PCR detection of human papillomavirus of the mucosa: comparison between MY09/11 and GP5+/6+ primer sets

Remmerbach, Torsten Wilhelm; Brinckmann, Ute; Hemprich, Alexander; Chekol, M.; Kühndel, K; Liebert, Uwe G.

doi:10.1016/j.jcv.2003.12.011

Cited by 117 publications

(98 citation statements)

References 23 publications

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“…This study found that the HPV-positive tumor rate is 40.4%, higher than other reported studies for oral cancer (Yang et al, 2004). One possible explanation could be the sensitive PCR assay that was adopted in our study to overcome this specific problem (Remmerbach et al, 2004). As previously reported, this study confirmed that HPV16 and HPV18 were the two most prevalent HPV types (Syrjänen, 2005).…”

Section: Discussionsupporting

confidence: 82%

“…For HPV DNA detection, nested PCR (using two pairs of primers: MY09/MY11 and GP5 + /GP6 + ) was performed as described elsewhere (Remmerbach et al, 2004) with smart changes. Three controls were used for each sample described as blank control (containing all reagents except the template DNA), negative control (containing DNA purified cultures of HPV-negative cell line Wi38) and positive control (containing DNA purified cultures of HeLa cells infected with HPV18).…”

Section: Tissue Specimen Preparation and Pcr-based Hpv Typingmentioning

confidence: 99%

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Human Papillomavirus as an Independent Predictor in Oral Squamous Cell Cancer

Zhao

Chen

et al. 2009

Int J Oral Sci

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AimThere is an increasing evidence for the role of high risk human papillomavirus (HPV) in the pathogenesis of oral squamous cell carcinoma (OSCC). The purpose of this study is to evaluate the relevance of HPV infection to the survival and prognosis of OSCC. Methodology Fifty-two patients with OSCC were followed from 4 to 88 months with a median of 50. Conclusion HPV infection can act as an independent predictor for the survival and prognosis of OSCC.

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Section: Discussionsupporting

confidence: 82%

Section: Tissue Specimen Preparation and Pcr-based Hpv Typingmentioning

confidence: 99%

Human Papillomavirus as an Independent Predictor in Oral Squamous Cell Cancer

Zhao

Chen

et al. 2009

Int J Oral Sci

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“…Over the past years, various studies have investigated the role of HPVs in OSCC; but the results are highly controversial. So far, more than 130 different viral genotypes infecting the skin and mucosa have been described [2]. Most studies have indicated HPV-16 as the most prevalent HPV type in both oral and genital tumors [3].…”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus in oral squamous cells carcinoma in a population of 75 Brazilian patients

Soares

Oliveira

Souza

et al. 2007

American Journal of Otolaryngology

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Purpose: In the present study, we investigated the presence of human papillomavirus (HPV) DNA and viral types in 75 cases of oral squamous cells carcinoma from Brazil to obtain data that would contribute to a better understanding of the role of HPV in the pathogenesis of this tumor. Materials and methods: DNA was extracted from paraffin-embedded tissue and amplified by polymerase chain reaction using a pair of primers designated PCO3+ and PCO4+ for the detection of a fragment of the human h-globin gene, followed by polymerase chain reaction for the detection of HPV DNA using a pair of generic primers, GP5+ and GP6+. Viral typing was performed by dot blot hybridization.Results: Human papillomavirus DNA was detected in 18 (24%) of the 75 cases positive for the human h-globin gene. No significant association was observed between HPV and age, sex, or anatomical location of the tumor. The most prevalent viral type was HPV-18 (77,8%). Conclusion:The low frequency of detection of HPV DNA in oral epidermoid carcinomas suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. D

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“…The primers generally used in HPV DNA researches are MY09/MY11 21 , GP5/GP6 24 or GP5+ / GP6+ 22 . PCR with consensus primers M09/MY11 has shown to be less sensitive compared to the PCR in two stages (nPCR) [25][26][27] , employed in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…PCR with consensus primers M09/MY11 has shown to be less sensitive compared to the PCR in two stages (nPCR) [25][26][27] , employed in the present study.…”

mentioning

confidence: 99%

Human papillomavirus (HPV) detection in lip squamous cell carcinoma: correlation with clinical aspects and risk factors

Demathé

Garcia

Mattar³

et al. 2011

Rev. bras. epidemiol.

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. According to IARC 5 lip cancer incidence in Brazil for 2007 ranging of 0.8/100,000 (Brasília) to 1.4/100,000 (São Paulo) for man and ranging 0.1/100,000 (Cuiabá) to 0.5/100,000 (Goiânia) for woman.The highest prevalence of lip SCC occurs between the sixth and seventh decades of life. The lower lip is the oral site more affected by SCC and usually diagnosed in stage I 7 . South of Australia is among the regions with the highest incidence of lip SCC, with 13.49 cases/100,000 inhabitants/year in men and 3.21 cases/100,000 inhabitants/year in women. In Europe, specifically in Spain, lip SCC represents a major impact on men (12.70 cases/100,000 inhabitants/year), and in Switzerland presents the highest incidence in women (0.83 cases/100,000 inhabitants/ year) 8 . In France, the lip, mouth and pharynx cancer incidence reaches 38.5/100,000 9 . In Brazil, according to the estimate of National Institute of Cancer for 2008, the incidence of oral cancer was 14.45/100,000 in men and 4.83/100,000 in women non-smokers and non-drinkers suggesting that other factors, including certain viruses, may have implications on the oral carcinogenesis 11,12 . Among the viruses that appear to be related to oral cancer etiology is the human papillomavirus (HPV) [11][12][13] . HPV role in the etiology of cervix carcinoma is clear, however its participation on carcinogenesis in oral cavity and other anatomical sites such skin, esophagus, paranasal conjunctiva, bronchus, larynx, oropharynx only is suggested 13 . Syrjänen and cols. described in 1983 14 a possible link between HPV and oral cancer due to the observation of cytopathic alterations in oral lesions similar to those typically induced by HPV previously found in cervix cancer. Regarding the significance of HPV DNA presence in prognosis of patients with oral SCC, literature data are controversial [15][16][17][18] . However, its has been suggested differences between patients infected and no infected with HPV in relation to sex, age, tumor regions, histological grade, regional metastasis, rate of recurrence and survival 18. Due to the great controversy in the literature, further studies are needed to help clarify the remaining questions that still persist. The aim of this study was to investigate HPV DNA presence in biopsies of lip SCC and correlate with clinical aspects and risk factors. Materials and MethodsThis study was reviewed and approved by Research Ethics Committee of São Paulo State University -Dentistry School of Araçatuba. Thirty-three caucasian patients presenting lip SCC confirmed by histological diagnosis participated in the study.A questionnaire was completed at the initial patient trial asking about age, sex, occupation, tobacco and alcohol consumption and sun exposure. If the patient had oral cancer and none of this risk factors above the risk factor is described as "other risk factors". Smokers were considered those that smoked regularly during any time of life, even those who stopped. Non-smokers were those who never used tobacco. The same criteria were used f...

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PCR detection of human papillomavirus of the mucosa: comparison between MY09/11 and GP5+/6+ primer sets

Cited by 117 publications

References 23 publications

Human Papillomavirus as an Independent Predictor in Oral Squamous Cell Cancer

Human Papillomavirus as an Independent Predictor in Oral Squamous Cell Cancer

Human papillomavirus in oral squamous cells carcinoma in a population of 75 Brazilian patients

Human papillomavirus (HPV) detection in lip squamous cell carcinoma: correlation with clinical aspects and risk factors

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