PCR detection of Campylobacter sp. from turkey carcasses during processing plant in Iran

Rahimi, Ebrahim; Momtaz, Hassan; Bonyadian, Mojtaba

doi:10.1016/j.foodcont.2009.10.009

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…A high prevalence (96.7%) of thermophilic Campylobacter was noticed in modern slaughterhouse D. In this processing plant; neck skin could be contaminated either indirectly or directly. However, various studies have considered that the scalding water represents a significant source of contamination [ 8 , 28 ], but, in our study, where turkey lot was processed just after processing broiler lot, the scalding step may have prevented cross-contamination between flocks. Indeed, scald temperature was at 60°C, and according to Sanchez et al .…”

Section: Discussionmentioning

confidence: 90%

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Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys

Bouhamed¹,

Bouayad²,

Messad³

et al. 2018

Vet World

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Aim:Sources of contamination, prevalence, and antimicrobial susceptibility of thermophilic Campylobacter isolated from turkey samples were determined.Materials and Methods:A total of 300 samples were collected from 3 farms (fecal droppings) and 4 poultry slaughterhouses (neck skins and ceca) located in the middle area of Algeria (Algiers, Boumerdès, and Bouira). After detection, an antibiogram was realized only for slaughterhouses samples.Results:Samples from cecum (90.0%, 90/100; 95% confidence interval (CI)=84.1-95.9%), fecal dropping (68.0%, 68/100; 95% CI=58.9–77.1%), and neck skin (55.0%, 55/100; 95% CI=45.2–64.8%) were positive for thermophilic Campylobacter (p<0.05). Contamination rate of turkey carcasses was higher in modern slaughterhouse (96.7%) than in traditional slaughterhouses (37.1%) (p<0.05). Isolated strains were resistant to nalidixic acid (NA) (87.5%), tetracycline (TE) (81.3%), ciprofloxacin (CIP) (75.0%), ampicillin (AM) (65.6%), and erythromycin (25.0%) (p<0.05). 96.9% (124/128) of the isolates were multiresistant and 18 drug resistance patterns were registered. The predominant one (43.0%) was AM, NA, CIP, and TE.Conclusions:Potential sources of contamination of this fastidious bacterium were noticed in farms and slaughterhouses. Modern slaughterhouse allowed contamination of turkey carcasses more than a traditional slaughterhouse. However, the scalding step could not represent a source of contamination. The most tested strains exhibited resistance to erythromycin and/or CIP. It is worrisome because these molecules are considered as first-choice antibiotics for human campylobacteriosis.

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Section: Discussionmentioning

confidence: 90%

“…Indeed, they were isolated from human fecal samples with a rate of 17.7% [ 7 ]. In developed countries, thermophilic Campylobacter isolation rates ranged from 55 to 77% in turkey samples [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys

Bouhamed¹,

Bouayad²,

Messad³

et al. 2018

Vet World

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“…Poultry plays an important role in transmission of this foodborne pathogen. In particular, chicken and turkey carcasses have been reported to be contaminated with rates up to 100% and 62.1%, respectively (16,24). Contamination and subsequent colonization of poultry flocks on the farm often lead to transmission of Campylobacter along the whole production chain (19).…”

Section: Praca Oryginalnamentioning

confidence: 99%

Prevalence and antimicrobial resistance of Campylobacter isolates from poultry offals

