PCR-based restriction fragment length polymorphism analysis of DNA sequence diversity of flagellin genes of Campylobacter jejuni and allied species

Owen, Robert J.; Fayos, A.; Hernández, Javier; Lastovica, A

doi:10.1006/mcpr.1993.1070

Cited by 23 publications

(24 citation statements)

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“…Previous studies demonstrated that strains of C. jejuni have a significant degree of diversity within some regions of the flagellin gene sequences, [13,14] and it has been suggested that these variations could provide a novel basis for discriminating and grouping isolates in epidemiological investigations [16][17][18]. The results of the present study provide further evidence of diversity within the fla gene sequences of C. jejuni with 77 strains giving 8 patterns representing 8 genotypes.…”

Section: Discussionsupporting

confidence: 68%

“…However, the majority of strains (55%) were characterized by having the F-I genotype. In a previous study [17], we described the genotypes of a set of 28 strains of C. jejuni including 15 South African clinical isolates. The correspondence between those types and types defined in the present study is shown in Table 3.…”

Section: Discussionmentioning

confidence: 99%

“…Nucleotide sequence data indicate significant divergence between strains within some regions of the flagellin gene (flaA) of C. jejuni [12][13][14][15]. Differences [16][17][18]. In this study, we present a detailed assessment of flagellin gene typing combined with Lior biotyping and serogrouping by the Penner somatic (0) antigen scheme [19] as an approach to typing a set of contemporary isolates of C. jejuni.…”

Section: Introductionmentioning

confidence: 99%

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Flagellin gene polymorphism analysis ofCampylobacter jejuniinfecting man and other hosts and comparison with biotyping and somatic antigen serotyping

et al. 1994

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SU'MMARYFlagellin gene sequence polymorphisms were used to discriminate amongst 77 strains of Carnpylobacter jejuni from sporadic and outbreak-associated human enteric infections, and from chickens, sheep and calves. The results were assessed in relation to Lior biotyping and serotyping (Penner somatic antigens). Eight DNA PCR-RFLP patterns (genotypes) were identified by analysis of HinfI fragment length polymorphisms in flagellin gene (flaA) polymerase chain reaction (PCR) products. One genotype (F-1) was a feature of 55 % of strains. Strains within the genotypes were heterogeneous with respect to somatic antigens with 12 serogroups represented amongst the C. jejuni isolates offla A type F-1. Serogroups Pen 1, 2 and 23 were the commonest (45 %) amongst the 20 different serogroups represented. Several unique clusters of isolates with diverse biotypes were defined, and one cluster (F-7/Pen 23) contained epidemiologically implicated outbreak strains as well as sheep and calf isolates. We conclude that HinfI fia A typing is reproducible and offers high typability, and its combination with serogrouping provides a novel approach to characterizing isolates of C. jejuni with improved discrimination.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Flagellin gene polymorphism analysis ofCampylobacter jejuniinfecting man and other hosts and comparison with biotyping and somatic antigen serotyping

et al. 1994

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“…Whether the PCR product obtained with a particular strain in this study corresponds to the flaA or flaB gene or both cannot be decided, except for strains where both sequences are available. The Taken on its own, a typing of C. jejuni and C. coli based on RFLP of flagella has been shown to give epidemiologically useful distinctions by several investigators [5][6][7]. The most significant recent addition has been the paper by Owen and colleagues in the pages of this Journal.…”

Section: Resultsmentioning

confidence: 99%

“…Another recently developed approach to typing of C. jejuni and C. coli has been the analysis of polymorphisms among their flagellar genes. The method was based on demonstration of restriction-fragment length polymorphisms (RFLP) of the flagellar genes amplified by the polymerase chain reaction [5][6][7]. In salmonella, where determinants of seroreactivity are well known, a clear correlation was shown to exist between serological reaction of the phase 1 flagella (fliC) and restriction-fragment length polymorphisms of the respective fliC gene, amplified by PCR [8].…”

Section: Introductionmentioning

confidence: 99%

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

et al. 1995

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SUMMARYSeveral typing systems have been described for Campylobacterjejuni and C. coli, to assess the complex epidemiology of these important enteric pathogens. In the present study two typing methods, slide agglutination according to the Lior scheme, and the demonstration of restriction-fragment length polymorphisms (RFLP) of flagellar genes, have been used in parallel on a set of 194 strains. This set comprised 118 sero-reference strains of C. jejuni and C. coli of the Lior scheme, as well as 76 clinical isolates. All isolates were serotyped and subjected to PCR for amplification of flagellar genes, and the PCR product was restricted with Alu I. Flagellar genes could be amplified in 152 strains. Among 85 seroreference strains, 74 different RFLP patterns were observed, and among 67 clinical isolates, there were 36 patterns. There was only limited correlation between flagellar RFLP and the Lior serogroup, and the variability of patterns in serogroups HL2 and HL4 were as marked as the variability between serogroups. Flagellar gene RFLP patterns are shown to be stable, highly discriminatory epidemiologic markers.

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Polymerase Chain Reaction‐Based Subtyping Methods

Chen

Son

2014

DNA Methods in Food Safety

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PCR-based restriction fragment length polymorphism analysis of DNA sequence diversity of flagellin genes of Campylobacter jejuni and allied species

Cited by 23 publications

References 0 publications

Flagellin gene polymorphism analysis ofCampylobacter jejuniinfecting man and other hosts and comparison with biotyping and somatic antigen serotyping

Flagellin gene polymorphism analysis ofCampylobacter jejuniinfecting man and other hosts and comparison with biotyping and somatic antigen serotyping

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

Polymerase Chain Reaction‐Based Subtyping Methods

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