PDGF suppresses the activation of group II phospholipase A₂gene expression by interleukin I and forskolin in mesangial cells

Cytokine-stimulated secretion of group II phospholipase A2 by rat mesangial cells. Its contribution to arachidonic acid release and prostaglandin synthesis by cultured rat glomerular cells.

“…The cytokine effect is blocked by actinomycin D and cycloheximide, thus demonstrating a requirement for both transcription and de novo protein synthesis. This is confirmed by the observation that IL-1 and TNFa increase the level of mRNA for group II PLA2 in rabbit articular chondrocytes (24,25), rat vascular smooth muscle cells (26), rat astrocytes (27), rat mesangial cells (28,29), and human hepatoma cells (30).…”

“…Renal glomeruli from male SpragueDawley rats or from female Wistar rats (80-100 g body wt) were isolated under sterile conditions by a sieving technique, and glomerular cells were cultured as described previously (28,42). In a second step, single cells were cloned by limited dilution in 96-microwell plates to obtain pure cultures of epithelial cells and mesangial cells (28,43 tissue were minced, passed through a sterile 240 Jim stainless steel sieve, and suspended in HBSS. This suspension was then poured through a 180 jim stainless sieve followed by a 100 gm mesh.…”

Synergistic effects of PDGF-BB and cAMP-elevating agents on expression of connexin43 in mesangial cells

“…Counteracting effects between PDGF-BB and cAMP-elevating agents in the control of cell growth, migration, and other cell behaviors have been extensively documented (18,21,26), whereas little is known about the synergism between PDGF-BB and cAMP.…”

Modulatory role of platelet-derived growth factor on cytokine-induced nerve growth factor synthesis in rat glomerular mesangial cells

“…Findings that PDGF receptor expression is up-regulated in kidney rejection and that PDGF production is enhanced in active glomerulonephritis [16,[32][33][34][35] imply an important role for PDGF in immune responses in the kidney. Furthermore, antibodies to PDGF inhibit mesangial cell proliferation and matrix expansion seen in rats with mesangial proliferative nephritis induced by an antibody to the Thy-I antigen that is expressed on mesangial cells [36].…”