PDL1 And LDHA act as ceRNAs in triple negative breast cancer by regulating miR-34a

Huang, Xiaojia; Xie, Xiaoming; Wang, Hua; Xiao, Xia; Yang, Lu; Tian, Zhi; Guo, Xin; Zhang, Lijuan; Tang, Hailin; Xie, Xiaoming

doi:10.1186/s13046-017-0593-2

Cited by 47 publications

(36 citation statements)

References 38 publications

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“…Accumulating evidence has demonstrated that aberrant expression of miR-34a regulates the progression of various neoplasms (25), including glioma (26), breast cancer (27,28), cervical cancer (29), cholangiocarcinoma (30) and multiple myeloma (31). The results of the present study demonstrated that the expression level of miR-34a was reduced in RB tissues compared with that in paracarcinoma tissues (P<0.05).…”

Section: Discussionsupporting

confidence: 50%

miR‑34a regulates the chemosensitivity of retinoblastoma cells via modulation of MAGE‑A/p53 signaling

Yang

et al. 2018

Int J Oncol

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The present study aimed to explore the combined role of microRNA (miR)-34a, melanoma antigen-A (MAGE-A) and p53 in altering the chemosensitivity of retinoblastoma (RB) cells. Human RB and adjacent tumor tissues, as well as human RB cell lines (HXO-Rb44, SO-Rb50, Y79 and WERI-Rb-1) were used. In addition, four chemotherapeutic drugs, including carboplatin, etoposide, Adriamycin and vincristine, were used to treat the cell lines, in order to evaluate the sensitivity of RB cells. Furthermore, miR-34a expression was detected by reverse transcription-quantitative polymerase chain reaction, and western blotting was implemented to quantify expression levels of MAGE-A and p53. A luciferase reporter gene assay was used to validate the targeted association between miR-34a and MAGE-A. The results indicated that SO-Rb50 cells exhibited the highest resistance to carboplatin, Adriamycin and vincristine (P<0.05), whereas HXO-Rb44 cells revealed the highest inhibition rate in response to etoposide (P<0.05) out of the four cell lines. Furthermore, reduced miR-34a expression and increased MAGE-A expression significantly elevated the survival rate and viability of SO-Rb50 cells following drug treatment (all P<0.05). miR-34a was also demonstrated to directly target MAGE-A, thereby significantly promoting the viability of RB cells and depressing apoptosis (P<0.05). p53, which was subjected to modulation by miR-34a and MAGE-A, also significantly reduced the proliferation rate of RB cells (P<0.05). In conclusion, the miR-34a/MAGE-A/p53 axis may be conducive to enhancing the efficacies of chemotherapeutic treatments for RB.

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Section: Discussionsupporting

confidence: 50%

miR‑34a regulates the chemosensitivity of retinoblastoma cells via modulation of MAGE‑A/p53 signaling

Yang

et al. 2018

Int J Oncol

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“…20 with poor survival, such as breast cancer, 28 colorectal cancer, 29 pancreatic cancer. 20 with poor survival, such as breast cancer, 28 colorectal cancer, 29 pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

“…LDHA, as one of the key metabolic enzyme, is essential for the conversion of pyruvate into lactate, and might be involved in tumor initiation and metabolism by regulating warburg effect. Increased LDHA has been observed in variety of tumors and is correlated with poor survival, such as breast cancer, 28 colorectal cancer, 29 pancreatic cancer. 30 Additionally, several studies have found that LDHA silencing could reduce tumor occurrence and growth, suppress metastasis and increase apoptosis in many cancers.…”

Section: Discussionmentioning

confidence: 99%

LDHA is a direct target of miR‐30d‐5p and contributes to aggressive progression of gallbladder carcinoma

