LDHA is a direct target of miR‐30d‐5p and contributes to aggressive progression of gallbladder carcinoma

He, Yuting; Chen, Xiaolong; Yu, Yan; Li, Juan; Hu, Qiuyue; Xue, Chen; Chen, Jianan; Shen, Shen; Luo, Yi; Ren, Fang; Li, Chao; Bao, Jie; Yan, Jingya; Qian, Guowu; Ren, Zhigang; Sun, Ranran; Cui, Guangying

doi:10.1002/mc.22799

Cited by 62 publications

(44 citation statements)

References 35 publications

(64 reference statements)

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“…Western blot analysis was performed as previously reported . Briefly, total cell protein lysates were separated by 10% SDS‐PAGE and transferred to a nitrocellulose membrane (Beyotime, China).…”

Section: Methodsmentioning

confidence: 99%

“…Western blot analysis was performed as previously reported. 22 Briefly, total cell protein lysates were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (Beyotime, China). After incubation with specific antibodies and 5% skim milk powder, the NC membranes were then incubated with specific primary antibodies, followed by HRP-labeled secondary antibody (Beyotime, China) and detected by chemiluminescence.…”

Section: Western Blotting Assaymentioning

confidence: 99%

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CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

Han

Cao

et al. 2019

Cancer Medicine

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Osteosarcoma is the most common type of primary malignant tumor of skeletal with poor prognosis in children and adolescents. Accumulating evidence indicates that CBX2 is overexpressed in multiple human neoplasm and play a critical role in tumorigenesis and progression. However, its functional role and upstream regulation mechanism in osteosarcoma remain unknown. In the present study, tissue microarray (TMA) analysis was performed to determine the association between CBX2 expression and clinical prognosis of osteosarcoma patients by immunohistochemistry. We also investigated the functional role of CBX2 using small interfering RNA (siRNA) in vitro and in vivo. Additionally, we confirmed the direct binding between CBX2 and let‐7a via qPCR, western blot and luciferase reporter assay. We found that CBX2 is dramatically upregulated in osteosarcoma tissues and high CBX2 expression was correlated with metastasis, recurrence, and chemotherapy response, as well as unfavorable prognosis in patients with osteosarcoma. Similar results were observed in a sarcoma cohort from The Cancer Genome Atlas (TCGA) dataset. Further experiments revealed that CBX2 knockdown significantly impeded osteosarcoma cell proliferation and invasion ability in vitro, and suppressed the tumor growth in tumor xenografts model. Mechanistically, we confirmed that CBX2 is a functional target of miRNA let‐7a. Overexpression of let‐7a inhibits osteosarcoma cell proliferation, which was reversed by CBX2 overexpression. Taken together, our study demonstrates that let‐7a/CBX2 plays a crucial role in osteosarcoma progression. CBX2 could serve as a promising prognostic biomarker and potential therapeutic target for osteosarcoma patients.

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Section: Methodsmentioning

confidence: 99%

Section: Western Blotting Assaymentioning

confidence: 99%

CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

Han

Cao

et al. 2019

Cancer Medicine

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“…MicroRNA (miRNA), a kind of small non‐coding RNA, could inhibit translation or promote degradation of targets via combining to the specific region of 3′‐untranslated region (3′‐UTR) in targeted mRNA . Until now, several miRNAs like miR‐34a, miR‐34c, miR‐369‐3p, miR‐374a, miR‐30a‐5p, miR‐142‐3p, miR‐30d‐5p, miR‐323a‐3p, miR‑199a‑3p, miR‐449a, and miR‐4524a/b have been found to target the mRNA of LDHA . In a recent study on colorectal cancer, miR‐34a, miR‐34c, miR‐369‐3p, miR‐374a, and miR‐4524a/b were established to directly inhibit LDHA .…”

Section: Regulation Modes Of Ldhamentioning

confidence: 99%

Lactate dehydrogenase A: A key player in carcinogenesis and potential target in cancer therapy

et al. 2018

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Elevated glycolysis remains a universal and primary character of cancer metabolism, which deeply depends on dysregulated metabolic enzymes. Lactate dehydrogenase A (LDHA) facilitates glycolytic process by converting pyruvate to lactate. Numerous researches demonstrate LDHA has an aberrantly high expression in multiple cancers, which is associated with malignant progression. In this review, we summarized LDHA function in cancer research. First, we gave an introduction of structure, location, and basic function of LDHA. Following, we discussed the transcription and activation mode of LDHA. Further, we focused on the function of LDHA in cancer bio‐characteristics. Later, we discussed the clinical practice of LDHA in cancer prevention and treatment. What we discussed gives a precise insight into LDHA especially in cancer research, which will contribute to exploring cancer pathogenesis and its handling measures.

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“…Western blot analysis was performed as previously described [21]. Quantification analysis of western blot was conducted with the software ImageJ (Bethesda, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition

Cao¹,

Han²,

Li³

et al. 2020

J. Cancer

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Background: Cervical cancer is one of the most common gynaecological malignancies. Emerging studies have documented that prolyl-4-hydroxylase α subunit 2 (P4HA2) is involved in multiple processes of cancer progression. However, the functional roles of P4HA2 in cervical cancer progression remain to be elucidated. Methods: P4HA2 mRNA and protein levels were examined in cervical cancer tissues and cell line by qRT-PCR and western blot. The correlation of the P4HA2 expression levels and prognosis of cervical cancer patients were analysed in TCGA cervical cancer cohort and tissue microarray (TMA) cohort. P4HA2 was silenced to evaluate its function on cervical cancer progression both in vitro and in vivo. Bioinformatics analysis was performed to investigate the underlying regulation mechanism of cervical cancer by P4HA2. Results: We found that P4HA2 are markedly upregulated in cervical cancer tissues in comparison with adjacent non-neoplastic tissues. In addition, upregulation of P4HA2 was associated with shorter overall survival (OS) and relapse-free survival (RFS). Functionally, we demonstrated that P4HA2 knockdown attenuated cell proliferation, migration and invasion of cervical cancer cells. Furthermore, xenograft tumor mouse model experiment showed silencing P4HA2 significantly inhibited tumor growth in vivo. Mechanistically, bioinformatics analysis revealed that epithelial-mesenchymal transition (EMT) was involved in cervical cancer progression regulated by P4HA2 and we further confirmed knockdown P4HA2 suppressed the EMT process. Conclusion: our results suggest that P4HA2 functions as an oncogene in promoting cervical cancer cell proliferation, migration and invasion by inducing EMT, which might be a promising prognostic factor and therapeutic target for cervical cancer.

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LDHA is a direct target of miR‐30d‐5p and contributes to aggressive progression of gallbladder carcinoma

Cited by 62 publications

References 35 publications

CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

Lactate dehydrogenase A: A key player in carcinogenesis and potential target in cancer therapy

P4HA2 contributes to cervical cancer progression via inducing epithelial-mesenchymal transition

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