PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women

Frej−Mądrzak, Magdalena; Gryboś, Anna; Gryboś, Marian; Teryks−Wołyniec, Dorota; Jama-Kmiecik, Agnieszka; Sarowska, Jolanta; Choroszy−Król, Irena

doi:10.5603/gp.a2018.0020

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…One could speculate that the manufacturer’s primer design might disfavor the detection of specific ompA sequences, which were frequently included in our study. Considerably, other commercially available PCRs target more than one genomic region, e.g., a sequence located on a cryptic plasmid combined with ompA or the 16S rRNA gene 33 , 34 . This might explain a higher sensitivity of other assays despite the low EA limit of only 20 geq/reaction specified by the manufacturer.…”

Section: Discussionmentioning

confidence: 99%

A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents

Dichtl¹,

Osterman²,

Förster³

et al. 2023

Sci Rep

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With an incidence of more than > 1,000,000/day, sexually transmitted diseases remain a major challenge for health care systems worldwide. To reduce disease burden, complications, and spread, rapid diagnosis permitting early therapy is pivotal. The range of pathogens is wide and co-infections are common. This complicates pre-analytics, which are based on different laboratory techniques with potentially long turnaround times, e.g., cultivation and multistep serologies. Multiplex PCR provides the opportunity to overcome these limitations. In this study, we evaluated a novel assay, the Euroarray STI-11 microarray (EA; Euroimmun Medizinische Labordiagnostika), for the detection of eight obligate or facultative pathogens. Three-hundred-thirteen clinical specimens, which had been tested and pre-characterized for STI causing agents as part of routine diagnostics, were used as cases and controls in this retrospective study. The EA detected 34/44 Chlamydia trachomatis, 48/50 HSV-1, 50/50 HSV-2, 48/48 Mycoplasma hominis, 45/47 Neisseria gonorrhoeae, 9/11 Treponema pallidum, 46/46 Ureaplasma parvum, and 49/49 Ureaplasma urealyticum infections, respectively. 293 samples were EA positive, with polymicrobial infections (positive for two to six microbial or viral agents) detected in 130/293 cases. Specificities were 100% in the respective control groups (n = 18–48 depending on targeted pathogen) except for N. gonorrhoeae (25/26) and U. urealyticum (44/45). The broad spectrum of obligate and facultative pathogens targeted by the EA makes it a valuable tool in the setting of STI diagnostics and surveillance. The test has the potential to diagnose diseases neglected or overlooked in routine clinical practice. Besides a low sensitivity for C. trachomatis, the EA demonstrated high performance for all analyzed parameters. Further studies are warranted in order to capture a larger variety of the tested pathogens.

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Section: Discussionmentioning

confidence: 99%

A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents

Dichtl¹,

Osterman²,

Förster³

et al. 2023

Sci Rep

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show abstract

“…Como se dijo anteriormente, con el diagnostico serológico se identificaron 25 muestras con anticuerpos IgG anti- C. trachomatis ; sin embargo, con la PCR solo fue posible corroborar la infección en 21 de las mismas. La mayor proporción de muestras positivas por técnica diagnóstica inmunoenzimática pudo deberse a reactividad cruzada con C. pneumoniae o C. psittaci , porque los epítopos específicos para el género están presentes en todos los antígenos que se derivan de estas bacterias, a lo que se suma la persistencia de anticuerpos (IgG) por largos periodos y su lenta declinación 18 , 25 , 26 .…”

Section: Discussionunclassified

“…Después del último ciclo, se hizo una etapa final de 10 minutos a 72 °C para completar la elongación. Los productos de amplificación se visualizaron y fotografiaron bajo luz ultravioleta después de la electroforesis durante 45 minutos a 100 V en un gel de agarosa al 1 % con bromuro de etidio 18 , 19 .…”

