Rapid Typing of
            <i>Borrelia burgdorferi</i>
            Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

Lünemann, Jan D.; Zarmas, Silvia; Priem, Susanne; Franz, Juliane K.; Zschenderlein, Rolf; Aberer, Elisabeth; Klein, Rolf; Schouls, Leon; Burmester, Gerd R.; Krause, Andreas

doi:10.1128/jcm.39.3.1130-1133.2001

Cited by 50 publications

(33 citation statements)

References 24 publications

(17 reference statements)

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“…Since B. burgdorferi, the spirochete responsible for this zoonotic infection, is phenotypically and genotypically heterogeneous, it causes variability in the clinical aspects of the disease. Arthritis and carditis are preferentially associated with B. burgdorferi sensu stricto, the degenerative skin disorder acrodermatitis chronica et atrophicans is primarily associated with Borrelia afzelii, and neuroborreliosis is primarily associated with Borrelia garinii (13,18). However, B. burgdorferi sensu stricto is also responsible for human neuroborreliosis in the United States (31).…”

mentioning

confidence: 99%

Identification of Three Clinically Relevant Borrelia burgdorferi Sensu Lato Genospecies by PCR-Restriction Fragment Length Polymorphism Analysis of 16S-23S Ribosomal DNA Spacer Amplicons

et al. 2004

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We report the results of a study of the prevalences of three clinically relevant Borrelia burgdorferi sensu lato genospecies (Borrelia burgdorferi sensu stricto, Borrelia afzelii, and Borrelia garinii) in 1,040 questing Ixodes ticks from all regions of Latvia, where Lyme borreliosis is endemic. The prevalences of Borrelia in Ixodes ricinus and Ixodes persulcatus were 22.6 and 27.9%, respectively. Molecular typing of B. burgdorferi from infected ticks was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments of the 16S-23S (rrs-rrlA) rRNA intergenic spacer by using species-specific primers and subsequent sequencing. The dominant Borrelia species in both Ixodes species was B. afzelii. In addition, different restriction patterns of B. garinii and B. afzelii were also identified. This study demonstrates that the 16S-23S rRNA PCR-RFLP typing method is simple, sensitive, and fast and that it allows one to differentiate among B. burgdorferi species and subspecies with various degrees of pathogenic potential directly in ticks. These features are important in monitoring Lyme disease.

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mentioning

confidence: 99%

Identification of Three Clinically Relevant Borrelia burgdorferi Sensu Lato Genospecies by PCR-Restriction Fragment Length Polymorphism Analysis of 16S-23S Ribosomal DNA Spacer Amplicons

et al. 2004

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“…The final stage is chronic Lyme borreliosis and is usually characterized by development of reoccurring arthritis in large joints as well as a continuation of neurologic manifestations (41,47). The three Borrelia genospecies known to cause disease exhibit somewhat different manifestations; for example, B. burgdorferi tends to be associated with the development of arthritis, B. garinii tends to be associated with neuroborreliosis, and B. afzelii tends to be associated with acrodermatitis chronicum atrophicans (3,29,51).…”

mentioning

confidence: 99%

Effect of Complement Component C3 Deficiency on Experimental Lyme Borreliosis in Mice

Lawrenz¹,

Wooten

Zachary

et al. 2003

Infect Immun

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Mice deficient in complement component C3 (C3؊/؊ ) and syngeneic C57BL/6 control mice were challenged with Borrelia burgdorferi to determine the role of complement in immune clearance and joint histopathology during experimental Lyme borreliosis. Tibiotarsal joint, ear, and heart tissues were monitored for spirochete numbers at 2, 4, 8, and 12 weeks postinoculation with 10 5 B. burgdorferi B31 clone 5A4 by using quantitative real-time PCR. The spirochete load in joint and ear tissue remained higher in the C3 ؊/؊ mice than in the wild-type counterparts throughout the 12-week study, whereas the numbers in heart tissue of both groups of mice decreased substantially at 8 to 12 weeks postinfection. Histopathology scores for joint tissue were generally higher in the C3 ؊/؊ mice compared to C57BL/6 controls at 2 and 4 weeks postinfection, which may reflect the presence of higher numbers of bacteria in the joints at these early time points. Levels of anti-B. burgdorferi immunoglobulin G tended to be reduced in the C3 ؊/؊ mice compared to control mice. Furthermore, a 5.5-fold-lower number of the complement-sensitive Borrelia garinii was needed to infect C3 ؊/؊ mice compared to C57BL/6 mice, indicating that its sensitivity to complement is one barrier to infection of the mouse model by B. garinii. These results indicate that the complement system may be important in controlling the early dissemination and progression of B. burgdorferi infection.

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“…During this time, Borrelia changes its outer proteins profi le and in the salivary glands binds immunosupresive Salp15 (6). "Armed" with this protein, Borrelia leaves tick (7) and enters to the human (8). the other hand, cp9 is probably a fragment of ancestral cp32.…”

Section: Genome Structurementioning

confidence: 99%

“…Neurological complications are frequently associated with infections caused by B. garinii and B. burgdorferi 6 . Among dermatological manifestations, typical are erythema, mentioned above, and acrodermatitis chronica atrophicans, which is caused mostly by B. afzelii 7,8 . Lymphocytoma and circumscribed scleroderma are less common.…”

Section: Introductionmentioning

confidence: 99%

Biological Aspects of Lyme Disease Spirochetes: Unique Bacteria of the Borrelia Burgdorferi Species Group

Křupka¹,

Raška²,

Bĕláková³

et al. 2007

BIOMED PAP

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Background: Borrelia burgdorferi sensu lato is a group of at least twelve closely related species some of which are responsible for Lyme disease, the most frequent zoonosis in Europe and the USA. Many of the biological features of Borrelia are unique in prokaryotes and very interesting not only from the medical viewpoint but also from the view of molecular biology.Methods: Relevant recent articles were searched using PubMed and Google search tools. Results and Conclusion:This is a review of the biological, genetic and physiological features of the spirochete species group, Borrelia burgdorferi sensu lato. In spite of a lot of recent articles focused on B. burgdorferi sensu lato, many features of Borrelia biology remain obscure. It is one of the main reasons for persisting problems with prevention, diagnosis and therapy of Lyme disease. The aim of the review is to summarize ongoing current knowledge into a lucid and comprehensible form.

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Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

Cited by 50 publications

References 24 publications

Identification of Three Clinically Relevant Borrelia burgdorferi Sensu Lato Genospecies by PCR-Restriction Fragment Length Polymorphism Analysis of 16S-23S Ribosomal DNA Spacer Amplicons

Identification of Three Clinically Relevant Borrelia burgdorferi Sensu Lato Genospecies by PCR-Restriction Fragment Length Polymorphism Analysis of 16S-23S Ribosomal DNA Spacer Amplicons

Effect of Complement Component C3 Deficiency on Experimental Lyme Borreliosis in Mice

Biological Aspects of Lyme Disease Spirochetes: Unique Bacteria of the Borrelia Burgdorferi Species Group

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