PCR-RFLP detection of Haemoproteus spp. (Haemosporida: Haemoproteidae) in pigeon blood samples from Iran

Tavassoli, Mousa; Esmaeilnejad, Bijan; Malekifard, Farnaz; Mardani, Karim

doi:10.15547/bjvm.2014

Cited by 7 publications

(8 citation statements)

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“…The domestic species of Columba livia were infected by hHAECOL1 ( H. columbae ) which has been reported in same host in different geographic regions such as Botswana, Nigeria, Colombia, Italy, and Brazil ( Chagas et al, 2016 ; González et al, 2015 ; Kawo, 2012 ; Scaglione et al, 2015 ; Waldenstrom et al, 2002 ). This species has been reported as a common blood parasite in previous morphological studies conducted in Iran, using light microscopy ( Nematollahi et al, 2012 ; Samani et al, 2013 ) and molecular studies using PCR-RFLP method ( Doosti et al, 2014 ; Tavassoli et al, 2018 ). The current study is the first report of hHAECOL1 lineage using molecular methods of blood parasites and DNA sequencing in Iran.…”

Section: Discussionmentioning

confidence: 97%

“…Although several studies have been carried out on the incidence of blood parasites in wild birds in different localities using microscopic examinations in Iran ( Nourani et al, 2017a , 2018a , 2018b ; Rassouli et al, 2017 ; Shirazi et al, 2012 ), few have used molecular methods for detection of the haemosporidian parasites in this region ( Nourani et al, 2018b , Nourani et al, 2020b ). Previous morphological and molecular studies have focused on detection of haemosporidian parasites in Columbidae, especially Columba livia and passerine species ( Doosti et al, 2014 ; Fakhar et al, 2013 ; Gorji et al, 2012 ; Nourani et al, 2017a , 2018a , Nourani et al, 2020 , Nourani et al, 2020b ; Tavassoli et al, 2018 ). Recent studies have shown the presence of parasitic genera in wild passerine birds in Iran: the prevalence of 22.09% was recorded for Haemoproteus infection in north-west of Iran.…”

Section: Introductionmentioning

confidence: 99%

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Detection of haemosporidian parasites in wild and domestic birds in northern and central provinces of Iran: Introduction of new lineages and hosts

Nourani

Djadid

Rabiee³

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Haemosporidian parasites characterize multi-host and multi-parasite structures which are prevalent among wild bird populations. Here, determination of host records, estimation of the prevalence and diversity of haemosporidian lineages were performed in wild and domestic birds in 11 provinces in Iran. To our knowledge, for the first time in this region, molecular characterization of haemosporidians in migratory water birds, raptors, and domestic birds was carried out: blood or tissue samples were collected from 246 birds belonging to 36 species, 12 families, and 11 orders. The prevalence of Plasmodium , Haemoproteus, and Leucocytozoon were documented as 1.21%, 3.65%, and 0.4%, respectively. Of 36 birds' species inspected in this investigation, 13 individuals of 9 species were parasitized by blood parasites. To our knowledge, five lineages including hANACRE03, hAYTFER01, hAYTFER02, hAQUCYR01, and hSTAL06 were found as un-described lineages, while six known lineages of hLK03, pLK05, lTUSW04, pSW5, hMILANS02, and hHAECOL1 were recorded in hosts within novel geographical regions. Such results are required to fill the gaps in understanding the geographical distribution patterns of wildlife related vector-borne parasites in migratory birds as potential carriers, raptors with high vulnerability, and domestic birds as pet or with economic value.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Detection of haemosporidian parasites in wild and domestic birds in northern and central provinces of Iran: Introduction of new lineages and hosts

Nourani

Djadid

Rabiee³

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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“…In the present study, molecular detection was higher than blood smears. Previous studies have indicated comparable high sensitivity to PCR when compared to microscopy for the diagnosis of avian Haemoproteus or Malaria [ 18 , 25 , 26 ]. Further, the higher sensitivity of PCR indicates their availability to detect the infection in contrast to microscopy and to reduce possible bias in estimating the prevalence of avian blood parasites [ 7 , 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, polymerase chain reaction (PCR) has been used successfully to diagnose blood parasite infections and provides more sensitivity and accuracy than microscopic examination. Moreover, DNA sequencing helps to identify the closely related parasites and their evolutionary [ 7 , 16 – 18 ]. The sequence analysis of mitochondrial cytochrome b ( cytb ) was used for the genetic characterization of bird haemosporidian species [ 7 , 17 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterization of Haemoproteus tinnunculi from falcons in Saudi Arabia

2020

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“…has been reported in pigeons from Iran. For example, Tavassoli et al, (2017) examined 93 blood samples of pigeons for Haemoproteus spp. infection.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and new histopathological aspects of Haemoproteus spp. in pigeons from Iran

Bahrami

Esmaeilzadeh

Alborzi

et al. 2020

J Hellenic Vet Med Soc

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Haemoproteus spp. is pathogenic protozoan that effecting blood circulatory system of birds. The present study was undertaken to evaluate the presence of Haemoproteus spp. in pigeons from Iran and associated histopathological changes. A total of 108 blood samples were taken from pigeons to investigate Haemoproteus spp. presence by blood smear and semi-nested PCR targeting the cytochrome b gene methods. Also, to evaluate histopathological changes 12 infected pigeons to Haemoproteus were sacrificed and studied. 34.2% of pigeons infected with Haemoproteus showed macro and microgametocytes in their erythrocytes while based on the molecular method 63.8% were infected. Focal lymphocytic aggregates, pigmentation and cell swelling were the main histopathological lesions in infected livers. Multifocal non-suppurative interstitial nephritis, pigmentation and splenic lymphoid hyperplasia were also seen in the infected pigeons. Mild lymphocytic myocarditis in the heart of one pigeon was the other finding. No histopathological changes were seen in brain, intestine, and pancreas. Schizonts with variable shapes and sizes were detected in infected livers, lungs, kidneys, and spleens but megaloschizonts were not found. This study also reports the molecular prevalence of Haemoproteus spp. in Iran

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PCR-RFLP detection of Haemoproteus spp. (Haemosporida: Haemoproteidae) in pigeon blood samples from Iran

Cited by 7 publications

References 0 publications

Detection of haemosporidian parasites in wild and domestic birds in northern and central provinces of Iran: Introduction of new lineages and hosts

Detection of haemosporidian parasites in wild and domestic birds in northern and central provinces of Iran: Introduction of new lineages and hosts

Prevalence and molecular characterization of Haemoproteus tinnunculi from falcons in Saudi Arabia

Prevalence and new histopathological aspects of Haemoproteus spp. in pigeons from Iran

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