PCR screening of antimicrobial resistance genes in faecal samples from Australian and Chinese children

Ravensdale, Joshua T.; Xian, Darren Ten Wei; Wei, Chooi Ming; Lv, Quanjun; Wen, Xiajie; Guo, Jing; Coorey, Ranil; Lesouëf, Peter; Lu, Fengmin; Zhang, Guicheng; Dykes, Gary A.

doi:10.1016/j.jgar.2018.03.003

Cited by 2 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While geographical variation had been demonstrated in resistance rates of E. coli isolates in feces of healthy children ( Sahoo et al., 2012 ), resistances to commonly used antibiotics like ampicillin, tetracycline and trimethoprim/sulfamethoxazole were frequently observed, especially in developing countries ( Bartoloni et al., 2006 ; Dyar et al., 2012 ; Shakya et al., 2013 ; Ferjani et al., 2018 ; Mahmoodi et al., 2020 ). Over 90% of faecal samples from Chinese children tested positive for β-lactam, macrolide, tetracycline, and aminoglycoside resistance genes ( Ravensdale et al., 2018 ), indicating the magnitude of the problem of antimicrobial drug resistance in China. To date, however, only a handful of reports have described antibiotic resistance in faecal E. coli among Chinese healthy children ( Lester et al., 1990 ; Huang et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Fluoroquinolone-Resistant Escherichia coli in Healthy Children

Zhao

Chen

Luo

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Faecal E. coli can act as reservoirs for resistance genes. Here, we analyzed prevalence of drug resistance in faecal E. coli isolated from healthy children at a single kindergarten in Beijing, China, then used whole genome sequencing to characterize fluoroquinolone-non-susceptible strains. Our results revealed high resistance to ampicillin (54.0%), trimethoprim/sulphurmethoxazole (47.5%) and tetracycline (58.9%) among 576 faecal E. coli isolates, 49.2% of which exhibited multidrug resistance. A total of 113 E. coli isolates were not susceptible to ciprofloxacin, with four sequence types, namely ST1193 (25.7%), ST773 (13.3%), ST648 (8.8%) and ST131 (7.1%) found to be the most prevalent (54.9%). With regards to resistance to quinolones, we detected chromosomal mutations in gyrA, parC, and parE in 111 (98.2%), 105 (92.9%), and 67 (61.1%) isolates, respectively. blaCTX-M (37.2%) was the major ESBL gene, whereas blaCTX-M-14 (12.4%) and blaCTX-M-27 (11.5%) were the most frequent subtypes. A total of 90 (79.6%) ExPEC and 65 (57.5%) UPEC isolates were classified. Overall, these findings revealed clonal spread of certain prevalent STs, namely ST1193, ST773, ST648 and ST131 E. coli isolates in healthy children within a single kindergarten in Beijing, China, affirming the seriousness of the multidrug resistance problem and potential pathogenicity of E. coli isolates in healthy children. Therefore, there is an urgent need for increased surveillance to enhance control of this problem.

show abstract