Prevalence and Molecular Characterization of Fluoroquinolone-Resistant Escherichia coli in Healthy Children

Abstract

Faecal E. coli can act as reservoirs for resistance genes. Here, we analyzed prevalence of drug resistance in faecal E. coli isolated from healthy children at a single kindergarten in Beijing, China, then used whole genome sequencing to characterize fluoroquinolone-non-susceptible strains. Our results revealed high resistance to ampicillin (54.0%), trimethoprim/sulphurmethoxazole (47.5%) and tetracycline (58.9%) among 576 faecal E. coli isolates, 49.2% of which exhibited multidrug resistance. A total of 113 E. coli isolates were not susceptible to ciprofloxacin, with four sequence types, namely ST1193 (25.7%), ST773 (13.3%), ST648 (8.8%) and ST131 (7.1%) found to be the most prevalent (54.9%). With regards to resistance to quinolones, we detected chromosomal mutations in gyrA, parC, and parE in 111 (98.2%), 105 (92.9%), and 67 (61.1%) isolates, respectively. blaCTX-M (37.2%) was the major ESBL gene, whereas blaCTX-M-14 (12.4%) and blaCTX-M-27 (11.5%) were the most frequent subtypes. A total of 90 (79.6%) ExPEC and 65 (57.5%) UPEC isolates were classified. Overall, these findings revealed clonal spread of certain prevalent STs, namely ST1193, ST773, ST648 and ST131 E. coli isolates in healthy children within a single kindergarten in Beijing, China, affirming the seriousness of the multidrug resistance problem and potential pathogenicity of E. coli isolates in healthy children. Therefore, there is an urgent need for increased surveillance to enhance control of this problem.

Commensal Fitness Advantage May Contribute to the Global Dissemination of Multidrug-Resistant Lineages of Bacteria—The Case of Uropathogenic E. coli

“…Several research groups have reported appreciable/considerable commensal carriage rates for ST131-H30 strains in healthy populations [ 2 , 3 , 4 , 5 , 15 , 16 , 17 , 18 ]. Interestingly both Zhao et al [ 16 ] and Cherubini et al [ 17 ] also detected colonization with ST1193 strains. In Zhao et al’s [ 16 ] study the ST1193 strains comprised the largest group of E. coli colonizing healthy children.…”

“…Interestingly both Zhao et al [ 16 ] and Cherubini et al [ 17 ] also detected colonization with ST1193 strains. In Zhao et al’s [ 16 ] study the ST1193 strains comprised the largest group of E. coli colonizing healthy children.…”

Antibacterial activity of multidrug resistance Escherichia coli by some natural products

“…The results showed that E. coli are resistant to a number of antibiotics, including ampicillin, ampicillin/sulbactam, aztreonam, cefazolin, ceftazidime, ceftriaxone, cefepime, ciprofloxacin, gentamicin, moxifloxacin, nitrofurantoin, piperacillin/tazobactam, tobramycin, trimethoprim (Aabed et al, 2021). E. coli isolated from healthy children in China has extreme resistance to Ampicillin (54.0%), Arimethoprim/Sulphurmethoxazole (47.5%) and Tetracycline (58.9%) (Zhao et al, 2021). This research results showed that E. coli bacteria are resistant to a number of antibiotics, including ampicillin, ampicillin/sulbactam, aztreonam, cefazolin, ceftazidime, ceftriaxone, cefepime, ciprofloxacin, gentamicin, moxifloxacin, nitrofurantoin, beprazobactamide.…”

Antibiotic Resistance and Mechanisms of Pathogenic Bacteria in Tubo-Ovarian Abscess

“…Mutations in the genes for DNA gyrase ( gyrA ) and topoisomerase IV ( parC ) are the main cause of resistance to these drugs, also known as the quinolone resistance-determining regions. Mutations in the genes gyrB and parE can also weaken the therapeutic effect of (fluoro) quinolones ( Hastak et al., 2020 ; Zhao et al., 2021 ). Target site mutation is a potential strategy for combating drug resistance.…”