PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss

Zhang, W.; Sun, Zhihong; Sun, Tiansong; Zhang, H.

doi:10.1007/s12223-010-0097-3

Cited by 4 publications

(5 citation statements)

References 27 publications

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“…Fermentation occurs naturally at ambient temperature after the addition of filtrated mare milk into the container with old koumiss, which serves as the starter culture ( Zhang and Zhang, 2011 ). Koumiss is a good source of novel bacteria of biotechnology potential ( Zhang et al, 2010a ; Pan et al, 2011 ). Therefore, it is of intense interest to explore and preserve as many fermentation-associated koumiss bacteria as possible.…”

Section: Introductionmentioning

confidence: 99%

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

Yao

Hou

et al. 2017

Front. Microbiol.

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Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified. In addition to the previously reported koumiss-associated species, such as Lactobacillus (L.) helveticus. Lactococcus lactis. L. buchneri, L. kefiranofaciens, and Acetobacter pasteurianus, we successfully detected three low-abundant taxa in the samples, namely L. otakiensis. Streptococcus macedonicus, and Ruminococcus torques. The functional koumiss metagenomes carried putative genes that relate to lactose metabolism and synthesis of typical flavor compounds. Our study would encourage the use of modern metagenomics to discover novel species of bacteria that could be useful in food industries.

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Section: Introductionmentioning

confidence: 99%

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

Yao

Hou

et al. 2017

Front. Microbiol.

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“…On the other hand, several other studies have reported high proportion of Lb. helveticus in koumiss (Uchida et al, 2007;Watanabe et al, 2008;Wu et al, 2009;Miyamoto et al, 2010;Sun et al, 2010 a;Yu et al, 2011). The reason for this difference is unclear and merits further investigations.…”

Section: Discussionmentioning

confidence: 92%

“…During the last decade, numerous studies have been conducted on the bacterial communities of traditional fermented milk products from Inner Mongolia and Mongolia (formerly Outer Mongolia), using traditional cultivation in combination with a simultaneous or subsequent differentiation of the isolates based on numerously biochemical and physiological tests or by 16S rRNA gene sequencing and characterization of potential probionts (e.g., An et al, 2004;Menghe et al, 2004;Danova et al, 2005;Batdorj et al, 2006;Shuanguan et al, 2006;Uchida et al, 2007;Watanabe et al, 2008;Guo et al, 2009;Watanabe et al, 2009;Wu et al, 2009;Bao et al, 2010;Sun et al, 2010aSun et al, , 2010bZhang et al, 2010;Yu et al, 2011). However, culture-dependent methods do not reflect the true bacteriological community, but rather the needs of different growth media for cultivation of certain bacteria.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods

et al. 2014

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The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli), Acinetobacter, Bacillus and Psychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genus Lactobacillus was dominant, but Streptococcus thermophilus, Acetobacter pasteurianus and uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed: Lactococcus lactis, Lactococcus lactis subsp. lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeya sp., Enterobacter sp., Pseudomonas sp., Raoultella sp., and Ruminococcus sp.. In vitro growth inhibition of three human pathogens, Escherichia coli, Enterobacter sakazakii and Staphylococcus aureus by 14 culturable bacteria displayed that only three of the isolates tested inhibit growth of E. sakazakii while most of the other bacteria delayed growth of the target bacteria.

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“…Traditionally, it was produced from the milk of horses by residents in Central Asia and China, which is one of the most important basic food products (19). There has been an increasing interest in the production of koumiss at the industrial level due to its biotechnological potential and its benefits to human nutrition and health (20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Effect of cow colostrum, mare milk, and human milk on the viability of lung healthy and cancer cell lines

2021

ircmj

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This study investigated the effects of lyophilized mare milk, human milk, and cow colostrum on both human lung cancer cell lines, called A549, and healthy lung cell lines, called MRC5. Mare milk, human milk, and cow colostrum varieties were applied to 6 replicates in both cell lines with lyophilized milk concentrations ranging from 50-3,200 ppm. The cell viability was monitored by optic microscopy and determined by the MTT test. ANOVA and Duncan's multiple range tests were used to analyze data. The results of this study indicated that the most effective milk type on reducing the A549 lung cancer cell line was human milk, followed by mare milk; however, cow colostrum showed little effect. It was observed that human milk and mare milk had anti-proliferative effects on lung cancer cell line at concentrations which were non-toxic to healthy lung cell line.

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PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss

Cited by 4 publications

References 27 publications

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique

Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods

Effect of cow colostrum, mare milk, and human milk on the viability of lung healthy and cancer cell lines

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