PCR detection and serotyping of enterotoxigenic and shigatoxigenic Escherichia coli isolates obtained from chicken meat in Mumbai, India

Abstract

Aim: Present study was undertaken to find out the frequency of few virulent genes and prevalence of related strains of Escherichia coli isolated from chicken meat obtained from chicken retail shops by Polymerase Chain Reaction (PCR).Materials and Methods: 66 samples of freshly slaughtered chicken meat were collected from 22 identified retail shops located at Mumbai city, randomly. Processed meat samples were cultured in EMB agar and presumptive colonies were confirmed by various biochemical tests. PCR method was accustomed for identification of the genes coding for heat-stable enterotoxin a (STa), heat labile enterotoxin (LT), shiga-like toxins 1 and 2 (SLT1 and SLT2). E. coli isolates were sent to National Salmonella and Escherichia Centre, CRI, Kasauli, HP, India for serotyping.Results: 11 (16.67%) E. coli strains were isolated from 66 chicken meat samples. 3 (27.27%) out of 11 harbored the gene for SLT2, and 2 (18.18%) for STa. None of the strain contains SLT1 and LT genes. Serotypes detected were rough, O2, O20, O22, O102 each for one isolate and 6 isolates were untypable (UT). Conclusion:The results concluded that chicken meat samples analysed harbored genes for shiga like toxins and enterotoxins and different serotypes of E. coli. These findings indicating that regular monitoring of chicken meat is essential for this pathogen to prevent potential public health problems.

Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

“…O26 reported the highest serotype present. This result was nearly similar to results of Kudakwashe et al (2013) and agree with Zende et al (2013) in O2 serotype only.…”

“…Six out of the 50 chicken samples investigated for the presence of E. coli by percentage 12%. The prevalence of E. coli in chicken samples 12% was nearly similar with that was reported by Momtaz et al (2012) and Zende et al (2013) who reported that the incidence of E.coli in chicken meat was 11% and 16.67 %, respectively. On the other hand, the present results were lower than those reported by Hyun-jung et al (2015) and Nguyen et al (2016) who reported E.coli presented in chicken meat with percentage 75.9% and 92.7%, respectively.…”

“…Lower percent of STEC isolated by Abdul Razzaq et al (2013) was 2 % of chicken meat. Stx2 percent which detected in this study (83%) was higher than that recorded by Panahee and Pourtaghi (2016) and Zende et al (2013) which was 21%, 27% respectively. The present data showed that STEC results in raw meat 45% Stx1; 36% Stx2 and 36% eae A.…”

Uji Efektifitas Ekstrak Daun Mimba terhadap Micrococcus luteus yang Diisolasi dari Anjing Penderita Dermatitis Kompleks

“…(Jiang et al, 2005). Bakteri E. coli ditemukan dengan jumlah yang melimpah pada air dengan kualitas yang rendah (Zende et al,2013). Bakteri E. coli disebut juga koliform fekal, hal ini karena E.coli ditemukan di dalam saluran usus ternak dan manusia dan didapatkan di dalam feses, sehingga E. coli dikenal sebagai indikator kontaminasi kotoran (Allocati et al, 2013).…”

Virulence Factors and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Ogun River, Abeokuta Metropolis, Nigeria

“…Based on the production of virulence factors and their toxigenicity, E. coli can be grouped into six classes. These are Enterohemorrhagic E. coli (EHEC), Enterotoxigenic E. coli (ETEC), Enteropathogenic E. coli (EPEC), Enteroinvasive E. coli (EIEC), Enteroaggregative E. coli (EAggEC), and Diffusely adherent E. coli (DAEC) [3].…”