotti’s Steps

Paolis

Frugis

Giannino

et al. 2019

Plants

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“…This in vitro regeneration method, via organogenesis, has been confirmed, also by other studies, to be really effective for the regeneration and genetic transformation, not only of grapevine, but also of other plant species, as Prunus spp. and blueberry [30][31][32][33].…”

Section: Induction Of Mbs From Different Grapevine Cultivars and Rootmentioning

confidence: 99%

A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars

et al. 2019

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Worldwide grapevine cultivation is based on the use of elite cultivars, in many cases strictly linked to local important wine brands. Most of Vitis viniferacultivars have high susceptibility to fungal and viral diseases therefore, new breeding techniques (e.g. Cisgenesis, RNAi and gene editing) offer the possibility to introduce new clones of the main cultivars with increased diseases resistance, in order to reduce environmental impact and improve quality in the intensive wine grape industry. This study is finalized to develop efficient in vitro regeneration and transformation protocols to extend the application of these technologies in wine grape cultivars and rootstocks. With this aim, in vitro regeneration protocols based on the production of meristematic bulks (Mezzetti et al., 2002) were optimized for different grapevine cultivars (Glera, Vermentino, Sangiovese, Thompson Seedless) and rootstocks (1103 Paulsen, and 110 Richter). The meristematic bulks were then used as explants for Agrobacteriummediated genetic transformation protocols, by comparing the use of NPTII and e-GFP as marker genes. Results confirmed the efficiency of meristematic bulks as the regenerating tissue to produce new modified plants in almost all the above genotypes. The highest regeneration efficiency in some genotypes allowed the selection of stable modified lines/calli with only the use of e-GFP marker gene. This protocol can be applied in the use of MYB marker gene for the production of cisgenic lines. Genotypes having the highest regeneration and transformation efficiency were also used for transformation experiments using a hairpin gene construct designed to silence the RNA-dependent RNA polymerase (RpRd) of the GFLV and GLRaV3, which would induce multiple virus resistances, and the Dicer-like protein 1 (Bc-DCL1) and Bc-DCL2 to control B. cinerea infection.

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“…Although the use of adult tissues is highly recommended to preserve desirable traits of peach selected clones [ 3 ], adventitious shoots regeneration starting from seed-derived material has been the main successful approach used in the past for this species [ 4 , 5 , 6 , 7 , 8 ]. However, peach adventitious shoot regeneration, avoiding the use of juvenile tissues, has also been recorded starting from meristematic tissues [ 2 , 3 , 9 ] and leaves [ 10 , 11 , 12 , 13 ]. In particular, Gentile et al (2002) described the only study reporting in vitro shoot organogenesis from leaves of Prunus persica L. cultivars (from expanded leaves, from leaves of preconditioned shoot apices and from leaves of adventitious shoots) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Ricci

Capriotti

Mezzetti

et al. 2020

Plants

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In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 μM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.

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Peach (Prunus persica L.)

Cited by 17 publications

References 12 publications

Plant Cellular and Molecular Biotechnology: Following Mariotti’s Steps

Plant Cellular and Molecular Biotechnology: Following Mariotti’s Steps

A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars

Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

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