PCR-detection of Helicobacter pylori from oral mucosa: A feasible early diagnostic tool

Jara, M.; Benso, Bruna; Lagos, María José; Carrasco, Patricio; Paulino, María Beatriz; Inostroza, Carolina

doi:10.1016/j.anndiagpath.2022.152022

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…Infections caused by Helicobacter pylori are linked to almost half of the cases of gastric body or antrum tumors [ 24 ]. The evolution of gastric cancer is gradual, moving from preneoplastic alterations (atrophic gastritis and intestinal metaplasia) before shifting to a malignant state [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…The key instigator for this transformative process is the Helicobacter pylori bacterial infection [ 25 ]. The removal of H. pylori infection has been proven to enhance the prognosis of peptic ulcer disease (PUD) and potentially gastric cancer [ 24 ]. Those affected by H. pylori infection undergo inflammation, which can lead to the formation of ulcers, Mucosa Associated Lymphoid Tissue lymphoma (MALT), and gastrointestinal system adenocarcinoma [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Unpacking the Racial Gap: Helicobacter pylori Infection Clearance Among Different Racial Groups

Rammohan,

Magam,

Joy

et al. 2023

Cureus

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IntroductionHelicobacter pylori (H. pylori) is a bacteria causing chronic stomach infections, influenced by various factors, including host traits and bacterial virulence. It uses both urease-dependent and independent mechanisms to survive acidic gastric environments. Management entails diagnosis, treatment, and eradication verification. Combining drugs is key to overcoming resistance and ensuring bacteria elimination, thus preventing recurrence and complications. H. Pylori eradication mitigates gastric cancer risk and alleviates symptoms. Racial disparities persist despite declining H. pylori and gastric cancer incidence in the United States (US). African Americans (AA) have higher gastric cancer risks than non-Hispanic Whites. Addressing these disparities is crucial to protect high-risk populations. MethodsThis study retrospectively compiled H. pylori infection data from 2009 to 2022, categorized by race. Propensity score matching balanced initial group characteristics before analysis. Chi-squared and odds ratio tests were used on the cohort, with Kaplan Meier and Log Rank methods evaluating disease clearance in ethnic groups. Data were extracted from the Sunrise Electronic Medical Record software, including patient demographics, health details, and treatment specifics. Patients aged 18-65 with H. pylori infection at Nassau University Medical Center, who followed their treatment, were selected. Data were processed using Statistical Package for the Social Sciences (SPSS) and RStudio software. ResultsThe study initially included 10,040 H. pylori-diagnosed patients, with 9,288 meeting the study's criteria after attrition. Predominantly female (64.7%), the cohort was racially diverse. A longer disease clearance time was noted among Hispanics (p=0.044). Binomial logistic regression analysis identified influential factors like high school graduation rates, poverty level income, and language proficiency on disease clearance. An odds ratio analysis further emphasized language barriers (HR 0.346, p=0.043) and education status (HR 0.756, p=0.025) as primary covariates impacting disease clearance, underlining the role of socio-economic factors and language proficiency in health outcomes. ConclusionThe study highlights racial disparities in H. pylori clearance rates, particularly among Hispanics, necessitating culturally sensitive interventions. It advocates for improved diagnostics, increased healthcare access, and social determinants of health-focused initiatives. It identifies socio-economic status and language proficiency as key factors impacting health outcomes, calling for actions to bridge these disparities. Addressing these differences can decrease healthcare inequalities and economic burden, improving overall health outcomes and reducing costs associated with H. pylori clearance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unpacking the Racial Gap: Helicobacter pylori Infection Clearance Among Different Racial Groups

Rammohan,

Magam,

Joy

et al. 2023

Cureus

View full text Add to dashboard Cite

show abstract

“…As a consequence, this increases the chances of false positive results. In addition, histology allows visualization of pathogen-induced changes in gastric tissues, such as the intensity of inflammatory cell infiltration or aberrations in gastric topology, while PCR detects the presence of genomic DNA of H. pylori in gastric tissue samples [57]. However, it should be noted that neither histological observations nor negative PCR results rule out the absence of an infection [58].…”

Section: Clinical Detection Of H Pylori Infection Generally Involves ...mentioning

confidence: 99%

Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens againstHelicobacter pylori

Das,

Halder,

Banerjee

et al. 2023

Preprint

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Chronic gastritis is one of the major symptoms of gastro-duodenal disorders typically induced byHelicobacter pylori(H. pylori).To date, no suitable model is available to study pathophysiology and therapeutic measures accurately. Here, we have presented a successful surgical infection model ofH. pylori-induced gastritis in C57BL/6 mice that resembles features similar to human infection. The proposed model does not require any preparatory treatment other than surgical intervention. C57BL/6 mice were injected with wild-type SS1 (Sydney strain 1, reference strain) directly into the stomach. Seven days post infection, infected animals showed alterations in cytokine responses along with inflammatory cell infiltration in the lamina propria, depicting a prominent inflammatory response due to infection. To understand the immunogenicity and protective efficacy, the mice were immunized with outer membrane vesicles (OMVs) isolated from an indigenous strain with putative virulence factors ofH. pylori[A61C (1),cag+/vacA s1m1]. In contrast to the nonimmunized cohort, the OMV-immunized cohort showed a gradual increase in serum immunoglobulin(s) levels on the 35thday after the first immunization. This conferred protective immunity against subsequent challenge with the reference strain (SS1). Direct inoculation ofH. pyloriinto the stomach influenced infection in a short time and, more importantly, in a dose-dependent manner, indicating the usefulness of the developed model for pathophysiology, therapeutic and prophylactic studies.

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Expression and purification of epitope vaccine against four virulence proteins from Helicobacter pylori and construction of label-free electrochemical immunosensor

Du,

Zhang,

Qiao

et al. 2023

Biosensors and Bioelectronics

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PCR-detection of Helicobacter pylori from oral mucosa: A feasible early diagnostic tool

Cited by 6 publications

References 17 publications

Unpacking the Racial Gap: Helicobacter pylori Infection Clearance Among Different Racial Groups

Unpacking the Racial Gap: Helicobacter pylori Infection Clearance Among Different Racial Groups

Establishment of an intragastric surgical model using C57BL/6 mice to study the vaccine efficacy of OMV-based immunogens againstHelicobacter pylori

Expression and purification of epitope vaccine against four virulence proteins from Helicobacter pylori and construction of label-free electrochemical immunosensor

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