Chen

et al. 2018

Molecular Carcinogenesis

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Gallbladder cancer (GBC) is the most general biliary tract malignancy, with poor prognosis due to rapid tumor progression and lack of specific symptoms. Lactate dehydrogenase-A (LDHA) can promote Warburg effect to produce lactate and Adenosine Triphosphate (ATP) in aerobic condition, which contributes to oncogenesis metastasis and drug resistance in various cancers. However, the expression and functional role of LDHA in GBC are largely unknown. We determined that LDHA was over-expressed in GBC tumor tissues compared with normal tissues, which was also an independent prognostic factor for the overall survival of GBC patients by tissue microarrays analysis. In addition, RNAi-mediated LDHA silencing could suppress the GBC cell proliferation, invasion, colony formation and glycolysis while promoting cell apoptosis in vitro. Similar results were observed in GBC cells treated with LDHA specific inhibitor FX11. Moreover, we confirmed that knockdown of LDHA could inhibit tumor growth in vivo. Additionally, we found that the 3'-untranslated region (3'-UTR) of LDHA mRNA was the direct target of microRNA-30d-5p (miR-30d-5p), which was low expressed in GBC tissues and associated with poor prognosis of GBC patients. Our findings disclose a novel role for miR-30d-5p/LDHA axis contribute to aggressive progression by reprogramming the metabolic process in GBC cells, and suggest a potential application of miR-30d-5p/LDHA axis in prognosis prediction and GBC treatment.

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“…The term microRNA (miRNA) signatures has attracted increased attention in recent years, which could potentially be used as predictive or prognostic biomarkers . The combined analysis of a panel of biomarkers, rather than individual analyses, as a nomogram is the most promising approach that is powerful enough to change clinical management .…”

mentioning

confidence: 99%

“…The term microRNA (miRNA) signatures has attracted increased attention in recent years, which could potentially be used as predictive or prognostic biomarkers. [8][9][10][11][12][13] The combined analysis of a panel of biomarkers, rather than individual analyses, as a nomogram is the most promising approach that is powerful enough to change clinical management. 7,[14][15][16] Although classifier models based on clinical-associated factors, such as axillary ultrasound (AUS) and tumor size, have been investigated and demonstrated to be useful for ALN metastasis prediction in patients with BC, an optimal approach that combines multiple miRNAs biomarkers as a predictive signature has yet to be developed.…”

mentioning

confidence: 99%

Preoperative prediction nomogram based on primary tumor miRNAs signature and clinical‐related features for axillary lymph node metastasis in early‐stage invasive breast cancer

Xie

Tan

Chen

et al. 2018

Intl Journal of Cancer

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More than half patients who undergo axillary lymph node (ALN) surgery are ALN negative in early-stage invasive breast cancer (EIBC). Thus, to avoid excessive treatment, we aim to establish and validate a novel nomogram model for the preoperative diagnosis of ALN status in patients with EIBC. In total, 864 patients with EIBC from two independent centers were enrolled in our study. For the discovery set, miRNAs expression profiling with functional roles in ALN metastasis was discovered by microarray analysis and validated by quantitative polymerase chain reaction (PCR). For the training and validation cohorts, we used PCR to quantify miRNAs expression in a model development cohort and assessed miRNAs signature in an internal validation cohort and external independent validation cohort. Multivariable logistic regression analyses were used to establish a nomogram model for the likelihood of ALN metastasis from miRNAs signature and clinical variables. A signature of nine-miRNA was significantly associated with ALN status. The predictive ability of our nomogram that included miRNAs signature and clinical-related variables (age, tumor size, tumor location and axillary ultrasound-reported ALN status) was significantly greater than a model that only considered clinical-related factors (concordance index: 0.856, 0.796) and also performed well in the two validation cohorts (concordance index: 0.841, 0.747). Our nomogram is a reliable prediction method that can be conveniently used to preoperatively predict ALN status in patients with EIBC. Therefore, after further confirmation in prospective and multicenter clinical trial, omission of axillary surgery may be feasible for some patients with EIBC in the future.

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PDL1 And LDHA act as ceRNAs in triple negative breast cancer by regulating miR-34a

Cited by 47 publications

References 38 publications

miR‑34a regulates the chemosensitivity of retinoblastoma cells via modulation of MAGE‑A/p53 signaling

miR‑34a regulates the chemosensitivity of retinoblastoma cells via modulation of MAGE‑A/p53 signaling

LDHA is a direct target of miR‐30d‐5p and contributes to aggressive progression of gallbladder carcinoma

Preoperative prediction nomogram based on primary tumor miRNAs signature and clinical‐related features for axillary lymph node metastasis in early‐stage invasive breast cancer

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