Flagellin gene polymorphism analysis of<i>Campylobacter jejuni</i>infecting man and other hosts and comparison with biotyping and somatic antigen serotyping

Owen, Robert J.; Fitzgerald, Collette; Sutherland, K.; Borman, P.

doi:10.1017/s0950268800051657

Cited by 33 publications

(34 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most significant recent addition has been the paper by Owen and colleagues in the pages of this Journal. They showed for the first time that flagellin gene polymorphisms of human and animal isolates of C. jejuni partially overlap, thereby providing molecular evidence linking human disease with campylobacter carriage in animals, especially poultry [19]. These findings have been corroborated and extended to C. coli by the present study.…”

Section: Resultssupporting

confidence: 83%

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

et al. 1995

View full text Add to dashboard Cite

SUMMARYSeveral typing systems have been described for Campylobacterjejuni and C. coli, to assess the complex epidemiology of these important enteric pathogens. In the present study two typing methods, slide agglutination according to the Lior scheme, and the demonstration of restriction-fragment length polymorphisms (RFLP) of flagellar genes, have been used in parallel on a set of 194 strains. This set comprised 118 sero-reference strains of C. jejuni and C. coli of the Lior scheme, as well as 76 clinical isolates. All isolates were serotyped and subjected to PCR for amplification of flagellar genes, and the PCR product was restricted with Alu I. Flagellar genes could be amplified in 152 strains. Among 85 seroreference strains, 74 different RFLP patterns were observed, and among 67 clinical isolates, there were 36 patterns. There was only limited correlation between flagellar RFLP and the Lior serogroup, and the variability of patterns in serogroups HL2 and HL4 were as marked as the variability between serogroups. Flagellar gene RFLP patterns are shown to be stable, highly discriminatory epidemiologic markers.

show abstract

Section: Resultssupporting

confidence: 83%

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Other molecular techniques have been used for subtyping the three principal C. jejuni HS serogroups (HS 1, 2 and 4), including pulsed-field gel electrophoresis [2,5] and RFLP analysis of the PCRamplified campylobacter fla A gene [19,20]. These studies confirm that within each HS or HL serogroup considerable genetic diversity exists at the loci studied.…”

Section: Discussionmentioning

confidence: 73%

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

et al. 1996

View full text Add to dashboard Cite

SUMMARYCampylobacter jejuni serogroup reference strains and collections of sporadic and outbreakassociated isolates were examined for restriction fragment length polymorphisms (RFLPs), using C. jejuni random chromosomal and 16S rRNA gene probes. A collection of 48 Penner (HS) and 14 Lior (HL) serogroup reference strains, plus 10 clinical isolates, generated 35 RFLP and 26 ribotype patterns. In combination the two loci generated 48 distinct genotypes. Both probes were able to differentiate between certain random isolates of the same HS/HL serogroups but greater discrimination was obtained with RFLP than with ribotyping.

show abstract

“…Several subtyping methods based on flaA have been reported, including PCR-RFLP (19,22) and flaA sequencing (18). They are generally simple, cost-effective, and relatively rapid (2 days).…”

Section: Discussionmentioning

confidence: 99%

“…One sporadic strain (D5497) had a flaA PCR-RFLP profile indistinguishable from that of the outbreak pattern (D1), yet it had a different serotype (O:4) and different PFGE profiles. The occurrence of strains with different serotypes having identical flaA types has been shown previously (22,33). Thus, relying solely on flaA PCR-RFLP analysis can lead to misinterpretation of the data.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Methods for SubtypingCampylobacter jejuniduring an Outbreak Involving a Food Handler

et al. 2001

View full text Add to dashboard Cite

In October 1998, the Centers for Disease Control and Prevention (CDC) assisted in an investigation of an outbreak of campylobacteriosis at a school in Salina, Kansas. Twenty-two isolates were submitted from the Kansas state public health laboratory to CDC, 9 associated with the outbreak and 13 epidemiologically unrelated sporadic isolates. Pulsed-field gel electrophoresis (PFGE) using SmaI and SalI was initially used to validate the epidemiologic data. We then tested the ability of other subtyping techniques to distinguish the outbreak-associated isolates from unrelated sporadic isolates. The methods employed were somatic O serotyping, PCR-restriction fragment length polymorphism (RFLP) analysis of flaA, DNA sequence analysis of 582 bp of flaA that included the short variable region (SVR), and sequencing of the entire flaA gene. PFGE was the most discriminatory technique, yielding 11 SmaI and 10 SalI restriction profiles. All outbreak isolates were indistinguishable by PFGE, somatic O serotyping, and sequencing of the 582-bp region of the flaA gene. fla typing by PCR-RFLP grouped one sporadic isolate with the outbreak strain. Analysis of the DNA sequence of a 582-bp segment of flaA produced strain groupings similar to that generated by PCR-RFLP but further differentiated two flaA PCR-RFLP types (with a 1-bp difference in the 582-bp region). Two sporadic strains were distinct by flaA PCR-RFLP but differed only by a single base substitution in the 582-bp region. The entire flaA gene was sequenced from strains differing by a single base pair in the 582-bp region, and the data revealed that additional discrimination may in some cases be obtained by sequencing outside the SVR. PFGE was superior to all other typing methods tested for strain discrimination; it was crucial for understanding the Kansas outbreak and, when SmaI was used, provided adequate discrimination between unrelated isolates.

show abstract

Flagellin gene polymorphism analysis ofCampylobacter jejuniinfecting man and other hosts and comparison with biotyping and somatic antigen serotyping

Cited by 33 publications

References 21 publications

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains ofCampylobacter jejuniandC. coli

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

Evaluation of Methods for SubtypingCampylobacter jejuniduring an Outbreak Involving a Food Handler

Contact Info

Product

Resources

About