Wysok¹,

Wojtacka²,

Łaszczych³

et al. 2017

Medycyna Weterynaryjna

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SummaryThe aim of the study was to determine the presence of Campylobacter strains in poultry by-products and define antimicrobial resistance of isolates. In total, 400 samples were tested among which 300 included the liver, heart and stomach, and 100 samples represented the contents of the cecum. The samples were taken from chickens and turkeys in the slaughterhouse after evisceration. The prevalence of Campylobacter in chicken samples was 100% with regards to the contents of cecum and offal. The turkey origin Campylobacter strains were noted in 76% of the livers, 78% hearts and 82% gizzards. The samples of cecum contents were positive in 60%. Species analysis of the strains isolated showed C. jejuni as dominant. The estimation of sensitivity to antibiotics showed that Campylobacter strains were most frequently resistant to quinolones and tetracyclines. Resistance to ciprofloxacin was detected among 52.7% and 52.5% chicken and turkey origin strains. The same was noted regarding nalidixic acid, resistance to which was shown in 56% and 58.5% isolates, respectively. Regarding tetracyclines, the highest resistance of the strains from chicken and turkey was detected to doxycyclinum in 61.3% and 53.3% of isolates, respectively. However the highest sensitivity was showed to erythromycin, gentamicin and chloramphenicol. Only one C. coli strain from turkey offal was resistant to gentamicin. Simultaneously multi drug resistance was defined. The aimed studies showed that 62% of C. jejuni and 53.8% of C. coli strains from chicken offal were resistant to two or more agents. In turkey origin isolates MDR was detected in 54.7% of C. jejuni and 53.3% of C. coli strains.

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“…Consumption of contaminated food (mainly poultry), undercooked meat, unpasteurized milk and contaminated water are the most common mode of transmission. Contamination during food preparation has also been reported in some studies (Rahimi et al 2010(Rahimi et al , 2017Ommi et al 2017). Infection with C. jejuni and C. coli may be asymptomatic or associated with some non-specific clinical signs such as diarrhoea, weight loss and anorexia (Hakkinen et al 2007).…”

Section: Introductionmentioning

confidence: 95%

“…Contamination during food preparation has also been reported in some studies (Rahimi et al . , ; Ommi et al . ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of thermotolerant Campylobacter species in dogs and cats in Iran

Torkan

Vazirian

Khamesipour

et al. 2018

Veterinary Medicine & Sci

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Campylobacter is considered the most common bacterial cause of human gastroenteritis in the world with C. jejuni being regarded as the primary cause of bacterial gastroenteritis. A broad range of other Campylobacter species, including C. coli have also been implicated in human gastroenteritis. This study sought to isolate, characterize and assess the antibiogram of Campylobacter jejuni and C. coli from faecal samples obtained from cats and dogs in Isfahan and Shahrekord cities in Iran. Faecal samples were collected from 100 pets comprising of 50 dogs and 50 cats from March 2015 to March 2016; incorporating the four seasons (spring, summer, autumn and winter). Campylobacter spp. was isolated by culture, characterized by biochemical tests and confirmed by PCR‐based assays. Antimicrobial susceptibility test was performed by the Kirby–Bauer disk diffusion method, using Mueller Hinton agar. A total of 19 Campylobacter isolates among them two C. jejuni and one C. coli were recovered from dogs and cats’ faecal samples. The prevalence rates of Campylobacter spp. were 16.0% (8 out of 50) in dogs and 22.0% (11 out of 50) in cats. The highest (4 out of 16, 25%) Campylobacter spp. prevalence among dogs was reported in autumn and the lowest (1 out of 11, 9.1%) in spring, while among the cats, the highest (4 out of 12, 33.3%) Campylobacter spp. prevalence was reported in summer and lowest (1 out of 11, 9.09%) in spring. Campylobacter spp. isolated from faecal samples obtained from cats and dogs exhibited the most frequent antimicrobial resistance against tetracycline at 81.8% and 87.5%, respectively, compared to all other antimicrobial agents. These results show a low prevalence of Campylobacter spp. in faecal samples obtained from pet dogs and cats in Shahrekord and Isfahan cities in Iran. Given the relatively low prevalence of the C. jejuni and C. coli in pet dogs and cats in Isfahan and Shahrekord cities, it can be assumed that their importance as reservoirs for infection in humans is likely to be limited to the studied cities, but should not be neglected.

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PCR detection of Campylobacter sp. from turkey carcasses during processing plant in Iran

Cited by 13 publications

References 24 publications

Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys

Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys

Prevalence and antimicrobial resistance of Campylobacter isolates from poultry offals

Prevalence of thermotolerant Campylobacter species in dogs and cats in Iran

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