PCR: Clinical Diagnostics and Research

Rolfs, Arndt; Schuller, Irmela; Finckh, Ulrich; Weber-Rolfs, Ines

doi:10.1007/978-3-642-77492-8

Cited by 105 publications

(90 citation statements)

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“…Positive controls contained 80 ng B. abortus 2308 DNA as template, and negative controls consisted of sterile water instead of DNA template. Generally recommended procedures were used to avoid contamination (Rolfs et al, 1992), and each sample was tested at least in triplicate. with sera taken before immunizations showed no reactivity in any of the immunological tests.…”

Section: Serological Analysesmentioning

confidence: 99%

Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.

Velasco

Romero

López-Goñi

et al. 1998

International Journal of Systematic Bacteriology

186

151

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Evaluation of the relatedness ofThe relatedness of Brucella spp. and Ochrobactrum anthropi was studied by protein profiling, Western blot, immunoelectrophoresis and 16s rRNA analysis. Whole-cell and soluble proteins of brucellae and 0. anthropi showed serological cross-reactivities quantitatively and qualitatively more intense than those existing with similar extracts of Agrobacterium spp. Numerical analysis of Western blot profiles of whole-cell extracts showed that 0. anthropi LMG 3301 was closer to Brucella spp. than to 0. anthropi LMG 3331T, a result not obtained by protein profiling. These differences were not observed by Western blot with soluble fractions, and immunoelectrophoretic analyses suggested that this was due to destruction of conformational epitopes in Western blot procedures with the subsequent simplification of antigenic profile. Analysis of the 165 rRNA sequences of strains previously used in the species definition confirmed that strain LMG 3301, and also LMG 3306, were closer to the brucellae, and that LMG 3331T was in a separate cluster. The LMG 3301 and the LMG 3331T clusters could also be separated by their different colistin sensitivity and by PCR with 16s rRNA Brucella primers, and both methods showed strains of both clusters among clinical isolates classified as 0. anthropi by conventional tests. These results and those of previous DNA-DNA hybridization studies [Holmes, B. , Popoff, M., Kiredjian, M. & Kersters, K. (1988). Int J Syst Bacterial 38,4064161 show that the LMG 3301 cluster and related clinical isolates should be given a new species status for which the name Ochrobactrum intermedium sp. nov. is proposed (type strain is LMG 3301T = NCTC 12171' = CNS 2-75').

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Section: Serological Analysesmentioning

confidence: 99%

Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.

Velasco

Romero

López-Goñi

et al. 1998

International Journal of Systematic Bacteriology

186

151

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“…Rapid amplification of cDNA ends (5' RACE) (Rolfs et al, 1992) was used to isolate a series of overlapping clones (KB1,2,3,6,8,and 9) encoding the NC3 domain of mouse al(XI1) collagen (see Fig. 1).…”

Section: Experimental Procedures Isolation and Sequencing Of Cdnas Enmentioning

confidence: 99%

Primary structure of the long and short splice variants of mouse collagen XII and their tissue‐specific expression during embryonic development

Böhme

et al. 1995

Developmental Dynamics

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Type XI1 collagen, a member of the FACIT group of extracellular matrix proteins, consists of molecules that are trimers of orl(XI1) chains. The three chains in each molecule form a cross-shaped structure with a central globule from which a triple-helical tail and three finger-like regions (containing von Willebrand factor A-like domains and fibronectin type I11 repeats) extend. cDNA cloninglsequencing of chicken al(XI1) collagen and protein studies with mouse, bovine, and human material suggest that the cyl(XI1) collagen gene gives rise to two molecular variants, differing in the length of the finger-like regions, by alternative splicing of the primary transcript. To provide a basis for studies of the function of the two variants in an organism that can be genetically manipulated, we have isolated and sequenced mouse cDNAs encoding both splice variants. The sequence provides the first complete nucleotide and amino acid sequence of mammalian type XI1 collagen. From these cDNAs we have generated digoxigenin-labeled RNA probes for in situ hybridization of developing mouse embryos to find out whether the splicing mechanism responsible for generation of the two forms is developmentally regulated. The results, combined with Northern blot and RT-PCR analysis of RNA from embryos at various developmental stages, demonstrate that the long form of collagen XII, XIIA, is the predominant form at early stages (ED7 and 11); at later stages of development (ED15 and 17) the short form, XIIB, becomes the major form. As the short form becomes the major product, the long splice variant continues to be expressed in several tissues, even after birth. An exception is dermis, which is positive for the long form up to embryonic day 15, but negative at day 18, when only the short form RNA can be detected. o 1995 Wiley-Liss, Inc.

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“…For the visualization of amplicons, primers of the inner PCR were labeled with 5Ј-digoxigenin (TIB Molbiol, Berlin, Germany). Appropriate positive and negative controls were included in each experiment, and precautionary measures to avoid contamination were taken as described earlier (13,15).…”

mentioning

confidence: 99%

Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

et al. 2001

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To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, Borrelia garinii and Borrelia afzelii, respectively, were exclusively detected. The results demonstrate that PCR-RLB is a valuable tool for epidemiological and pathogenetic studies of Lyme borreliosis.

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PCR: Clinical Diagnostics and Research

Cited by 105 publications

References 0 publications

Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.

Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.

Primary structure of the long and short splice variants of mouse collagen XII and their tissue‐specific expression during embryonic development

Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

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