PCR versus culture in the detection of vaginal<i>Ureaplasma urealyticum</i>and<i>Mycoplasma hominis</i>

Petrikkos, George; Hadjisoteriou, M.; Daikos, George L.

doi:10.1016/j.ijgo.2006.12.014

Cited by 17 publications

(13 citation statements)

References 6 publications

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“…Over recent years, PCR has gained in popularity as a method for finding all genital mycoplasmas in research settings and seems to be more reliable. For example, Petrikkos and colleagues [4] examined vaginal specimens collected from 203 asymptomatic women in Greece. When culture was used as the reference standard, they found that the sensitivity and specificity of PCR for the detection of U. urealyticum were 94.2% and 92% and for M. hominis were 95.6% and 86.9%.…”

Section: Detection Of Genital Mycoplasmasmentioning

confidence: 99%

Role of Mycoplasma and Ureaplasma Species in Female Lower Genital Tract Infections

Patel

Nyirjesy

2010

Curr Infect Dis Rep

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Genital mycoplasmas are commonly found in the female genital tract. Despite ongoing debate, the evidence that they cause lower genital tract disease in women remains sparse. The data that Mycoplasma genitalium is primarily transmitted sexually are accumulating, but its role as a cause of symptomatic urethritis or cervicitis is open to debate. Although Mycoplasma hominis may be a co-factor in bacterial vaginosis, it has otherwise not been implicated as a cause of lower tract disease. Now that Ureaplasma urealyticum has been divided into U. urealyticum and Ureaplasma parvum, their role in causing urethritis and cervicitis remains even more unclear. To date, no convincing evidence exists that antimicrobial therapy should be directed solely at these organisms when treating women with urethritis, bacterial vaginosis, trichomoniasis, or cervicitis.

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Section: Detection Of Genital Mycoplasmasmentioning

confidence: 99%

Role of Mycoplasma and Ureaplasma Species in Female Lower Genital Tract Infections

Patel

Nyirjesy

2010

Curr Infect Dis Rep

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“…13,14 There are some data that NAATs may be more sensitive than standard culture (sensitivity = 92.4%, specificity = 94.8% in one study of vaginal specimens). 15 Given the significant incidence of Ureaplasma as etiology for NGU and the significance of extragenital STIs in MSM, further research on the value of screening for urethral and rectal Ureaplasma infections in YMSM is merited, especially for those engaging in condomless anal intercourse.…”

Section: Discussionmentioning

confidence: 99%

A Case of Rectal Ureaplasma Infection and Implications for Testing in Young Men Who Have Sex with Men: The P18 Cohort Study

et al. 2017

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Ureaplasma is a significant cause of nongonococcal urethritis. This is a case of rectal Ureaplasma found on culture in a young man who has sex with men not previously reported in the literature. Nucleic acid amplification tests are now standard of care for sexually transmitted infection testing, but they do not test for Ureaplasma and, therefore, may be missing important infections. Ureaplasma could have important implications in urethritis and rectal HIV transmission among men who have sex with men engaging in condomless anal intercourse. Further study of Ureaplasma's role as a rectal pathogen may be warranted.

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“…Since then PCR methods have been increasingly used in the diagnosis of Ureaplasma infections and in the study of this pathogen. PCR methods have advantages over traditional culture methods: organism identification can occur even if the numbers of bacteria are low, viability is not necessary, and rapid identification within 24 h is possible [29]. Subtyping of isolates can be performed faster with PCR methods (24-48 h after sample collection) than with culture methods [13].…”

Section: Diagnosticmentioning

confidence: 99%

Ureaplasmas

Juhász

Ostorházi

Pónyai

et al. 2011

Reviews in Medical Microbiology

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The two existing human pathogen Ureaplasma species containing 14 serovars are associated with a variety of maladies. Colonizing the human urogenital tract, ureaplasmas cause infections mainly in these organs. However, in the special population of immunosuppressed patients or preterm and very low birth weight newborns such serious Ureaplasma infections such as meningitis or pericarditis have been described. Because they lack a cell wall, ureaplasmas are very susceptible to drying and other adverse environmental conditions; therefore, careful attention has to be given to specimen collection and transportation. Commercially available culture based tests made the laboratory diagnosis of Ureaplasma species much easier. PCR methods provide further facilities to get faster diagnosis, simultaneously with serovar identification, quantitation of Ureaplasma or detection of other sexually transmitted infectious pathogens. Although fluoroquinolone, macrolide, and tetracycline resistant strains are known, antibiotic treatment of Ureaplasma infections is not problematic for now. The mechanism of antibiotic resistance has not been entirely understood till now. Biofilmforming ability of Ureaplasmas can be important in chronic infections and antibiotic resistance. The basic microbiology of Ureaplasma species, such as clinical manifestations, the diagnostic opportunities, and the therapeutic options are reviewed in the current article.

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PCR versus culture in the detection of vaginalUreaplasma urealyticumandMycoplasma hominis

Cited by 17 publications

References 6 publications

Role of Mycoplasma and Ureaplasma Species in Female Lower Genital Tract Infections

Role of Mycoplasma and Ureaplasma Species in Female Lower Genital Tract Infections

A Case of Rectal Ureaplasma Infection and Implications for Testing in Young Men Who Have Sex with Men: The P18 Cohort Study

Ureaplasmas

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