PCR-Based Techniques for the Detection of Plant Viruses and Viroids

Candresse, T.; Lanneau, M.; Revers, Frédéric; Kofalvi, S. A.; Macquaire, G.

doi:10.17660/actahortic.2000.530.5

Cited by 5 publications

(2 citation statements)

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“…Molecular methods like PCR (30)(31)(32)(33), Nucleic Acid Spot Hybridization (NASH) assay (34)(35)(36)(37)(38)(39)(40) depend on viral nucleic acids and viral speci c primers or probes. PCR is the most commonly used detection technique for begomoviruses and primers were primarily designed on coat protein and replicase genes (41).…”

Section: Introductionmentioning

confidence: 99%

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Ramesh

Viswanathan

2021

Preprint

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Begomoviruses are a major class of Geminiviruses that affects most dicotyledonous plants and causes heavy economic losses to farmers. Early detection of begomovirus is essential to control the spread of the disease and prevent loss. Many available detection methods like ELISA, immunosorbent electron microscopy, PCR or qPCR require expertise in handling sophisticated instruments, complex data interpretation and costlier chemicals, enzymes or antibodies. Hence there is a need for a simpler detection method, here we report the development of a visual detection method based on functionalized gold nanoparticles (AuNP probe assay). The assay was able to detect upto 500 ag/𝜇l of begomoviral DNA (pTZCCPp3, a clone carrying partial coat protein gene) suspended in MilliQ water. Screening of chilli plants for begomoviral infection by PCR (Deng primers) and AuNP assay showed that AuNP assay (77.7%) was better than PCR (49.4%). The AuNP assay with clccpi1 probe was able to detect begomoviral infection in chilli, tomato, common bean, green gram and black gram plants which proved the utility and versatility of the AuNP assay. The specificity of the assay was demonstrated by testing with total DNA from different plants that are not affected by begomoviruses.

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Section: Introductionmentioning

confidence: 99%

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Ramesh

Viswanathan

2021

Preprint

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show abstract

“…Molecular methods like PCR [30][31][32][33] , Nucleic Acid Spot Hybridization (NASH) assay [34][35][36][37][38][39][40] depend on viral nucleic acids and viral specific primers or probes. PCR is the most commonly used detection technique for begomoviruses and primers were primarily designed on coat protein and replicase genes 41 .…”

mentioning

confidence: 99%

Detection of Begomovirus in chilli and tomato plants using functionalized gold nanoparticles

Lavanya

Viswanathan

2021

Sci Rep

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Begomoviruses are a major class of Geminiviruses that affects most dicotyledonous plants and causes heavy economic losses to farmers. Early detection of begomovirus is essential to control the spread of the disease and prevent loss. Many available detection methods like ELISA, immunosorbent electron microscopy, PCR or qPCR require expertise in handling sophisticated instruments, complex data interpretation and costlier chemicals, enzymes or antibodies. Hence there is a need for a simpler detection method, here we report the development of a visual detection method based on functionalized gold nanoparticles (AuNP assay). The assay was able to detect up to 500 ag/µl of begomoviral DNA (pTZCCPp3, a clone carrying partial coat protein gene) suspended in MilliQ water. Screening of chilli plants for begomoviral infection by PCR (Deng primers) and AuNP assay showed that AuNP assay (77.7%) was better than PCR (49.4%). The AuNP assay with clccpi1 probe was able to detect begomoviral infection in chilli, tomato, common bean, green gram and black gram plants which proved the utility and versatility of the AuNP assay. The specificity of the assay was demonstrated by testing with total DNA from different plants that are not affected by begomoviruses.

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The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites

et al. 2012

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PCR-Based Techniques for the Detection of Plant Viruses and Viroids

Cited by 5 publications

References 0 publications

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Detection of Begomovirus in Chilli and Tomato Plants Using Functionalized Gold Nanoparticles Authors: Lavanya. R and Arun. V

Detection of Begomovirus in chilli and tomato plants using functionalized gold nanoparticles

The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites

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