Section: Materiales Y Métodosunclassified

Detección de infección por Chlamydia trachomatis en mujeres sexualmente activas en Venezuela

et al. 2022

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Introducción. La infección genital por Chlamydia trachomatis es una de las más frecuentes en el mundo. Cada año se registran cerca de 85 millones de nuevos casos de esta enfermedad, que cursa con graves complicaciones en la mujer y recién nacido.Objetivo. Determinar las características clínico-epidemiológicas de la infección por C. trachomatis en mujeres venezolanas sexualmente activas.Materiales y métodos. Es un estudio descriptivo, transversal y de campo, sustentado en la historia clínica y el examen físico, la detección de infección con la prueba inmunoenzimática con anticuerpos policlonales anti-LPS y la confirmación de los resultados con la de biología molecular. La muestra estuvo conformada por 100 mujeres sexualmente activas mayores de 12 años de edad, del estado Carabobo, Venezuela.Resultados. La mayoría de las mujeres se encontraba entre los 20 y los 45 años de edad. En el 25 % de las mismas, se detectaron anticuerpos IgG anti-C. trachomatis y, en el 84 % de estas, se confirmó la infección mediante PCR; en ninguna de las mujeres se hallaron anticuerpos IgM anti-C. trachomatis.Conclusión. La infección crónica predomina en las mujeres entre los 20 y los 45 años de edad; la prueba inmunoenzimática arrojó falsos positivos corroborados por PCR.

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“…In the majority of cases, chlamydial infection is asymptomatic [ 32 ]. Nevertheless, symptoms could be felt in distinct anatomical regions with different intensities, depending on the bacterium serovar, which is determined based on the specific epitopes of the MOMP encoded by ompA [ 33 , 34 , 35 ].…”

Section: Clinical Presentation Of Chlamydial Infectionmentioning

confidence: 99%

Therapeutic Options for Chlamydia trachomatis Infection: Present and Future

et al. 2022

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Sexually transmitted infections (STIs), such as Chlamydia trachomatis (Ct) infection, have serious consequences for sexual and reproductive health worldwide. Ct is one of the most common sexually transmitted bacterial infections in the world, with approximately 129 million new cases per year. C. trachomatis is an obligate intracellular Gram-negative bacterium. The infection is usually asymptomatic, notwithstanding, it could also be associated with severe sequels and complications, such as chronic pain, infertility, and gynecologic cancers, and thus there is an urgent need to adequately treat these cases in a timely manner. Consequently, beyond its individual effects, the infection also impacts the economy of the countries where it is prevalent, generating a need to consider the hypothesis of implementing Chlamydia Screening Programs, a decision that, although it is expensive to execute, is a necessary investment that unequivocally will bring financial and social long-term advantages worldwide. To detect Ct infection, there are different methodologies available. Nucleic acid amplification tests, with their high sensitivity and specificity, are currently the first-line tests for the detection of Ct. When replaced by other detection methods, there are more false negative tests, leading to underreported cases and a subsequent underestimation of Ct infection’s prevalence. Ct treatment is based on antibiotic prescription, which is highly associated with drug resistance. Therefore, currently, there have been efforts in line with the development of alternative strategies to effectively treat this infection, using a drug repurposing method, as well as a natural treatment approach. In addition, researchers have also made some progress in the Ct vaccine development over the years, despite the fact that it also necessitates more studies in order to finally establish a vaccination plan. In this review, we have focused on the therapeutic options for treating Ct infection, expert recommendations, and major difficulties, while also exploring the possible avenues through which to face this issue, with novel approaches beyond those proposed by the guidelines of Health Organizations.

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PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women

Abstract: The study confirmed asymptotic infection in 4% of women. In own research it was not possible to confirm cor-relation between the presence of Chlamydia trachomatis and the number of partners or the number of sexual intercourses.

Cited by 6 publications

References 14 publications

A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents

A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents

Detección de infección por Chlamydia trachomatis en mujeres sexualmente activas en Venezuela

Therapeutic Options for Chlamydia trachomatis Infection: Present and Future

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