The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites

Rodríguez, Marianela; Muñoz, Nacira; Lenardon, S. L.; Lascano, Ramiro

doi:10.1016/j.plantsci.2012.08.008

Cited by 25 publications

(28 citation statements)

References 57 publications

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“…It has been suggested that early sugar increase produced during compatible SuCMoV interaction could be modulating chlorotic symptom development in sunflower leaves through redox state alteration. 24 The present results showed that 24 hours of sugar treatments (200 mM glucose and sucrose) significantly increased endogenous levels of soluble sugars and starch, total and reduced glutathione, glutathione redox couple, total ascorbate, pyridine nucleotide, and ATP contents, all of them associated with a decrease in ΦPSII and psbA gene expression level.…”

Section: Discussionsupporting

confidence: 52%

“…38 Likewise, the significant increase in glutathione redox couple is supported by the increase in glutathione reductase activity induced by both compatible sunflower-SuCMoV interaction and sugar treatment, as previously demonstrated. 4,24 Glutathione is mainly located in chloroplasts, and highly reduced glutathione content induces photoinhibition because it increases the degree of reduction of quinone A (QA), decreasing electron transport efficiency of PSII. 39 Moreover, we found increases in pyridine nucleotide content without redox changes during sunflower-SuCMoV interaction.…”

Section: Discussionmentioning

confidence: 99%

“…16 ATP content ATP concentration was determined according to Rodríguez et al 24 using an ENLITEN ® ATP Assay System Bioluminescence Detection kit (Promega, Madison, WI).…”

Section: Pyridine Nucleotide Contentmentioning

confidence: 99%

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Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: Similarities with Sunflower chlorotic mottle virus-induced symptom

et al. 2013

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Sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, ATP and ROS. These elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many endogenous and environmental stimuli. Our previous findings suggested that early sugar increase produced during compatible Sunflower chlorotic mottle virus (SuCMoV) infection might modulate chlorotic symptom development through redox state alteration in sunflower. The purpose of this work was to characterize redox-related metabolites and gene expression changes associated with high sugar availability and symptom development induced by SuCMoV. The results show that sugar caused an increase in glutathione, ascorbate, pyridine nucleotides, and ATP. In addition, higher sugar availability reduced hydrogen peroxide and ΦPSII. This finding suggests that high sugar availability would be associated with cellular redox alteration and photoinhibitory process. The expression of the genes analyzed was also strongly affected by sugar, such as the down-regulation of psbA and up-regulation of psbO and cp29. The expression level of cytoplasmic (apx-1 and gr)- and chloroplastic (Fe-sod)-targeted genes was also significantly enhanced in sugar-treated leaves. Therefore, all these responses suggest that sugars induce chloroplastic redox state alteration with photoinhibition process that could be contributing to chlorotic symptom development during SuCMoV infection.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: Similarities with Sunflower chlorotic mottle virus-induced symptom

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“…Virus infection could cause either up- or down-regulated protein expressions in plants and lead plants’ phenotype change (Takahashi et al, 1991; Rodríguez et al, 2012). However, it is difficult to differentiate genetic variegations from those induced by plant viruses because they are morphologically similar (Valverde et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, several studies have identified the responses of virus infection in plants and show various effects on components of the OEC. These viruses reduce the expression of PsbP (Kong et al, 2014), PsbO (Rodríguez et al, 2012), or both PsbP and PsbQ (Takahashi et al, 1991; Rahoutei et al, 2000; Sui et al, 2006), along with the appearance of chlorotic spots on leaves (Takahashi et al, 1991; Rahoutei et al, 2000; Sui et al, 2006; Rodríguez et al, 2012). OEC is one of the major protein targets in plants that interact with CymMV virus (Nanda and Biswal, 2008; Kong et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant (Phalaenopsis aphrodite subsp. formosana)

Tsai

Sheue

et al. 2017

Front. Plant Sci.

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Leaf variegation is often the focus of plant breeding. Here, we studied a variegated mutant of Phalaenopsis aphrodite subsp. formosana, which is usually used as a parent of horticultural breeding, to understand its anatomic and genetic regulatory mechanisms in variegation. Chloroplasts with well-organized thylakoids and starch grains were found only in the mesophyll cells of green sectors but not of yellow sectors, confirming that the variegation belongs to the chlorophyll type. The two-dimensional electrophoresis and LC/MS/MS also reveal differential expressions of PsbP and PsbO between the green and yellow leaf sectors. Full-length cDNA sequencing revealed that mutant transcripts were caused by intron retention. When conditioning on the total RNA expression, we found that the functional transcript of PsbO and mutant transcript of PsbP are higher expressed in the yellow sector than in the green sector, suggesting that the posttranscriptional regulation of PsbO and PsbP differentiates the performance between green and yellow sectors. Because PsbP plays an important role in the stability of thylakoid folding, we suggest that the negative regulation of PsbP may inhibit thylakoid development in the yellow sectors. This causes chlorophyll deficiency in the yellow sectors and results in leaf variegation. We also provide evidence of the link of virus CymMV and the formation of variegation according to the differential expression of CymMV between green and yellow sectors.

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The detection of hydrogen peroxide involved in plant virus infection by fluorescence spectroscopy

Lei

Qiu

et al. 2016

Luminescence

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The production of reactive oxygen species (ROS) forms part of the defense reaction of plants against invading pathogens. ROS have multifaceted signaling functions in mediating the establishment of multiple responses. To verify whether hydrogen peroxide (H2 O2 ) contributes to plant virus infection and the development of induced symptoms, we used fluorescence to monitor the generation of H2 O2 and confocal laser scanning microscopy (CLSM) to investigate the subcellular distribution of H2 O2 in leaves. In this study, the M strain of Cucumber mosaic virus (M-CMV) induced heavy chlorotic symptoms in Nicotiana tabacum cv. white burley during systemic infection. Compared with mock-inoculated leaves, H2 O2 accumulation in inoculated leaves increased after inoculation, then decreased after 4 days. For systemically infected leaves that showed chlorotic symptoms, H2 O2 accumulation was always higher than in healthy leaves. Subcellular H2 O2 localization observed using CLSM showed that H2 O2 in inoculated leaves was generated mainly in the chloroplasts and cell wall, whereas in systemically infected leaves H2 O2 was generated mainly in the cytosol. The levels of coat protein in inoculated and systemically infected leaves might be associated with changes in the level of H2 O2 and symptom development. Further research is needed to elucidate the generation mechanism and the relationship between coat protein and oxidative stress during infection and symptom development. Copyright © 2016 John Wiley & Sons, Ltd.

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The chlorotic symptom induced by Sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites

Cited by 25 publications

References 57 publications

Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: Similarities with Sunflower chlorotic mottle virus-induced symptom

Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: Similarities with Sunflower chlorotic mottle virus-induced symptom

Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant (Phalaenopsis aphrodite subsp. formosana)

The detection of hydrogen peroxide involved in plant virus infection by fluorescence spectroscopy

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